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AB272928

Anti-MADH7/SMAD7 antibody

5

(1 Review)

|

(5 Publications)

Rabbit Polyclonal MADH7/SMAD7 antibody. Suitable for WB, ICC/IF, IHC-Fr and reacts with Human, Rat, Mouse samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human SMAD7.

View Alternative Names

MADH7, MADH8, SMAD7, Mothers against decapentaplegic homolog 7, MAD homolog 7, Mothers against DPP homolog 7, Mothers against decapentaplegic homolog 8, SMAD family member 7, MAD homolog 8, Mothers against DPP homolog 8, SMAD 7, Smad7, hSMAD7

4 Images
Immunohistochemistry (Frozen sections) - Anti-MADH7/SMAD7 antibody (AB272928)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-MADH7/SMAD7 antibody (AB272928)

Frozen sections of mouse cerebellum tissue stained for MADH7/SMAD7 using ab272928 at 1/250 dilution in immunohistochemical analysis (green). Red : NF-H. Blue : DAPI.

Immunocytochemistry/ Immunofluorescence - Anti-MADH7/SMAD7 antibody (AB272928)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MADH7/SMAD7 antibody (AB272928)

4% PFA-fixed rat E18 primary cortical neuron cells stained for MADH7/SMAD7 using ab272928 at 1/500 dilution in ICC/IF (green). Red : Tau. Blue : DAPI.

Western blot - Anti-MADH7/SMAD7 antibody (AB272928)
  • WB

Unknown

Western blot - Anti-MADH7/SMAD7 antibody (AB272928)

10% SDS-PAGE

All lanes:

Western blot - Anti-MADH7/SMAD7 antibody (ab272928) at 1/2000 dilution

Lane 1:

U-87 MG whole cell extract at 30 µg

Lane 2:

SK-N-SH whole cell extract at 30 µg

Lane 3:

IMR32 whole cell extract at 30 µg

Lane 4:

SK-N-AS whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 46 kDa

false

Western blot - Anti-MADH7/SMAD7 antibody (AB272928)
  • WB

Unknown

Western blot - Anti-MADH7/SMAD7 antibody (AB272928)

10% SDS-PAGE

All lanes:

Western blot - Anti-MADH7/SMAD7 antibody (ab272928) at 1/5000 dilution

Lane 1:

Mock transfected HEK-293T cell lysate at 30 µg

Lane 2:

MAD1-transfected HEK-293T cell lysate at 30 µg

Lane 3:

SMAD2 transfected HEK-293T cell lysate at 30 µg

Lane 4:

SMAD3 transfected HEK-293T cell lysate at 30 µg

Lane 5:

SMAD4 transfected HEK-293T cell lysate at 30 µg

Lane 6:

SMAD5 transfected HEK-293T cell lysate at 30 µg

Lane 7:

SMAD7 transfected HEK-293T cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 46 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, WB, IHC-Fr

applications

Immunogen

Synthetic Peptide within Human SMAD7. The exact immunogen used to generate this antibody is proprietary information.

O15105

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/100 - 1/1000", "IHCFr-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MADH7 also known as SMAD7 is an intracellular protein that acts as an inhibitor in the signaling pathway of the TGF-beta (transforming growth factor-beta) family. This protein has a molecular mass of approximately 46 kDa and is widely expressed with a significant presence in tissues involved in immune response and development. SMAD7 directly interacts with receptor-regulated SMADs (R-SMADs) preventing their phosphorylation and consequent nuclear translocation effectively halting further signal transduction.
Biological function summary

SMAD7 functions to regulate the TGF-beta signaling pathway by operating as a negative feedback mediator. It binds to TGF-beta receptors recruiting E3 ubiquitin ligases which promote receptor degradation. SMAD7 is not part of complex large-scale multi-protein assemblies but is essential in modulating the pathway's activity. Its balance controls important processes like cell proliferation differentiation and apoptosis across varied biological contexts.

Pathways

SMAD7 strongly influences the TGF-beta and BMP (bone morphogenetic protein) signaling pathways. SMAD7 blocks TGF-beta signaling by interfering with the activity of R-SMADs such as SMAD2 and SMAD3 and it modulates the BMP pathway by interacting with SMAD1 and SMAD5. Its regulatory roles are tightly integrated within these pathways highlighting its importance in cellular homeostasis and response to extracellular signals.

SMAD7 has links to inflammatory conditions and cancer. Its dysregulation can lead to heightened TGF-beta signaling which may contribute to conditions like fibrosis and can enhance tumor progression by affecting cancer cell dynamics and the tumor microenvironment. In these scenarios abnormal SMAD7 function can associate with proteins such as SMAD3 in fibrosis while in cancer its association with proteins like SMAD4 disrupts normal growth control and cellular response.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Antagonist of signaling by TGF-beta (transforming growth factor) type 1 receptor superfamily members; has been shown to inhibit TGF-beta (Transforming growth factor) and activin signaling by associating with their receptors thus preventing SMAD2 access (PubMed : 21791611). Functions as an adapter to recruit SMURF2 to the TGF-beta receptor complex. Also acts by recruiting the PPP1R15A-PP1 complex to TGFBR1, which promotes its dephosphorylation. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.
See full target information SMAD7
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 14:10340 PubMed38710764

2024

Trefoil factor 3 can stimulate Th17 cell response in the development of type 2 diabetes mellitus.

Applications

Unspecified application

Species

Unspecified reactive species

Ziyang Lin,Jinyuan Zhang,Tingting Duan,Junzheng Yang,Yiqi Yang

Cancer research 83:3095-3114 PubMed37350664

2023

ESRRB Inhibits the TGFβ Signaling Pathway to Drive Cell Proliferation in Cervical Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Qin-Shu Li,Peng-Sheng Zheng

Molecular therapy : the journal of the American Society of Gene Therapy 31:1313-1331 PubMed36739479

2023

Astrocyte-derived exosomal lncRNA 4933431K23Rik modulates microglial phenotype and improves post-traumatic recovery via SMAD7 regulation.

Applications

Unspecified application

Species

Unspecified reactive species

Xuejun He,Yimin Huang,Yuan Liu,Xincheng Zhang,Quanji Wang,Yanchao Liu,Xiaopeng Ma,Xiaobing Long,Yang Ruan,Hongxia Lei,Chao Gan,Xiaochuan Wang,Xin Zou,Bo Xiong,Kai Shu,Ting Lei,Huaqiu Zhang

Experimental and therapeutic medicine 24:486 PubMed35761808

2022

Tanshinone IIA regulates the TGF-β1/Smad signaling pathway to ameliorate non-alcoholic steatohepatitis-related fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Lianjie Xu,Yurong Zhang,Nengbo Ji,Yan Du,Tao Jia,Shanshan Wei,Wei Wang,Shan Zhang,Wenhui Chen

Molecular medicine reports 25: PubMed35029288

2022

Total glucosides of paeony ameliorates oxidative stress, apoptosis and inflammatory response by regulating the Smad7‑TGF‑β pathway in allergic rhinitis.

Applications

Unspecified application

Species

Unspecified reactive species

Yangzi Jin,Aichun Zhang
View all publications
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