AB90086

Anti-MADH7/SMAD7 antibody

Name: Anti-MADH7/SMAD7 antibody (ab90086) | Abcam
Brand: Abcam Limited
SKU: AB90086
Price: 535 USD
Availability: InStock

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(30 Publications)

Rabbit Polyclonal MADH7/SMAD7 antibody. Suitable for WB and reacts with Human samples. Cited in 30 publications. Immunogen corresponding to Synthetic Peptide within Human SMAD7.

View Alternative Names

MADH7, MADH8, SMAD7, SMAD family member 7, SMAD 7, hSMAD7, Mothers against decapentaplegic homolog 7, Mothers against decapentaplegic homolog 8, MAD homolog 7, Mothers against DPP homolog 7, MAD homolog 8, Mothers against DPP homolog 8

2 Images

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Western blot - Anti-MADH7/SMAD7 antibody (AB90086)

All lanes:

Western blot - Anti-MADH7/SMAD7 antibody (ab90086) at 1/500 dilution

All lanes:

HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate

Predicted band size: 46 kDa

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CiteAb

Western blot - Anti-MADH7/SMAD7 antibody (AB90086)

MADH7/SMAD7 western blot using anti-MADH7/SMAD7 antibody ab90086. Publication image and figure legend from Zhang, F., Ren, C. C., et al., 2018, Cancer Cell Int, PubMed 30559605.

ab90086 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab90086 please see the product overview.

HOXC6 gene silencing inhibits EMT and proliferative ability of CC cells by suppressing the activation of the TGF-β/smad signaling pathway. a Protein expression of HOXC6, TGF-β1, TGF-β RII, smad4, smad7, E-cadherin, N-cadherin, and Vimentin determined by western blot analysis; b cell proliferation in each group after transduction; c cell cycle in each group after transduction; d cell apoptosis in each group after transduction; *p < 0.05, compared with the HOXC6-siRNA + HA-NC group; #p < 0.05, compared with the HA-HOXC6 + si-NC group; HOXC6, homeobox C6; EMT, epithelial-mesenchymal transition; CC cervical carcinoma, TGF-β transforming growth factor-β, smad solvated metal atom dispersion, NC negative control

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Synthetic Peptide within Human SMAD7. The exact immunogen used to generate this antibody is proprietary information.

O15105

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/1000", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Lyophilized

Reconstitution

reconstitute with water at 200µL

Purification technique

Affinity purification Immunogen

Storage buffer

Preservative: 0.02% Sodium azide Constituents: 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MADH7 also known as SMAD7 is an intracellular protein that acts as an inhibitor in the signaling pathway of the TGF-beta (transforming growth factor-beta) family. This protein has a molecular mass of approximately 46 kDa and is widely expressed with a significant presence in tissues involved in immune response and development. SMAD7 directly interacts with receptor-regulated SMADs (R-SMADs) preventing their phosphorylation and consequent nuclear translocation effectively halting further signal transduction.

Biological function summary

SMAD7 functions to regulate the TGF-beta signaling pathway by operating as a negative feedback mediator. It binds to TGF-beta receptors recruiting E3 ubiquitin ligases which promote receptor degradation. SMAD7 is not part of complex large-scale multi-protein assemblies but is essential in modulating the pathway's activity. Its balance controls important processes like cell proliferation differentiation and apoptosis across varied biological contexts.

Pathways

SMAD7 strongly influences the TGF-beta and BMP (bone morphogenetic protein) signaling pathways. SMAD7 blocks TGF-beta signaling by interfering with the activity of R-SMADs such as SMAD2 and SMAD3 and it modulates the BMP pathway by interacting with SMAD1 and SMAD5. Its regulatory roles are tightly integrated within these pathways highlighting its importance in cellular homeostasis and response to extracellular signals.

SMAD7 has links to inflammatory conditions and cancer. Its dysregulation can lead to heightened TGF-beta signaling which may contribute to conditions like fibrosis and can enhance tumor progression by affecting cancer cell dynamics and the tumor microenvironment. In these scenarios abnormal SMAD7 function can associate with proteins such as SMAD3 in fibrosis while in cancer its association with proteins like SMAD4 disrupts normal growth control and cellular response.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Antagonist of signaling by TGF-beta (transforming growth factor) type 1 receptor superfamily members; has been shown to inhibit TGF-beta (Transforming growth factor) and activin signaling by associating with their receptors thus preventing SMAD2 access (PubMed : 21791611). Functions as an adapter to recruit SMURF2 to the TGF-beta receptor complex. Also acts by recruiting the PPP1R15A-PP1 complex to TGFBR1, which promotes its dephosphorylation. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.

See full target information SMAD7

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Publications (30)

Recent publications for all applications. Explore the full list and refine your search

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 38:e23369 PubMed38100642

2023

Vascular smooth muscle cell-specific miRNA-214 deficiency alleviates simulated microgravity-induced vascular remodeling.

Applications

Unspecified application

Species

Unspecified reactive species

Youyou Li,Yunzhang Zhao,Guohui Zhong,Qing Xu,Yingjun Tan,Wenjuan Xing,Dengchao Cao,Yinbo Wang,Caizhi Liu,Jianwei Li,Ruikai Du,Weijia Sun,Xinxin Yuan,Yeheng Li,Zizhong Liu,Xiaoyan Jin,Dingsheng Zhao,Jinping Song,Yanqing Wang,Guanghan Kan,Xuan Han,Shujuan Liu,Min Yuan,Feng Gao,Jingdan Shu,Yingxian Li,Shukuan Ling

Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas 55:e12206 PubMed36629522

2023

Immune suppressive signaling regulated by latent transforming growth factor beta binding protein 1 promotes metastasis in cervical cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Haiyan Gu,Wei Wang,Changdong Sun,Li Ding,Li Li,Peng Shu,Jun Xu

International journal of molecular sciences 23: PubMed36077357

2022

Caffeine Inhibits NLRP3 Inflammasome Activation by Downregulating TLR4/MAPK/NF-κB Signaling Pathway in an Experimental NASH Model.

Applications

Unspecified application

Species

Unspecified reactive species

Eduardo E Vargas-Pozada,Erika Ramos-Tovar,Juan D Rodriguez-Callejas,Irina Cardoso-Lezama,Silvia Galindo-Gómez,Daniel Talamás-Lara,Verónica Rocío Vásquez-Garzón,Jaime Arellanes-Robledo,Víctor Tsutsumi,Saúl Villa-Treviño,Pablo Muriel

Molecular medicine reports 25: PubMed35137923

2022

Molecular mechanism of the TGF‑β/Smad7 signaling pathway in ulcerative colitis.

Applications

Unspecified application

Species

Unspecified reactive species

Bingqing Bai,Huihui Li,Liang Han,Yongyu Mei,Cui Hu,Qiao Mei,Jianming Xu,Xiaochang Liu

PeerJ 9:e12686 PubMed35036160

2021

miR-15a-5p regulates myocardial fibrosis in atrial fibrillation by targeting Smad7.

Applications

Unspecified application

Species

Unspecified reactive species

Dan He,Zhong-Bao Ruan,Gui-Xian Song,Ge-Cai Chen,Fei Wang,Mei-Xiang Wang,Mao-Kun Yuan,Li Zhu

Cancer management and research 13:7087-7099 PubMed34531686

2021

TGF-β-MTA1-SMAD7-SMAD3-SOX4-EZH2 Signaling Axis Promotes Viability, Migration, Invasion and EMT of Hepatocellular Carcinoma Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Kangjun Zhang,Taishi Fang,Yajie Shao,Yanhui Wu

Molecular and cellular biochemistry 476:3051-3064 PubMed33797702

2021

Downregulation of miR-17 suppresses TGF-β1-mediated renal fibrosis through targeting Smad7.

Applications

Unspecified application

Species

Unspecified reactive species

Haixia Fu,Debo Chu,Xiuli Geng

Molecular therapy. Nucleic acids 22:114-123 PubMed32916597

2020

ROS/NF-κB Signaling Pathway-Mediated Transcriptional Activation of TRIM37 Promotes HBV-Associated Hepatic Fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Haina Xie,Dongyu Xie,Jingxian Zhang,Wenye Jin,Yue Li,Jun Yao,Zhiqiang Pan,Donghao Xie

Medical science monitor : international medical jo 26:e920736 PubMed32308208

2020

The Effects of the Transforming Growth Factor-β1 (TGF-β1) Signaling Pathway on Cell Proliferation and Cell Migration are Mediated by Ubiquitin Specific Protease 4 (USP4) in Hypertrophic Scar Tissue and Primary Fibroblast Cultures.

Applications

Unspecified application

Species

Unspecified reactive species

Yong Huang,Yuting Wang,Xueming Wang,Lixin Lin,Peng Wang,Junjun Sun,Lei Jiang

Stem cell research & therapy 11:117 PubMed32171324

2020

MicroRNA-495 confers inhibitory effects on cancer stem cells in oral squamous cell carcinoma through the HOXC6-mediated TGF-β signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaolong You,Zhengyu Zhou,Wen Chen,Xiaoyong Wei,Heqiang Zhou,Wenzheng Luo

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Anti-MADH7/SMAD7 antibody

Western blot - Anti-MADH7/SMAD7 antibody (AB90086)

Western blot - Anti-MADH7/SMAD7 antibody (AB90086)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Reconstitution

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (30)

Product promise