AB151304

Anti-MAEA antibody

Name: Anti-MAEA antibody (ab151304) | Abcam
Brand: Abcam Limited
SKU: AB151304
Price: 535 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal MAEA antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human MAEA aa 150 to C-terminus.

View Alternative Names

EMP, HLC10, PIG5, MAEA, E3 ubiquitin-protein transferase MAEA, Cell proliferation-inducing gene 5 protein, Erythroblast macrophage protein, Human lung cancer oncogene 10 protein, Macrophage erythroblast attacher, P44EMLP, HLC-10

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-MAEA antibody (AB151304)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-MAEA antibody (AB151304)

Immunofluorescent analysis using confocal microscopy of methanol-fixed HeLa cells labeling MAEA with ab151304 antibody at 1/500 dilution (Green). Alpha-tubulin filaments were labeled in Red.

Unknown

Western blot - Anti-MAEA antibody (AB151304)

10% SDS PAGE

All lanes:

Western blot - Anti-MAEA antibody (ab151304) at 1/5000 dilution

All lanes:

HepG2 whole cell lysate at 30 µg

Predicted band size: 45 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human MAEA aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q7L5Y9

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p>Methanol fixed.</p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Xenopus tropicalis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Zebrafish": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MAEA also known as Macrophage Erythroblast Attacher is a protein with a mass of approximately 42 kDa. This protein functions mechanically by anchoring mature erythroblasts to macrophages in the erythroblastic islands of the bone marrow. You can find MAEA expression in hematopoietic organs such as the spleen bone marrow and lymph nodes. Its expression is not limited to these sites since several tissues exhibit lower levels of expression indicating a broader biological role.

Biological function summary

MAEA plays a significant role in hematopoiesis by facilitating communication between macrophages and erythroblasts. It forms part of a protein complex that ensures the stability and viability of developing erythrocytes. This protein interaction is critical for the normal maturation of red blood cells helping to maintain the balance and production of these cells under varying physiological conditions.

Pathways

MAEA participates in the erythrophagocytosis and erythroblast enucleation processes. MAEA contributes to the interaction with proteins such as ICAM-4 and VLA-4 which are important in these pathways. Through these interactions MAEA is instrumental in effectively mediating red blood cell clearance and differentiation ensuring the proper function of erythroblastic islands within the bone marrow niche.

MAEA links to pathologies such as anemia and myeloproliferative disorders. Altered MAEA expression or function can disturb normal erythropoiesis leading to ineffective red blood cell production and anemia. Additionally its interaction with proteins like ICAM-4 can affect disease progression and severity. Researchers continue to study these connections to understand MAEA’s role in hematological disorders better.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Core component of the CTLH E3 ubiquitin-protein ligase complex that selectively accepts ubiquitin from UBE2H and mediates ubiquitination and subsequent proteasomal degradation of the transcription factor HBP1. MAEA and RMND5A are both required for catalytic activity of the CTLH E3 ubiquitin-protein ligase complex (PubMed : 29911972). MAEA is required for normal cell proliferation (PubMed : 29911972). The CTLH E3 ubiquitin-protein ligase complex is not required for the degradation of enzymes involved in gluconeogenesis, such as FBP1 (PubMed : 29911972). Plays a role in erythroblast enucleation during erythrocyte maturation and in the development of mature macrophages (By similarity). Mediates the attachment of erythroid cell to mature macrophages; this MAEA-mediated contact inhibits erythroid cell apoptosis (PubMed : 9763581). Participates in erythroblastic island formation, which is the functional unit of definitive erythropoiesis. Associates with F-actin to regulate actin distribution in erythroblasts and macrophages (By similarity). May contribute to nuclear architecture and cells division events (Probable).

See full target information MAEA

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

BMC cancer 17:460 PubMed28668087

2017

Inhibition of HDAC6 activity through interaction with RanBPM and its associated CTLH complex.

Applications

Unspecified application

Species

Unspecified reactive species

Louisa M Salemi,Matthew E R Maitland,Eyal R Yefet,Caroline Schild-Poulter

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MAEA antibody

Immunocytochemistry/ Immunofluorescence - Anti-MAEA antibody (AB151304)

Western blot - Anti-MAEA antibody (AB151304)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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