AB232987

Anti-MAEA antibody

Name: Anti-MAEA antibody (ab232987) | Abcam
Brand: Abcam Limited
SKU: AB232987

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(1 Publication)

Rabbit Polyclonal MAEA antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human MAEA aa 100 to C-terminus.

View Alternative Names

EMP, HLC10, PIG5, MAEA, E3 ubiquitin-protein transferase MAEA, Cell proliferation-inducing gene 5 protein, Erythroblast macrophage protein, Human lung cancer oncogene 10 protein, Macrophage erythroblast attacher, P44EMLP, HLC-10

10 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAEA antibody (AB232987)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAEA antibody (AB232987)

Paraffin-embedded human bile duct cancer tissue stained for MAEA using ab232987 at 20 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAEA antibody (AB232987)

Paraffin-embedded human colorectal cancer tissue stained for MAEA using ab232987 at 20 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAEA antibody (AB232987)

Paraffin-embedded human breast cancer tissue stained for MAEA using ab232987 at 20 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAEA antibody (AB232987)

Paraffin-embedded human cerebrum tissue stained for MAEA using ab232987 at 20 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAEA antibody (AB232987)

Paraffin-embedded human skin cancer tissue stained for MAEA using ab232987 at 20 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAEA antibody (AB232987)

Formalin-fixed, paraffin-embedded human stomach tissue stained for MAEA using ab232987 at 20 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAEA antibody (AB232987)

Paraffin-embedded human kidney tissue stained for MAEA using ab232987 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Supplier Data

Western blot - Anti-MAEA antibody (AB232987)

All lanes:

Western blot - Anti-MAEA antibody (ab232987) at 2 µg/mL

All lanes:

Recombinant human MAEA protein

Predicted band size: 45 kDa

false

Supplier Data

Western blot - Anti-MAEA antibody (AB232987)

All lanes:

Western blot - Anti-MAEA antibody (ab232987) at 2 µg/mL

All lanes:

Mouse pancreas lysate

Predicted band size: 45 kDa

false

Supplier Data

Western blot - Anti-MAEA antibody (AB232987)

All lanes:

Western blot - Anti-MAEA antibody (ab232987) at 2 µg/mL

All lanes:

Mouse cerebrum lysate

Predicted band size: 45 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human MAEA aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q7L5Y9

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cynomolgus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Zebrafish": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab232987 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.

Storage buffer

pH: 7.4 Preservative: 0.011% Proclin 300 Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MAEA also known as Macrophage Erythroblast Attacher is a protein with a mass of approximately 42 kDa. This protein functions mechanically by anchoring mature erythroblasts to macrophages in the erythroblastic islands of the bone marrow. You can find MAEA expression in hematopoietic organs such as the spleen bone marrow and lymph nodes. Its expression is not limited to these sites since several tissues exhibit lower levels of expression indicating a broader biological role.

Biological function summary

MAEA plays a significant role in hematopoiesis by facilitating communication between macrophages and erythroblasts. It forms part of a protein complex that ensures the stability and viability of developing erythrocytes. This protein interaction is critical for the normal maturation of red blood cells helping to maintain the balance and production of these cells under varying physiological conditions.

Pathways

MAEA participates in the erythrophagocytosis and erythroblast enucleation processes. MAEA contributes to the interaction with proteins such as ICAM-4 and VLA-4 which are important in these pathways. Through these interactions MAEA is instrumental in effectively mediating red blood cell clearance and differentiation ensuring the proper function of erythroblastic islands within the bone marrow niche.

MAEA links to pathologies such as anemia and myeloproliferative disorders. Altered MAEA expression or function can disturb normal erythropoiesis leading to ineffective red blood cell production and anemia. Additionally its interaction with proteins like ICAM-4 can affect disease progression and severity. Researchers continue to study these connections to understand MAEA’s role in hematological disorders better.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Core component of the CTLH E3 ubiquitin-protein ligase complex that selectively accepts ubiquitin from UBE2H and mediates ubiquitination and subsequent proteasomal degradation of the transcription factor HBP1. MAEA and RMND5A are both required for catalytic activity of the CTLH E3 ubiquitin-protein ligase complex (PubMed : 29911972). MAEA is required for normal cell proliferation (PubMed : 29911972). The CTLH E3 ubiquitin-protein ligase complex is not required for the degradation of enzymes involved in gluconeogenesis, such as FBP1 (PubMed : 29911972). Plays a role in erythroblast enucleation during erythrocyte maturation and in the development of mature macrophages (By similarity). Mediates the attachment of erythroid cell to mature macrophages; this MAEA-mediated contact inhibits erythroid cell apoptosis (PubMed : 9763581). Participates in erythroblastic island formation, which is the functional unit of definitive erythropoiesis. Associates with F-actin to regulate actin distribution in erythroblasts and macrophages (By similarity). May contribute to nuclear architecture and cells division events (Probable).

See full target information MAEA

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of clinical oncology 29:771-779 PubMed38600426

2024

Progressive changes in the protein expression profile of alveolar septa in early-stage lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Toru Kimura,Takashi Akazawa,Yu Mizote,Harumi Nakamura,Miki Sakaue,Tomohiro Maniwa,Yasushi Shintani,Keiichiro Honma,Hideaki Tahara,Jiro Okami

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MAEA antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAEA antibody (AB232987)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAEA antibody (AB232987)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAEA antibody (AB232987)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAEA antibody (AB232987)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAEA antibody (AB232987)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAEA antibody (AB232987)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAEA antibody (AB232987)

Western blot - Anti-MAEA antibody (AB232987)

Western blot - Anti-MAEA antibody (AB232987)

Western blot - Anti-MAEA antibody (AB232987)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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