AB106713

Anti-Mael antibody

Name: Anti-Mael antibody (ab106713) | Abcam
Brand: Abcam Limited
SKU: AB106713
Price: 545 USD
Availability: InStock
Rating: 1 (1 reviews)

(1 Review)

(4 Publications)

Rabbit Polyclonal MAEL antibody. Suitable for ICC, WB, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human MAEL.

View Alternative Names

Protein maelstrom homolog, MAEL

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-Mael antibody (AB106713)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Mael antibody (AB106713)

Immunofluorescence of Mael in HeLa cells with ab106713 at 20 ug/mL.

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Immunocytochemistry - Anti-Mael antibody (AB106713)

ab106713, at 5 μg/ml, staining in Hela cells with Maelstrom by Immunocytochemistry.

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Western blot - Anti-Mael antibody (AB106713)

All lanes:

Western blot - Anti-Mael antibody (ab106713) at 1 µg/mL

All lanes:

HeLa cell lysate at 15 µg

Predicted band size: 49 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC, WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human MAEL. The exact immunogen used to generate this antibody is proprietary information.

Q96JY0

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "5 µg/mL", "ICC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "20 µg/mL", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

Up to 12 months

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Mael also known as Maelstrom is a protein associated with the Piwi-interacting RNA (piRNA) pathway. This protein has a mass of approximately 52 kDa. Mael is mainly expressed in germ cells where it contributes to spermatogenesis. It acts as an important component of the piRNA pathway which is important to defend the genome integrity in germline cells against transposable elements. Its function is essential for germ cell development and integrity.

Biological function summary

Maelstrom interacts with other proteins to form a complex necessary for the piRNA biogenesis. This complex aids in the silencing of transposable elements to protect germ cell DNA from damage. Understanding Mael's role within the piRNA pathway highlights its significance in regulating genomic stability. It acts in coordination with other piRNA pathway proteins like Piwi and Tudor domain proteins to ensure effective transposon silencing. These interactions emphasize Mael’s importance for maintaining the stability of genetic information passed to the next generation.

Pathways

Mael plays an integral part in the piRNA-mediated silencing pathway. It participates in piRNA biogenesis an important pathway involved in post-transcriptional regulation of gene expression in the germline. Mael has a relation to other pathway-associated proteins such as Ago (Argonaute proteins) and Piwi proteins which are fundamental for transcriptional silencing and transposon control. This association with piRNA machinery highlights Mael's role in safeguarding gamete DNA from transposon-induced mutations.

Research indicates that Mael contributes to infertility linked with anomalies in spermatogenesis. Aberrant function or expression of Mael can lead to defective piRNA processing which compromises genome integrity and results in infertility. Its link with proteins like Piwi proteins in regulating transposons also suggests a potential connection to testicular cancer as disruptions in piRNA pathways can contribute to oncogenic processes within germ cells. Investigating Mael and its interactions provides insights into therapeutic approaches for treating such reproductive disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Plays a central role during spermatogenesis by repressing transposable elements and preventing their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons. Its association with piP-bodies suggests a participation in the secondary piRNAs metabolic process. Required for the localization of germ-cell factors to the meiotic nuage (By similarity).

See full target information MAEL

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

BMC pharmacology & toxicology 23:92 PubMed36476246

2022

Morphine suppresses the immune function of lung cancer by up-regulating MAEL expression.

Applications

Unspecified application

Species

Unspecified reactive species

Qichao Wang,Zhenfu Liu,Shuhong Tang,Zhen Wu

PloS one 14:e0217941 PubMed31181099

2019

Expression of genome defence protein members in proliferating and quiescent rat male germ cells and the Nuage dynamics.

Applications

Unspecified application

Species

Unspecified reactive species

Letícia Rocha-da-Silva,Lucia Armelin-Correa,Isabelle Hernandez Cantão,Verena Julia Flaiz Flister,Marina Nunes,Taiza Stumpp

Stem cells and development 27:723-735 PubMed29631484

2018

Quantitative Proteomic Profiling of the Human Ovary from Early to Mid-Gestation Reveals Protein Expression Dynamics of Oogenesis and Folliculogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Alisha M Bothun,Yuanwei Gao,Yasushi Takai,Osamu Ishihara,Hiroyuki Seki,Barry Karger,Jonathan L Tilly,Dori C Woods

Molecular medicine reports 13:2829-35 PubMed26847393

2016

High expression of piwi-like RNA-mediated gene silencing 1 is associated with poor prognosis via regulating transforming growth factor-β receptors and cyclin-dependent kinases in breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jiwei Cao,Gang Xu,Jing Lan,Qingqing Huang,Zuxiong Tang,Liping Tian

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Mael antibody

Immunocytochemistry/ Immunofluorescence - Anti-Mael antibody (AB106713)

Immunocytochemistry - Anti-Mael antibody (AB106713)

Western blot - Anti-Mael antibody (AB106713)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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