Mouse Polyclonal MAF1 antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human MAF1.
Images
Key facts
- Isotype
IgG
- Host species
Mouse
- Storage buffer
pH: 7.4
Constituents: 99% PBS- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human MAF1. Database link Q9H063
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
Associated Products
Select an associated product type
1 products for Alternative Product
Target data
Function
Plays a role in the repression of RNA polymerase III-mediated transcription in response to changing nutritional, environmental and cellular stress conditions to balance the production of highly abundant tRNAs, 5S rRNA, and other small non-coding RNAs with cell growth and maintenance (PubMed:18377933, PubMed:20233713, PubMed:20516213, PubMed:20543138). Plays also a key role in cell fate determination by promoting mesorderm induction and adipocyte differentiation (By similarity). Mechanistically, associates with the RNA polymerase III clamp and thereby impairs its recruitment to the complex made of the promoter DNA, TBP and the initiation factor TFIIIB (PubMed:20887893, PubMed:17505538). When nutrients are available and mTOR kinase is active, MAF1 is hyperphosphorylated and RNA polymerase III is engaged in transcription. Stress-induced MAF1 dephosphorylation results in nuclear localization, increased targeting of gene-bound RNA polymerase III and a decrease in the transcriptional readout (PubMed:26941251). Additionally, may also regulate RNA polymerase I and RNA polymerase II-dependent transcription through its ability to regulate expression of the central initiation factor TBP (PubMed:17499043).
Alternative names
Repressor of RNA polymerase III transcription MAF1 homolog, MAF1
Recommended products
Mouse Polyclonal MAF1 antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human MAF1.
Alternative names
Repressor of RNA polymerase III transcription MAF1 homolog, MAF1
Key facts
- Isotype
IgG
- Host species
Mouse
- Storage buffer
pH: 7.4
Constituents: 99% PBS- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human MAF1. Database link Q9H063
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- Activity summary
MAF1 also known as MAF1 homolog is a negative regulator of RNA polymerase III an important enzyme in the synthesis of small RNAs such as tRNA and 5S rRNA. The molecular mass of MAF1 is approximately 29 kDa. This protein mainly expresses in the nucleus of eukaryotic cells. MAF1's activity is controlled by phosphorylation which governs its ability to repress the transcription of RNA polymerase III.
- Biological function summary
MAF1 is significant in cellular growth and metabolism regulation. It is not part of a larger protein complex but interacts with several proteins to exert its regulatory functions. MAF1 acts as a brake on RNA polymerase III activity responding to changes in cellular environmental conditions such as nutrient availability and stress. By doing so MAF1 helps coordinate cellular responses to shifts in energy levels and stress modulating the balance between cell growth and maintenance.
- Pathways
MAF1 plays a role in the mTOR signaling pathway which is important for cell growth proliferation and survival. Within this pathway MAF1 functions as a downstream effector that inhibits RNA transcription when mTORC1 activity decreases. It relates closely to other proteins such as mTOR and AKT which are also involved in cellular responses to nutrient and energy fluctuations. By influencing RNA polymerase III transcription MAF1 contributes to the regulation of protein synthesis according to metabolic needs.
- Associated diseases and disorders
Alterations in MAF1 activity associate with cancer and metabolic syndromes. In cancer reduced MAF1 activity can lead to unchecked RNA polymerase III activity promoting fast cell proliferation and tumor growth. In metabolic disorders MAF1's involvement in nutrient sensing pathways links to insulin resistance when dysregulated. Through these conditions MAF1 connects to proteins like mTOR and AKT and its malfunction can exacerbate disease progression due to disrupted cell growth control.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
Western blot - Anti-MAF1 antibody (ab167235)
All lanes: Western blot - Anti-MAF1 antibody (ab167235) at 1 µg/mL
Lane 1: MAF1 transfected 293T cell lysate at 15 µL
Lane 2: Non-transfected 293T cell lysate at 15 µL
SecondaryAll lanes: Goat anti-Mouse IgG HRP at 1/2500 dilution
Predicted band size: 26 kDa, 27 kDa, 28 kDa, 29 kDa, 30 kDa, 33 kDa, 35 kDa, 38 kDa, 39 kDa, 40 kDa, 41 kDa, 42 kDa, 43 kDa, 45 kDa, 47 kDa, 48 kDa, 51 kDa, 52 kDa, 53 kDa, 54 kDa, 55 kDa, 57 kDa, 58 kDa, 62 kDa, 77 kDa, 84 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com