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AB230499

Anti-MAF1 antibody

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(3 Publications)

Rabbit Polyclonal MAF1 antibody. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human MAF1 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

Repressor of RNA polymerase III transcription MAF1 homolog, MAF1

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAF1 antibody (AB230499)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAF1 antibody (AB230499)

Formalin-fixed, paraffin-embedded human colon tissue stained for MAF1 using ab230499 at 1/100 dilution in immunohistochemical analysis.

Western blot - Anti-MAF1 antibody (AB230499)
  • WB

Supplier Data

Western blot - Anti-MAF1 antibody (AB230499)

All lanes:

Western blot - Anti-MAF1 antibody (ab230499) at 1/500 dilution

Lane 1:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate

Lane 2:

Raw264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate

Lane 3:

H9c2 (Rat heart myoblast cell line) whole cell lysate

Predicted band size: 29 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human MAF1 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q9H063

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.3 Preservative: 0.01% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MAF1 also known as MAF1 homolog is a negative regulator of RNA polymerase III an important enzyme in the synthesis of small RNAs such as tRNA and 5S rRNA. The molecular mass of MAF1 is approximately 29 kDa. This protein mainly expresses in the nucleus of eukaryotic cells. MAF1's activity is controlled by phosphorylation which governs its ability to repress the transcription of RNA polymerase III.
Biological function summary

MAF1 is significant in cellular growth and metabolism regulation. It is not part of a larger protein complex but interacts with several proteins to exert its regulatory functions. MAF1 acts as a brake on RNA polymerase III activity responding to changes in cellular environmental conditions such as nutrient availability and stress. By doing so MAF1 helps coordinate cellular responses to shifts in energy levels and stress modulating the balance between cell growth and maintenance.

Pathways

MAF1 plays a role in the mTOR signaling pathway which is important for cell growth proliferation and survival. Within this pathway MAF1 functions as a downstream effector that inhibits RNA transcription when mTORC1 activity decreases. It relates closely to other proteins such as mTOR and AKT which are also involved in cellular responses to nutrient and energy fluctuations. By influencing RNA polymerase III transcription MAF1 contributes to the regulation of protein synthesis according to metabolic needs.

Alterations in MAF1 activity associate with cancer and metabolic syndromes. In cancer reduced MAF1 activity can lead to unchecked RNA polymerase III activity promoting fast cell proliferation and tumor growth. In metabolic disorders MAF1's involvement in nutrient sensing pathways links to insulin resistance when dysregulated. Through these conditions MAF1 connects to proteins like mTOR and AKT and its malfunction can exacerbate disease progression due to disrupted cell growth control.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a role in the repression of RNA polymerase III-mediated transcription in response to changing nutritional, environmental and cellular stress conditions to balance the production of highly abundant tRNAs, 5S rRNA, and other small non-coding RNAs with cell growth and maintenance (PubMed : 18377933, PubMed : 20233713, PubMed : 20516213, PubMed : 20543138). Also plays a key role in cell fate determination by promoting mesorderm induction and adipocyte differentiation (By similarity). Mechanistically, associates with the RNA polymerase III clamp and thereby impairs its recruitment to the complex made of the promoter DNA, TBP and the initiation factor TFIIIB (PubMed : 17505538, PubMed : 20887893). When nutrients are available and mTOR kinase is active, MAF1 is hyperphosphorylated and RNA polymerase III is engaged in transcription. Stress-induced MAF1 dephosphorylation results in nuclear localization, increased targeting of gene-bound RNA polymerase III and a decrease in the transcriptional readout (PubMed : 26941251). Additionally, may also regulate RNA polymerase I and RNA polymerase II-dependent transcription through its ability to regulate expression of the central initiation factor TBP (PubMed : 17499043).
See full target information MAF1
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Carcinogenesis 45:475-486 PubMed38366633

2024

LMO3 is a suppressor of the basal-like/squamous subtype and reduces disease aggressiveness of pancreatic cancer through glycerol 3-phosphate metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Yuuki Ohara,Amanda J Craig,Huaitian Liu,Shouhui Yang,Paloma Moreno,Tiffany H Dorsey,Helen Cawley,Azadeh Azizian,Jochen Gaedcke,Michael Ghadimi,Nader Hanna,Stefan Ambs,S Perwez Hussain

Brain : a journal of neurology 147:2128-2143 PubMed38226680

2024

Maf1 loss regulates spinogenesis and attenuates cognitive impairment in Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Yingying Han,Kui Chen,Hongxiang Yu,Can Cui,Hongxia Li,Yongbo Hu,Bei Zhang,Gang Li

Frontiers in immunology 11:594071 PubMed33424842

2020

Maf1 Ameliorates Sepsis-Associated Encephalopathy by Suppressing the NF-B/NLRP3 Inflammasome Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Shenglong Chen,Chaogang Tang,Hongguang Ding,Zhonghua Wang,Xinqiang Liu,Yunfei Chai,Wenqiang Jiang,Yongli Han,Hongke Zeng
View all publications
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Product promise

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