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Rabbit Polyclonal MAFA antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 18 publications.


Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MafA antibody (AB26405), expandable thumbnail
  • Western blot - Anti-MafA antibody (AB26405), expandable thumbnail
  • Western blot - Anti-MafA antibody (AB26405), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA

Form

Liquid

Clonality

Polyclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Consider this alternative

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IF
Human
Tested
Tested
Expected
Mouse
Predicted
Predicted
Predicted

Tested
Tested

Species

Human

Dilution info

1 µg/mL

Notes

Human MAFA has a predicted molecular weight of 37 kDa (Swiss-Prot data), however publications have shown the human protein to be detected at 47 kDa according to reference PMID 12011435, http://www.pnas.org/cgi/content/full/99/10/6737. Our data shows ab26405 to detect a band at 36 kDa in human brain, kidney and liver which is similar to that seen in other publications (PMID:14680841, PMID:18159220 (expression profile) ). The mouse protein has also been observed at 47 kDa according to reference PMID 12368292.

Predicted
Predicted

Species

Mouse

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

0.8-4 µg/mL

Notes

-

Predicted
Predicted

Species

Mouse

Dilution info

-

Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Mouse

Dilution info

-

Notes

-

Associated Products

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Target data

Function

Transcription factor that activates insulin gene expression (PubMed:12011435, PubMed:15993959). Acts synergistically with NEUROD1/BETA2 and PDX1 (PubMed:15993959). Binds the insulin enhancer C1/RIPE3b element (PubMed:12011435). Binds to consensus TRE-type MARE 5'-TGCTGACTCAGCA-3' DNA sequence (PubMed:23148532, PubMed:29339498).

Alternative names

Recommended products

Rabbit Polyclonal MAFA antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 18 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

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Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

MafA also known as musculoaponeurotic fibrosarcoma oncogene homolog A is a transcription factor with a molecular weight of approximately 43 kDa. This protein is expressed mainly in the pancreas particularly in the beta cells of the islets of Langerhans. MafA plays an important role in regulating insulin expression and secretion. High expression of MafA occurs during late-stage beta cell development making it an important marker for mature beta cells. Researchers often study MafA using techniques such as frozen tissue examination and flow cytometry with antibodies against MafA being important tools in these applications.

Biological function summary

MafA controls insulin gene transcription and supports beta cell function and survival. MafA does not function alone; it often forms complexes with other transcription factors like Pdx1 and NeuroD1 facilitating a coordinated regulation of insulin production and secretion. Its activity importantly aligns with the regulation of glucose metabolism responding to changes in glucose levels to ensure proper insulin release from the pancreas. In addition to its standalone role MafA synergizes with other transcription factors to modulate the expression of genes important to the maintenance of beta cell identity and function.

Pathways

The expression and function of MafA are integral to the insulin secretion pathway and the glucose homeostasis pathway. MafA operates alongside proteins like Pdx1 which also regulates insulin gene expression. The interplay between MafA and Pdx1 ensures appropriate response and adaptation of pancreatic beta cells to metabolic demands. Additionally the Wnt signaling pathway may intersect with MafA activity influencing beta cell proliferation and survival. Understanding these pathways is key to grappling with their impact on metabolic health and disease.

Associated diseases and disorders

MafA closely relates to diabetes mellitus particularly type 2 diabetes. Dysfunction or altered expression of MafA impairs insulin production contributing significantly to the pathogenesis of this disorder. Another protein Nkx6.1 often shows disrupted expression alongside MafA in diabetic conditions exacerbating issues in insulin regulation. Furthermore MafA mutations or misregulation may also contribute to gestational diabetes where its interaction with other transcription factors becomes impaired affecting insulin secretion during pregnancy. Researchers continue to study these relationships to develop therapeutic strategies targeting MafA for diabetes management.

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3 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MafA antibody (ab26405), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MafA antibody (ab26405)

    The image shows human pancreas tissue in which some cells exhibit nuclear staining. The antigen retrieval method used was Tris EDTA pH9.

  • Western blot - Anti-MafA antibody (ab26405), expandable thumbnail

    Western blot - Anti-MafA antibody (ab26405)

    Human MAFA has a predicted molecular weight of 37 kDa (Swiss-Prot data). Some publications suggest that the human protein should be detected at 47 kDa (PMID 12011435). Our data shows that ab26405 detects a band at 36 kDa in human brain, which is similar to that seen in other publications [PMID:14680841, PMID:18159220 (expression profile)]. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

    All lanes: Western blot - Anti-MafA antibody (ab26405) at 1 µg/mL

    All lanes: Brain (Human) Tissue Lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size: 36 kDa

    Observed band size: 36 kDa

  • Western blot - Anti-MafA antibody (ab26405), expandable thumbnail

    Image collected and cropped by CiteAb under a CC-BY license from the publication

    Western blot - Anti-MafA antibody (ab26405)

    MafA western blot using anti-MafA antibody ab26405. Publication image and figure legend from Bonnavion, R., Jaafar, R., et al., 2013, PLoS One, PubMed 24013263.

    ab26405 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab26405 please see the product overview.

    MAFA is detected in both human pancreatic alpha and beta cells.(A) Detection of ectopically expressed human MAFA and MAFB by western blot. The specificity of selected antibodies against human MAFA and MAFB were evaluated using mouse embryonic fibroblasts (MEF), which do not express endogenous murine MafA and MafB proteins, transfected respectively with constructs expressing human MAFA or MAFB protein by western-blot analysis. Protein extracts from MEF transfected with empty pcDNA and pcDNA expressing respectively human MAFA and MAFB were used for the detection, using antibodies against MAFA (Abcam) or MAFB (anti hMAFB2, mouse monoclonal antibodies, clone 1F4). Note that the anti-MAFA antibody and the noncommercial anti-hMAFB2 reacted specifically without cross-reaction, whereas the other tested commercially available anti-MAFB antibodies failed (data not shown) (B) Triple immunofluorescent (IF) staining showing MAFA expression in human islets from healthy donors. (C) The percentages of cells expressing MAFA were 88.3±6.3% MAFA+ beta cells and 61.2±6.4% MAFA+ alpha cells. Results are the averaged expression ±S.E.M of counting results from n = 4 control individuals (1058 INS+ cells and 345 GLU+ cells were counted in total). (D) ARX expression was detected in human pancreatic alpha cells but not human pancreatic beta cells. Representative images of triple IF-staining showing ARX expression in human islets from healthy donors. ARX was detected only in nuclei of alpha cells. (E) Co-localisation of MAFA with ARX in human islets. Right panels are the amplified view of the inset in the left panel. Red arrowheads, MAFA+ARX−GLU−cells. Green arrowheads, MAFA−ARX+GLU+. White arrowheads, MAFA+ARX+GLU+ cells. Scale bar  = 25 µM.

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