Anti-MafA antibody

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MafA antibody (AB26405)

The image shows human pancreas tissue in which some cells exhibit nuclear staining. The antigen retrieval method used was Tris EDTA pH9.

• WB

Project

Western blot - Anti-MafA antibody (AB26405)

Human MAFA has a predicted molecular weight of 37 kDa (Swiss-Prot data). Some publications suggest that the human protein should be detected at 47 kDa (PMID 12011435). Our data shows that ab26405 detects a band at 36 kDa in human brain, which is similar to that seen in other publications [PMID : 14680841, PMID : 18159220 (expression profile)]. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

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Western blot - Anti-MafA antibody (ab26405) at 1 µg/mL

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Brain (Human) Tissue Lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 36 kDa

Observed band size: 36 kDa

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• WB

CiteAb

Western blot - Anti-MafA antibody (AB26405)

MafA western blot using anti-MafA antibody ab26405. Publication image and figure legend from Bonnavion, R., Jaafar, R., et al., 2013, PLoS One, PubMed 24013263.

ab26405 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab26405 please see the product overview.

MAFA is detected in both human pancreatic alpha and beta cells.(A) Detection of ectopically expressed human MAFA and MAFB by western blot. The specificity of selected antibodies against human MAFA and MAFB were evaluated using mouse embryonic fibroblasts (MEF), which do not express endogenous murine MafA and MafB proteins, transfected respectively with constructs expressing human MAFA or MAFB protein by western-blot analysis. Protein extracts from MEF transfected with empty pcDNA and pcDNA expressing respectively human MAFA and MAFB were used for the detection, using antibodies against MAFA (Abcam) or MAFB (anti hMAFB2, mouse monoclonal antibodies, clone 1F4). Note that the anti-MAFA antibody and the noncommercial anti-hMAFB2 reacted specifically without cross-reaction, whereas the other tested commercially available anti-MAFB antibodies failed (data not shown) (B) Triple immunofluorescent (IF) staining showing MAFA expression in human islets from healthy donors. (C) The percentages of cells expressing MAFA were 88.3±6.3% MAFA+ beta cells and 61.2±6.4% MAFA+ alpha cells. Results are the averaged expression ±S.E.M of counting results from n = 4 control individuals (1058 INS+ cells and 345 GLU+ cells were counted in total). (D) ARX expression was detected in human pancreatic alpha cells but not human pancreatic beta cells. Representative images of triple IF-staining showing ARX expression in human islets from healthy donors. ARX was detected only in nuclei of alpha cells. (E) Co-localisation of MAFA with ARX in human islets. Right panels are the amplified view of the inset in the left panel. Red arrowheads, MAFA+ARX−GLU−cells. Green arrowheads, MAFA−ARX+GLU+. White arrowheads, MAFA+ARX+GLU+ cells. Scale bar  = 25 µM.

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