Rabbit Polyclonal MAFA antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 18 publications.
IgG
Rabbit
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Liquid
Polyclonal
WB | IHC-P | ICC/IF | |
---|---|---|---|
Human | Tested | Tested | Expected |
Mouse | Predicted | Predicted | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1 µg/mL | Notes Human MAFA has a predicted molecular weight of 37 kDa (Swiss-Prot data), however publications have shown the human protein to be detected at 47 kDa according to reference PMID 12011435, http://www.pnas.org/cgi/content/full/99/10/6737. Our data shows ab26405 to detect a band at 36 kDa in human brain, kidney and liver which is similar to that seen in other publications (PMID:14680841, PMID:18159220 (expression profile) ). The mouse protein has also been observed at 47 kDa according to reference PMID 12368292. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 0.8-4 µg/mL | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info - | Notes - |
Select an associated product type
Transcription factor that activates insulin gene expression (PubMed:12011435, PubMed:15993959). Acts synergistically with NEUROD1/BETA2 and PDX1 (PubMed:15993959). Binds the insulin enhancer C1/RIPE3b element (PubMed:12011435). Binds to consensus TRE-type MARE 5'-TGCTGACTCAGCA-3' DNA sequence (PubMed:23148532, PubMed:29339498).
Transcription factor MafA, Pancreatic beta-cell-specific transcriptional activator, RIPE3b1 factor, V-maf musculoaponeurotic fibrosarcoma oncogene homolog A, MAFA
Rabbit Polyclonal MAFA antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 18 publications.
IgG
Rabbit
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Liquid
Polyclonal
Affinity purification Immunogen
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
This supplementary information is collated from multiple sources and compiled automatically.
MafA also known as musculoaponeurotic fibrosarcoma oncogene homolog A is a transcription factor with a molecular weight of approximately 43 kDa. This protein is expressed mainly in the pancreas particularly in the beta cells of the islets of Langerhans. MafA plays an important role in regulating insulin expression and secretion. High expression of MafA occurs during late-stage beta cell development making it an important marker for mature beta cells. Researchers often study MafA using techniques such as frozen tissue examination and flow cytometry with antibodies against MafA being important tools in these applications.
MafA controls insulin gene transcription and supports beta cell function and survival. MafA does not function alone; it often forms complexes with other transcription factors like Pdx1 and NeuroD1 facilitating a coordinated regulation of insulin production and secretion. Its activity importantly aligns with the regulation of glucose metabolism responding to changes in glucose levels to ensure proper insulin release from the pancreas. In addition to its standalone role MafA synergizes with other transcription factors to modulate the expression of genes important to the maintenance of beta cell identity and function.
The expression and function of MafA are integral to the insulin secretion pathway and the glucose homeostasis pathway. MafA operates alongside proteins like Pdx1 which also regulates insulin gene expression. The interplay between MafA and Pdx1 ensures appropriate response and adaptation of pancreatic beta cells to metabolic demands. Additionally the Wnt signaling pathway may intersect with MafA activity influencing beta cell proliferation and survival. Understanding these pathways is key to grappling with their impact on metabolic health and disease.
MafA closely relates to diabetes mellitus particularly type 2 diabetes. Dysfunction or altered expression of MafA impairs insulin production contributing significantly to the pathogenesis of this disorder. Another protein Nkx6.1 often shows disrupted expression alongside MafA in diabetic conditions exacerbating issues in insulin regulation. Furthermore MafA mutations or misregulation may also contribute to gestational diabetes where its interaction with other transcription factors becomes impaired affecting insulin secretion during pregnancy. Researchers continue to study these relationships to develop therapeutic strategies targeting MafA for diabetes management.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
The image shows human pancreas tissue in which some cells exhibit nuclear staining. The antigen retrieval method used was Tris EDTA pH9.
Human MAFA has a predicted molecular weight of 37 kDa (Swiss-Prot data). Some publications suggest that the human protein should be detected at 47 kDa (PMID 12011435). Our data shows that ab26405 detects a band at 36 kDa in human brain, which is similar to that seen in other publications [PMID:14680841, PMID:18159220 (expression profile)]. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
All lanes: Western blot - Anti-MafA antibody (ab26405) at 1 µg/mL
All lanes: Brain (Human) Tissue Lysate at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 36 kDa
Observed band size: 36 kDa
Image collected and cropped by CiteAb under a CC-BY license from the publication
MafA western blot using anti-MafA antibody ab26405. Publication image and figure legend from Bonnavion, R., Jaafar, R., et al., 2013, PLoS One, PubMed 24013263.
ab26405 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab26405 please see the product overview.
MAFA is detected in both human pancreatic alpha and beta cells.(A) Detection of ectopically expressed human MAFA and MAFB by western blot. The specificity of selected antibodies against human MAFA and MAFB were evaluated using mouse embryonic fibroblasts (MEF), which do not express endogenous murine MafA and MafB proteins, transfected respectively with constructs expressing human MAFA or MAFB protein by western-blot analysis. Protein extracts from MEF transfected with empty pcDNA and pcDNA expressing respectively human MAFA and MAFB were used for the detection, using antibodies against MAFA (Abcam) or MAFB (anti hMAFB2, mouse monoclonal antibodies, clone 1F4). Note that the anti-MAFA antibody and the noncommercial anti-hMAFB2 reacted specifically without cross-reaction, whereas the other tested commercially available anti-MAFB antibodies failed (data not shown) (B) Triple immunofluorescent (IF) staining showing MAFA expression in human islets from healthy donors. (C) The percentages of cells expressing MAFA were 88.3±6.3% MAFA+ beta cells and 61.2±6.4% MAFA+ alpha cells. Results are the averaged expression ±S.E.M of counting results from n = 4 control individuals (1058 INS+ cells and 345 GLU+ cells were counted in total). (D) ARX expression was detected in human pancreatic alpha cells but not human pancreatic beta cells. Representative images of triple IF-staining showing ARX expression in human islets from healthy donors. ARX was detected only in nuclei of alpha cells. (E) Co-localisation of MAFA with ARX in human islets. Right panels are the amplified view of the inset in the left panel. Red arrowheads, MAFA+ARX−GLU−cells. Green arrowheads, MAFA−ARX+GLU+. White arrowheads, MAFA+ARX+GLU+ cells. Scale bar = 25 µM.
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com