Rabbit Recombinant Monoclonal MAFA antibody. Suitable for IHC-P, ICC, WB, ICC/IF and reacts with Mouse, Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human MAFA aa 100-200.
Images
![Western blot - Anti-MafA antibody [BLR067G] - BSA free (AB264418), expandable thumbnail](https://content.abcam.com/products/images/mafa-antibody-blr067g-bsa-free-ab264418--western-blot-img61491.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-MafA antibody [BLR067G] - BSA free (AB264418)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MafA antibody [BLR067G] - BSA free (AB264418), expandable thumbnail](https://content.abcam.com/products/images/mafa-antibody-blr067g-bsa-free-ab264418--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img58011.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MafA antibody [BLR067G] - BSA free (AB264418)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MafA antibody [BLR067G] - BSA free (AB264418), expandable thumbnail](https://content.abcam.com/products/images/mafa-antibody-blr067g-bsa-free-ab264418--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img24471.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MafA antibody [BLR067G] - BSA free (AB264418)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MafA antibody [BLR067G] - BSA free (AB264418), expandable thumbnail](https://content.abcam.com/products/images/mafa-antibody-blr067g-bsa-free-ab264418--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img132929.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MafA antibody [BLR067G] - BSA free (AB264418)")
![Immunocytochemistry/ Immunofluorescence - Anti-MafA antibody [BLR067G] - BSA free (AB264418), expandable thumbnail](https://content.abcam.com/products/images/mafa-antibody-blr067g-bsa-free-ab264418--immunocytochemistry-immunofluorescence-img127305.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-MafA antibody [BLR067G] - BSA free (AB264418)")
Publications
Filter by
- Metabolites 13:2023Expression Silencing of Mitogen-Activated Protein Kinase 8 Interacting Protein-1 Conferred Its Role in Pancreatic β-Cell Physiology and Insulin Secretion.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRania Saeed,Abdul Khader Mohammed,Sarra E Saleh,Khaled M Aboshanab,Mohammad M Aboulwafa,Jalal TaneeraPubMed 36837926
- Experimental biology and medicine (Maywood, N.J.) 248:339-3492023plays a critical role in insulin secretion in INS-1 cells and human pancreatic islets.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMohammad G Mohammad,Rania Saeed,Abdul Khader Mohammed,Anila Khalique,Mohamad Hamad,Waseem El-Huneidi,Mawieh Hamad,Jalal TaneeraPubMed 36740767
- Frontiers in oncology 12:8715682022Integrative Proteo-Genomic Analysis for Recurrent Survival Prognosis in Colon Adenocarcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFeiYan Ai,Wenhao Wang,Shaojun Liu,Decai Zhang,Zhenyu Yang,Fen LiuPubMed 35847888
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 99% Borate buffered saline- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Synthetic Peptide within Human MAFA aa 100-200. The exact immunogen used to generate this antibody is proprietary information. Database link Q8NHW3
Reactivity data
Select an application| IHC-P | ICC | WB | ICC/IF | |
|---|---|---|---|---|
| Human | Tested | Expected | Tested | Expected |
| Mouse | Tested | Tested | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100 - 1/500 | Notes Zinc-fixative (JB fix) is recommended to enhance staining. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/100 - 1/500 | Notes Zinc-fixative (JB fix) is recommended to enhance staining. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100 - 1/500 | Notes Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes - |
Species Human | Dilution info 1/1000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100 - 1/500 | Notes Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
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Target data
Function
Transcription factor that activates insulin gene expression (PubMed:12011435, PubMed:15993959). Acts synergistically with NEUROD1/BETA2 and PDX1 (PubMed:15993959). Binds the insulin enhancer C1/RIPE3b element (PubMed:12011435). Binds to consensus TRE-type MARE 5'-TGCTGACTCAGCA-3' DNA sequence (PubMed:23148532, PubMed:29339498).
Alternative names
Transcription factor MafA, Pancreatic beta-cell-specific transcriptional activator, RIPE3b1 factor, V-maf musculoaponeurotic fibrosarcoma oncogene homolog A, MAFA
Recommended products
Rabbit Recombinant Monoclonal MAFA antibody. Suitable for IHC-P, ICC, WB, ICC/IF and reacts with Mouse, Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human MAFA aa 100-200.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 99% Borate buffered saline- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Synthetic Peptide within Human MAFA aa 100-200. The exact immunogen used to generate this antibody is proprietary information. Database link Q8NHW3
- Clone number
- BLR067G
- Concentration
- Purification notes
Purified from cell culture supernatant.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product is sold under License from Bethyl Laboratories, Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MafA also known as musculoaponeurotic fibrosarcoma oncogene homolog A is a transcription factor with a molecular weight of approximately 43 kDa. This protein is expressed mainly in the pancreas particularly in the beta cells of the islets of Langerhans. MafA plays an important role in regulating insulin expression and secretion. High expression of MafA occurs during late-stage beta cell development making it an important marker for mature beta cells. Researchers often study MafA using techniques such as frozen tissue examination and flow cytometry with antibodies against MafA being important tools in these applications.
- Biological function summary
MafA controls insulin gene transcription and supports beta cell function and survival. MafA does not function alone; it often forms complexes with other transcription factors like Pdx1 and NeuroD1 facilitating a coordinated regulation of insulin production and secretion. Its activity importantly aligns with the regulation of glucose metabolism responding to changes in glucose levels to ensure proper insulin release from the pancreas. In addition to its standalone role MafA synergizes with other transcription factors to modulate the expression of genes important to the maintenance of beta cell identity and function.
- Pathways
The expression and function of MafA are integral to the insulin secretion pathway and the glucose homeostasis pathway. MafA operates alongside proteins like Pdx1 which also regulates insulin gene expression. The interplay between MafA and Pdx1 ensures appropriate response and adaptation of pancreatic beta cells to metabolic demands. Additionally the Wnt signaling pathway may intersect with MafA activity influencing beta cell proliferation and survival. Understanding these pathways is key to grappling with their impact on metabolic health and disease.
- Associated diseases and disorders
MafA closely relates to diabetes mellitus particularly type 2 diabetes. Dysfunction or altered expression of MafA impairs insulin production contributing significantly to the pathogenesis of this disorder. Another protein Nkx6.1 often shows disrupted expression alongside MafA in diabetic conditions exacerbating issues in insulin regulation. Furthermore MafA mutations or misregulation may also contribute to gestational diabetes where its interaction with other transcription factors becomes impaired affecting insulin secretion during pregnancy. Researchers continue to study these relationships to develop therapeutic strategies targeting MafA for diabetes management.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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7 product images
![Western blot - Anti-MafA antibody [BLR067G] - BSA free (ab264418), expandable thumbnail](https://content.abcam.com/products/images/mafa-antibody-blr067g-bsa-free-ab264418--western-blot-img61491.jpg "Western blot - Anti-MafA antibody [BLR067G] - BSA free (ab264418)")
Western blot - Anti-MafA antibody [BLR067G] - BSA free (ab264418)
All lanes: Western blot - Anti-MafA antibody [BLR067G] - BSA free (ab264418) at 1/1000 dilution
Lane 1: Beta-TC-6 whole cell lysate at 10 µg
Lane 2: AlphaTC1 clone 9 whole cell lysate at 50 µg
Lane 3: NIH/3T3 whole cell lysate at 50 µg
SecondaryAll lanes: HRP-conjugated goat anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 36 kDa
Exposure time: 3min
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MafA antibody [BLR067G] - BSA free (ab264418), expandable thumbnail](https://content.abcam.com/products/images/mafa-antibody-blr067g-bsa-free-ab264418--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img58011.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MafA antibody [BLR067G] - BSA free (ab264418)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MafA antibody [BLR067G] - BSA free (ab264418)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human pancreatic carcinoma tissue labeling MafA with ab264418 at 1/250 dilution. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: Opal™. Counterstain: DAPI (blue).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MafA antibody [BLR067G] - BSA free (ab264418), expandable thumbnail](https://content.abcam.com/products/images/mafa-antibody-blr067g-bsa-free-ab264418--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img24471.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MafA antibody [BLR067G] - BSA free (ab264418)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MafA antibody [BLR067G] - BSA free (ab264418)
Immunohistochemical analysis of JB zinc-fixed, paraffin-embedded mouse pancreas tissue labeling MafA with ab264418 at 1/200 dilution. Detection: DAB.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MafA antibody [BLR067G] - BSA free (ab264418), expandable thumbnail](https://content.abcam.com/products/images/mafa-antibody-blr067g-bsa-free-ab264418--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img132929.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MafA antibody [BLR067G] - BSA free (ab264418)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MafA antibody [BLR067G] - BSA free (ab264418)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human pancreatic tumor tissue labeling MafA with ab264418 at 1/200 dilution. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
![Immunocytochemistry/ Immunofluorescence - Anti-MafA antibody [BLR067G] - BSA free (ab264418), expandable thumbnail](https://content.abcam.com/products/images/mafa-antibody-blr067g-bsa-free-ab264418--immunocytochemistry-immunofluorescence-img127305.jpg "Immunocytochemistry/ Immunofluorescence - Anti-MafA antibody [BLR067G] - BSA free (ab264418)")
Immunocytochemistry/ Immunofluorescence - Anti-MafA antibody [BLR067G] - BSA free (ab264418)
Immunocytochemical analysis of formalin-fixed, paraffin-embedded mouse beta-TC-6 cells labeling MafA with ab264418 at 1/200 dilution. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MafA antibody [BLR067G] - BSA free (ab264418), expandable thumbnail](https://content.abcam.com/products/images/mafa-antibody-blr067g-bsa-free-ab264418--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img106224.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MafA antibody [BLR067G] - BSA free (ab264418)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MafA antibody [BLR067G] - BSA free (ab264418)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human pancreas tissue labeling MafA with ab264418 at 1/200 dilution. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
![Western blot - Anti-MafA antibody [BLR067G] - BSA free (ab264418), expandable thumbnail](https://content.abcam.com/products/images/mafa-antibody-blr067g-bsa-free-ab264418--western-blot-img108153.jpg "Western blot - Anti-MafA antibody [BLR067G] - BSA free (ab264418)")
Western blot - Anti-MafA antibody [BLR067G] - BSA free (ab264418)
Lysates prepared using NETN lysis buffer.
All lanes: Western blot - Anti-MafA antibody [BLR067G] - BSA free (ab264418) at 1/1000 dilution
Lane 1: Whole cell lysate from HEK-293T transfected with human MafA
Lane 2: Whole cell lysate from HEK-293T transfected with human MafB
Lane 3: Whole cell lysate from HEK-293T transfected with human MafC
Lane 4: Whole cell lysate from HEK-293T transfected with mouse MafA
Lane 5: Whole cell lysate from HEK-293T transfected with mouse MafB
Lane 6: Whole cell lysate from HEK-293T transfected with mouse MafC
SecondaryAll lanes: HRP-conjugated goat anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 36 kDa
Exposure time: 30s
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MafA antibody [BLR067G] - BSA free (ab264418), expandable thumbnail](https://content.abcam.com/products/images/mafa-antibody-blr067g-bsa-free-ab264418--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img106224.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MafA antibody [BLR067G] - BSA free (ab264418)")
![Western blot - Anti-MafA antibody [BLR067G] - BSA free (ab264418), expandable thumbnail](https://content.abcam.com/products/images/mafa-antibody-blr067g-bsa-free-ab264418--western-blot-img108153.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-MafA antibody [BLR067G] - BSA free (ab264418)")
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