Rabbit Polyclonal MAFB antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MAFB aa 1-150.
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- Nature :2023A human embryonic limb cell atlas resolved in space and time.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBao Zhang,Peng He,John E G Lawrence,Shuaiyu Wang,Elizabeth Tuck,Brian A Williams,Kenny Roberts,Vitalii Kleshchevnikov,Lira Mamanova,Liam Bolt,Krzysztof Polanski,Tong Li,Rasa Elmentaite,Eirini S Fasouli,Martin Prete,Xiaoling He,Nadav Yayon,Yixi Fu,Hao Yang,Chen Liang,Hui Zhang,Raphael Blain,Alain Chedotal,David R FitzPatrick,Helen Firth,Andrew Dean,Omer Ali Bayraktar,John C Marioni,Roger A Barker,Mekayla A Storer,Barbara J Wold,Hongbo Zhang,Sarah A TeichmannPubMed 38057666
- Stem cell research & therapy 13:3552022Podocytes derived from human induced pluripotent stem cells: characterization, comparison, and modeling of diabetic kidney disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJulie Bejoy,Justin M Farry,Jennifer L Peek,Mariana C Cabatu,Felisha M Williams,Richard C Welch,Eddie S Qian,Lauren E WoodardPubMed 35883199
- STAR protocols 2:1008982021Accelerated protocol for the differentiation of podocytes from human pluripotent stem cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJulie Bejoy,Eddie Spencer Qian,Lauren Elizabeth WoodardPubMed 34746862
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human MAFB aa 1-150. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y5Q3
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Reactivity data
Select an application| IHC-P | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
| Cynomolgus monkey | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200 - 1/500 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cynomolgus monkey | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25-2 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cynomolgus monkey | Dilution info - | Notes - |
Target data
Function
Acts as a transcriptional activator or repressor (PubMed:27181683). Plays a pivotal role in regulating lineage-specific hematopoiesis by repressing ETS1-mediated transcription of erythroid-specific genes in myeloid cells. Required for monocytic, macrophage, osteoclast, podocyte and islet beta cell differentiation. Involved in renal tubule survival and F4/80 maturation. Activates the insulin and glucagon promoters. Together with PAX6, transactivates weakly the glucagon gene promoter through the G1 element. SUMO modification controls its transcriptional activity and ability to specify macrophage fate. Binds element G1 on the glucagon promoter (By similarity). Involved either as an oncogene or as a tumor suppressor, depending on the cell context. Required for the transcriptional activation of HOXB3 in the rhombomere r5 in the hindbrain (By similarity).
Alternative names
KRML, MAFB, Transcription factor MafB, Maf-B, V-maf musculoaponeurotic fibrosarcoma oncogene homolog B
Recommended products
Rabbit Polyclonal MAFB antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MAFB aa 1-150.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human MAFB aa 1-150. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y5Q3
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MafB also known as V-maf musculoaponeurotic fibrosarcoma oncogene homolog B acts as a transcription factor involved in the regulation of gene expression. The protein has a molecular weight of approximately 37 kDa. MafB localizes predominantly to the nucleus where it exerts its function. Expression of MafB occurs in various tissues with significant levels in hematopoietic cells kidney and pancreas reflecting its role in cell differentiation and organ development.
- Biological function summary
MafB plays an important role in the regulation of monocyte and macrophage differentiation. It serves as an important regulator that controls genes required for the immune response and terminal differentiation of these cells. MafB often operates as a heterodimer pairing with other transcription factors like Fos and Jun to form complexes that specifically bind to Maf recognition elements in DNA. This binding allows it to orchestrate various transcriptional programs necessary for immune system function and organogenesis.
- Pathways
MafB integrates into the larger network of macrophage differentiation and the insulin signaling pathway. It interacts closely with proteins like PU.1 in the macrophage differentiation pathway where it helps specify the lineage commitment of hematopoietic progenitor cells. In the insulin signaling pathway MafB influences genes involved in pancreatic beta-cell function and glucose homeostasis showing its interconnected role in metabolic processes.
- Associated diseases and disorders
MafB connects to conditions such as diabetes and systemic lupus erythematosus (SLE). In diabetes MafB affects insulin secretion by regulating pancreatic beta-cell development and function and studies link its dysfunction to both Type 1 and Type 2 diabetes. In SLE aberrant expression of MafB possibly associates with autoimmunity where it might alter the normal immune response. Proteins like IL-6 involved in inflammation and immune response often interact with MafB highlighting the protein's significance in disease contexts.
Product promise
Tested
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Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MafB antibody (ab223744)
Paraffin-embedded human testis tissue stained for MafB using ab223744 at 1/200 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence - Anti-MafB antibody (ab223744)
PFA-fixed, Triton X-100 permeabilized SiHa cells stained for MafB (green) using ab223744 at 4 μg/ml in ICC/IF.
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