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AB223744

Anti-MafB antibody

4

(3 Reviews)

|

(5 Publications)

Rabbit Polyclonal MAFB antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MAFB aa 1-150.

View Alternative Names

KRML, MAFB, Transcription factor MafB, Maf-B, V-maf musculoaponeurotic fibrosarcoma oncogene homolog B

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MafB antibody (AB223744)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MafB antibody (AB223744)

Paraffin-embedded human testis tissue stained for MafB using ab223744 at 1/200 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-MafB antibody (AB223744)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MafB antibody (AB223744)

PFA-fixed, Triton X-100 permeabilized SiHa cells stained for MafB (green) using ab223744 at 4 μg/ml in ICC/IF.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human MAFB aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

Q9Y5Q3

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cynomolgus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MafB also known as V-maf musculoaponeurotic fibrosarcoma oncogene homolog B acts as a transcription factor involved in the regulation of gene expression. The protein has a molecular weight of approximately 37 kDa. MafB localizes predominantly to the nucleus where it exerts its function. Expression of MafB occurs in various tissues with significant levels in hematopoietic cells kidney and pancreas reflecting its role in cell differentiation and organ development.
Biological function summary

MafB plays an important role in the regulation of monocyte and macrophage differentiation. It serves as an important regulator that controls genes required for the immune response and terminal differentiation of these cells. MafB often operates as a heterodimer pairing with other transcription factors like Fos and Jun to form complexes that specifically bind to Maf recognition elements in DNA. This binding allows it to orchestrate various transcriptional programs necessary for immune system function and organogenesis.

Pathways

MafB integrates into the larger network of macrophage differentiation and the insulin signaling pathway. It interacts closely with proteins like PU.1 in the macrophage differentiation pathway where it helps specify the lineage commitment of hematopoietic progenitor cells. In the insulin signaling pathway MafB influences genes involved in pancreatic beta-cell function and glucose homeostasis showing its interconnected role in metabolic processes.

MafB connects to conditions such as diabetes and systemic lupus erythematosus (SLE). In diabetes MafB affects insulin secretion by regulating pancreatic beta-cell development and function and studies link its dysfunction to both Type 1 and Type 2 diabetes. In SLE aberrant expression of MafB possibly associates with autoimmunity where it might alter the normal immune response. Proteins like IL-6 involved in inflammation and immune response often interact with MafB highlighting the protein's significance in disease contexts.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Acts as a transcriptional activator or repressor (PubMed : 27181683). Plays a pivotal role in regulating lineage-specific hematopoiesis by repressing ETS1-mediated transcription of erythroid-specific genes in myeloid cells. Required for monocytic, macrophage, osteoclast, podocyte and islet beta cell differentiation. Involved in renal tubule survival and F4/80 maturation. Activates the insulin and glucagon promoters. Together with PAX6, transactivates weakly the glucagon gene promoter through the G1 element. SUMO modification controls its transcriptional activity and ability to specify macrophage fate. Binds element G1 on the glucagon promoter (By similarity). Involved either as an oncogene or as a tumor suppressor, depending on the cell context. Required for the transcriptional activation of HOXB3 in the rhombomere r5 in the hindbrain (By similarity).
See full target information MAFB
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Nature : PubMed38057666

2023

A human embryonic limb cell atlas resolved in space and time.

Applications

Unspecified application

Species

Unspecified reactive species

Bao Zhang,Peng He,John E G Lawrence,Shuaiyu Wang,Elizabeth Tuck,Brian A Williams,Kenny Roberts,Vitalii Kleshchevnikov,Lira Mamanova,Liam Bolt,Krzysztof Polanski,Tong Li,Rasa Elmentaite,Eirini S Fasouli,Martin Prete,Xiaoling He,Nadav Yayon,Yixi Fu,Hao Yang,Chen Liang,Hui Zhang,Raphael Blain,Alain Chedotal,David R FitzPatrick,Helen Firth,Andrew Dean,Omer Ali Bayraktar,John C Marioni,Roger A Barker,Mekayla A Storer,Barbara J Wold,Hongbo Zhang,Sarah A Teichmann

Immunity 56:2152-2171.e13 PubMed37582369

2023

Human microglia maturation is underpinned by specific gene regulatory networks.

Applications

Unspecified application

Species

Unspecified reactive species

Claudia Z Han,Rick Z Li,Emily Hansen,Samantha Trescott,Bethany R Fixsen,Celina T Nguyen,Cristina M Mora,Nathanael J Spann,Hunter R Bennett,Olivier Poirion,Justin Buchanan,Anna S Warden,Bing Xia,Johannes C M Schlachetzki,Martina P Pasillas,Sebastian Preissl,Allen Wang,Carolyn O'Connor,Shreya Shriram,Roy Kim,Danielle Schafer,Gabriela Ramirez,Jean Challacombe,Samuel A Anavim,Avalon Johnson,Mihir Gupta,Ian A Glass,Michael L Levy,Sharona Ben Haim,David D Gonda,Louise Laurent,Jennifer F Hughes,David C Page,Mathew Blurton-Jones,Christopher K Glass,Nicole G Coufal

Stem cell research & therapy 13:355 PubMed35883199

2022

Podocytes derived from human induced pluripotent stem cells: characterization, comparison, and modeling of diabetic kidney disease.

Applications

Unspecified application

Species

Unspecified reactive species

Julie Bejoy,Justin M Farry,Jennifer L Peek,Mariana C Cabatu,Felisha M Williams,Richard C Welch,Eddie S Qian,Lauren E Woodard

STAR protocols 2:100898 PubMed34746862

2021

Accelerated protocol for the differentiation of podocytes from human pluripotent stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Julie Bejoy,Eddie Spencer Qian,Lauren Elizabeth Woodard

Nature communications 12:1453 PubMed33674603

2021

Identification of CD318, TSPAN8 and CD66c as target candidates for CAR T cell based immunotherapy of pancreatic adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel Schäfer,Stefan Tomiuk,Laura N Küster,Wa'el Al Rawashdeh,Janina Henze,German Tischler-Höhle,David J Agorku,Janina Brauner,Cathrin Linnartz,Dominik Lock,Andrew Kaiser,Christoph Herbel,Dominik Eckardt,Melina Lamorte,Dorothee Lenhard,Julia Schüler,Philipp Ströbel,Jeannine Missbach-Guentner,Diana Pinkert-Leetsch,Frauke Alves,Andreas Bosio,Olaf Hardt
View all publications
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Product promise

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