AB243902

Anti-MafB antibody [BLR046F] - BSA free

Recombinant
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(2 Publications)

Rabbit Recombinant Monoclonal MAFB antibody. Suitable for IHC-P, ICC, IP, WB and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human MAFB aa 1-50.

View Alternative Names

KRML, MAFB, Transcription factor MafB, Maf-B, V-maf musculoaponeurotic fibrosarcoma oncogene homolog B

5 Images

Supplier Data

Immunocytochemistry - Anti-MafB antibody [BLR046F] - BSA free (AB243902)

Immunocytochemical analysis of formalin fixed paraffin embedded human SK-N-MC cells staining MafB with ab243902 and secondary HRP-conjugated goat anti-rabbit IgG

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MafB antibody [BLR046F] - BSA free (AB243902)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MafB antibody [BLR046F] - BSA free (AB243902)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pancreatic tumour tissue labelling MafB with ab243902 and secondary HRP conjugated goat anti-rabbit IgG. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Supplier Data

Immunoprecipitation - Anti-MafB antibody [BLR046F] - BSA free (AB243902)

MafB was immunoprecipitated from SK-N-MC whole cell lysate (1 mg per IP reaction, 20% loaded) with ab243902 at 6 μg per reaction. Western blot was performed on the immunoprecipitate using ab243902 at 1/1000 dilution.

Lane 1 : ab243902 IP in SK-N-MC whole cell lysate.

Lane 2 : ab243902 IP in SK-N-MC whole cell lysate

Lane 4 : Control IgG in HeLa whole cell lysate.

Detection : Chemiluminescence with an exposure time of 30 seconds

All lanes:

Immunoprecipitation - Anti-MafB antibody [BLR046F] - BSA free (ab243902)

Predicted band size: 36 kDa

false

Supplier Data

Western blot - Anti-MafB antibody [BLR046F] - BSA free (AB243902)

All lanes:

Western blot - Anti-MafB antibody [BLR046F] - BSA free (ab243902) at 1/1000 dilution

Lane 1:

Beta TC-6 whole cell lysate at 50 µg

Lane 2:

Alpha TC1 whole cell lysate at 50 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG

Predicted band size: 36 kDa

false

Supplier Data

Western blot - Anti-MafB antibody [BLR046F] - BSA free (AB243902)

All lanes:

Western blot - Anti-MafB antibody [BLR046F] - BSA free (ab243902) at 1/1000 dilution

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HEK293T whole cell lysate at 50 µg

Lane 3:

SK-N-MC whole cell lysate at 50 µg

Lane 4:

RPM-8226 whole cell lysate at 50 µg

Lane 5:

Jurkat whole cell lysate at 50 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG

Predicted band size: 36 kDa

false

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

BLR046F

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

ICC, IHC-P, IP, WB

applications

Immunogen

Synthetic Peptide within Human MAFB aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q9Y5Q3

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1/100 - 1/500", "ICC-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "Use 6μl/mg lysate.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" } } }

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Product details

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

This product is sold under License from Bethyl Laboratories, Inc.

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Properties and storage information

Form

Liquid

Purification notes

Recombinant antibody was purified from cell culture supernatant.

Storage buffer

pH: 7.8 - 8.6 Preservative: 0.09% Sodium azide Constituents: 99% Borate buffered saline

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MafB also known as V-maf musculoaponeurotic fibrosarcoma oncogene homolog B acts as a transcription factor involved in the regulation of gene expression. The protein has a molecular weight of approximately 37 kDa. MafB localizes predominantly to the nucleus where it exerts its function. Expression of MafB occurs in various tissues with significant levels in hematopoietic cells kidney and pancreas reflecting its role in cell differentiation and organ development.

Biological function summary

MafB plays an important role in the regulation of monocyte and macrophage differentiation. It serves as an important regulator that controls genes required for the immune response and terminal differentiation of these cells. MafB often operates as a heterodimer pairing with other transcription factors like Fos and Jun to form complexes that specifically bind to Maf recognition elements in DNA. This binding allows it to orchestrate various transcriptional programs necessary for immune system function and organogenesis.

Pathways

MafB integrates into the larger network of macrophage differentiation and the insulin signaling pathway. It interacts closely with proteins like PU.1 in the macrophage differentiation pathway where it helps specify the lineage commitment of hematopoietic progenitor cells. In the insulin signaling pathway MafB influences genes involved in pancreatic beta-cell function and glucose homeostasis showing its interconnected role in metabolic processes.

MafB connects to conditions such as diabetes and systemic lupus erythematosus (SLE). In diabetes MafB affects insulin secretion by regulating pancreatic beta-cell development and function and studies link its dysfunction to both Type 1 and Type 2 diabetes. In SLE aberrant expression of MafB possibly associates with autoimmunity where it might alter the normal immune response. Proteins like IL-6 involved in inflammation and immune response often interact with MafB highlighting the protein's significance in disease contexts.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Acts as a transcriptional activator or repressor (PubMed : 27181683). Plays a pivotal role in regulating lineage-specific hematopoiesis by repressing ETS1-mediated transcription of erythroid-specific genes in myeloid cells. Required for monocytic, macrophage, osteoclast, podocyte and islet beta cell differentiation. Involved in renal tubule survival and F4/80 maturation. Activates the insulin and glucagon promoters. Together with PAX6, transactivates weakly the glucagon gene promoter through the G1 element. SUMO modification controls its transcriptional activity and ability to specify macrophage fate. Binds element G1 on the glucagon promoter (By similarity). Involved either as an oncogene or as a tumor suppressor, depending on the cell context. Required for the transcriptional activation of HOXB3 in the rhombomere r5 in the hindbrain (By similarity).

See full target information MAFB

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Genes & development 38:738-754 PubMed39168636

2024

The SAGA acetyltransferase module is required for the maintenance of MAF and MYC oncogenic gene expression programs in multiple myeloma.

Applications

Unspecified application

Species

Unspecified reactive species

Ying-Jiun C Chen,Govinal Badiger Bhaskara,Yue Lu,Kevin Lin,Sharon Y R Dent

Aging 14:2793-2804 PubMed35333774

2022

Local anesthetic levobupivacaine inhibits stemness of osteosarcoma cells by epigenetically repressing MAFB though reducing KAT5 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Zhan Wang,Yuxin Song,Hui Zhang,Yang Yang,Suifeng Zhang,Wenji Wang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MafB antibody [BLR046F] - BSA free

Immunocytochemistry - Anti-MafB antibody [BLR046F] - BSA free (AB243902)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MafB antibody [BLR046F] - BSA free (AB243902)

Immunoprecipitation - Anti-MafB antibody [BLR046F] - BSA free (AB243902)

Western blot - Anti-MafB antibody [BLR046F] - BSA free (AB243902)

Western blot - Anti-MafB antibody [BLR046F] - BSA free (AB243902)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise