Rabbit Polyclonal MAFF antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human MAFF.
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- BMC cardiovascular disorders 21:4482021Proteome-scale profiling reveals MAFF and MAFG as two novel key transcription factors involved in palmitic acid-induced umbilical vein endothelial cell apoptosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMangyuan Wang,Fen Liu,Binbin Fang,Qiang Huo,Yining YangPubMed 34535081
- Oncology research 28:299-3092020MafF Is Regulated via the circ-ITCH/miR-224-5p Axis and Acts as a Tumor Suppressor in Hepatocellular Carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMinhua Wu et. al.PubMed 31969212
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human MAFF. Database link Q9ULX9
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 - 1/3000 | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Since they lack a putative transactivation domain, the small Mafs behave as transcriptional repressors when they dimerize among themselves (PubMed:8932385). However, they seem to serve as transcriptional activators by dimerizing with other (usually larger) basic-zipper proteins, such as NFE2L1/NRF1, and recruiting them to specific DNA-binding sites. Interacts with the upstream promoter region of the oxytocin receptor gene (PubMed:16549056, PubMed:8932385). May be a transcriptional enhancer in the up-regulation of the oxytocin receptor gene at parturition (PubMed:10527846).
Alternative names
Transcription factor MafF, U-Maf, V-maf musculoaponeurotic fibrosarcoma oncogene homolog F, MAFF
Recommended products
Rabbit Polyclonal MAFF antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human MAFF.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human MAFF. Database link Q9ULX9
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The protein MAFF also known as v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog F functions as a transcription factor. It is a small protein with a molecular mass of approximately 18 kDa. MAFF is expressed in various tissues including the brain liver and pancreas. It belongs to the small Maf family characterized by their lack of transcriptional activation domains necessitating dimerization with other proteins to regulate gene expression.
- Biological function summary
The small Maf protein MAFF plays an important role in regulating oxidative stress response and the coordinated expression of genes involved in detoxification processes. When part of a complex it often dimerizes with Nrf2 a protein that activates the expression of antioxidant response elements (ARE) in gene promoters. This kind of dimerization enables MAFF to indirectly regulate the transcription of genes involved in the cellular protection against oxidative damage.
- Pathways
MAFF integrates into the antioxidant defense mechanism especially through its interaction with the Nrf2-Keap1 signaling pathway. Within this pathway MAFF through its interaction with Nrf2 facilitates the induction of cytoprotective genes that help in detoxification and maintaining cellular redox homeostasis. The interaction between MAFF and Nrf2 is significant as Nrf2 becomes stabilized and translocates to the nucleus under oxidative stress which is key for the pathway's protective effects.
- Associated diseases and disorders
MAFF through its interaction mainly with Nrf2 is associated with conditions like cancer and neurodegenerative diseases. In cancer the deregulation of the Nrf2 pathway often involving MAFF can lead to resistance against chemotherapy due to enhanced cellular antioxidant defenses therefore promoting tumor survival. Additionally in neurodegenerative disorders the inefficient activation of the MAFF-Nrf2 pathway may contribute to the accumulation of oxidative damage an underlying factor in diseases like Alzheimer's.
Product promise
Tested
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Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-MAFF antibody (ab183859)
All lanes: Western blot - Anti-MAFF antibody (ab183859) at 1/2000 dilution
Lane 1: Huh7 whole cell lysate/extract at 30 µg
Lane 2: HepG2 whole cell lysate/extract at 30 µg
Predicted band size: 18 kDa
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