JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB198249

Anti-MAFF antibody

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Polyclonal MAFF antibody. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human MAFF.

View Alternative Names

Transcription factor MafF, U-Maf, V-maf musculoaponeurotic fibrosarcoma oncogene homolog F, MAFF

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAFF antibody (AB198249)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAFF antibody (AB198249)

Immunohistochemical analysis of paraffin-embedded Human esophagus cancer tissue labeling MAFF with ab198249 at 1/20 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAFF antibody (AB198249)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAFF antibody (AB198249)

Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue labeling MAFF with ab198249 at 1/20 dilution.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human MAFF. The exact immunogen used to generate this antibody is proprietary information.

Q9ULX9

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/100", "IHCP-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein MAFF also known as v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog F functions as a transcription factor. It is a small protein with a molecular mass of approximately 18 kDa. MAFF is expressed in various tissues including the brain liver and pancreas. It belongs to the small Maf family characterized by their lack of transcriptional activation domains necessitating dimerization with other proteins to regulate gene expression.
Biological function summary

The small Maf protein MAFF plays an important role in regulating oxidative stress response and the coordinated expression of genes involved in detoxification processes. When part of a complex it often dimerizes with Nrf2 a protein that activates the expression of antioxidant response elements (ARE) in gene promoters. This kind of dimerization enables MAFF to indirectly regulate the transcription of genes involved in the cellular protection against oxidative damage.

Pathways

MAFF integrates into the antioxidant defense mechanism especially through its interaction with the Nrf2-Keap1 signaling pathway. Within this pathway MAFF through its interaction with Nrf2 facilitates the induction of cytoprotective genes that help in detoxification and maintaining cellular redox homeostasis. The interaction between MAFF and Nrf2 is significant as Nrf2 becomes stabilized and translocates to the nucleus under oxidative stress which is key for the pathway's protective effects.

MAFF through its interaction mainly with Nrf2 is associated with conditions like cancer and neurodegenerative diseases. In cancer the deregulation of the Nrf2 pathway often involving MAFF can lead to resistance against chemotherapy due to enhanced cellular antioxidant defenses therefore promoting tumor survival. Additionally in neurodegenerative disorders the inefficient activation of the MAFF-Nrf2 pathway may contribute to the accumulation of oxidative damage an underlying factor in diseases like Alzheimer's.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Since they lack a putative transactivation domain, the small Mafs behave as transcriptional repressors when they dimerize among themselves (PubMed : 8932385). However, they seem to serve as transcriptional activators by dimerizing with other (usually larger) basic-zipper proteins, such as NFE2L1/NRF1, and recruiting them to specific DNA-binding sites. Interacts with the upstream promoter region of the oxytocin receptor gene (PubMed : 16549056, PubMed : 8932385). May be a transcriptional enhancer in the up-regulation of the oxytocin receptor gene at parturition (PubMed : 10527846).
See full target information MAFF
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Heliyon 10:e30603 PubMed38726149

2024

Tumor suppressor function of RBMS3 overexpression in EOC associated with immune cell infiltration.

Applications

Unspecified application

Species

Unspecified reactive species

Tian Yin,Ying Zhang,Yue Zhao,Xinyi Zhang,Shuqi Han,Yixiao Wang,Bo Yang
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB227721

Anti-MAFF antibody - N-terminal

Western blot - Anti-MAFF antibody - N-terminal (AB227721)

  • 0
  • 0
Primary Antibodies

AB183859

Anti-MAFF antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAFF antibody (AB183859)

  • 0
  • 0

Complementary Products

Proteins & Peptides

AB161761

Recombinant Human MAFF protein

SDS-PAGE - Recombinant Human MAFF protein (AB161761)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB32503

Anti-Bax antibody [E63]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [E63] (AB32503)

  • 4
  • 12
Primary Antibodies

AB182858

Anti-Bcl-2 antibody [EPR17509]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 antibody [EPR17509] (AB182858)

  • 5
  • 13

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com