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AB227721

Anti-MAFF antibody - N-terminal

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(3 Publications)

Rabbit Polyclonal MAFF antibody. N-terminal. Suitable for WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MAFF.

View Alternative Names

Transcription factor MafF, U-Maf, V-maf musculoaponeurotic fibrosarcoma oncogene homolog F, MAFF

1 Images
Western blot - Anti-MAFF antibody - N-terminal (AB227721)
  • WB

Supplier Data

Western blot - Anti-MAFF antibody - N-terminal (AB227721)

12% SDS-PAGE

All lanes:

Western blot - Anti-MAFF antibody - N-terminal (ab227721) at 1/20000 dilution

Lane 1:

Mock transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate

Lane 2:

MAFF transfected HEK-293T whole cell lysate

Predicted band size: 18 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human MAFF. The exact immunogen used to generate this antibody is proprietary information.

Q9ULX9

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/20000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein MAFF also known as v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog F functions as a transcription factor. It is a small protein with a molecular mass of approximately 18 kDa. MAFF is expressed in various tissues including the brain liver and pancreas. It belongs to the small Maf family characterized by their lack of transcriptional activation domains necessitating dimerization with other proteins to regulate gene expression.
Biological function summary

The small Maf protein MAFF plays an important role in regulating oxidative stress response and the coordinated expression of genes involved in detoxification processes. When part of a complex it often dimerizes with Nrf2 a protein that activates the expression of antioxidant response elements (ARE) in gene promoters. This kind of dimerization enables MAFF to indirectly regulate the transcription of genes involved in the cellular protection against oxidative damage.

Pathways

MAFF integrates into the antioxidant defense mechanism especially through its interaction with the Nrf2-Keap1 signaling pathway. Within this pathway MAFF through its interaction with Nrf2 facilitates the induction of cytoprotective genes that help in detoxification and maintaining cellular redox homeostasis. The interaction between MAFF and Nrf2 is significant as Nrf2 becomes stabilized and translocates to the nucleus under oxidative stress which is key for the pathway's protective effects.

MAFF through its interaction mainly with Nrf2 is associated with conditions like cancer and neurodegenerative diseases. In cancer the deregulation of the Nrf2 pathway often involving MAFF can lead to resistance against chemotherapy due to enhanced cellular antioxidant defenses therefore promoting tumor survival. Additionally in neurodegenerative disorders the inefficient activation of the MAFF-Nrf2 pathway may contribute to the accumulation of oxidative damage an underlying factor in diseases like Alzheimer's.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Since they lack a putative transactivation domain, the small Mafs behave as transcriptional repressors when they dimerize among themselves (PubMed : 8932385). However, they seem to serve as transcriptional activators by dimerizing with other (usually larger) basic-zipper proteins, such as NFE2L1/NRF1, and recruiting them to specific DNA-binding sites. Interacts with the upstream promoter region of the oxytocin receptor gene (PubMed : 16549056, PubMed : 8932385). May be a transcriptional enhancer in the up-regulation of the oxytocin receptor gene at parturition (PubMed : 10527846).
See full target information MAFF
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:7228 PubMed40764291

2025

Targeting Msx2 as a brake in the fusion fate of osteoclasts and an anabolic therapy in pre-clinical models of osteoporosis.

Applications

Unspecified application

Species

Unspecified reactive species

Qingliang Ma,Shiyu Wang,Hong Xue,Linhui Ni,Putao Yuan,Yang Shen,Bingjie Zheng,Qingqing Wang,Jiateng Zhang,Haoming Wang,Hongwei Xie,Chao Jiang,An Qin,Shunwu Fan,Ziang Xie,Zhiwei Jie

Renal failure 46:2415517 PubMed39412062

2024

HDAC6 promotes inflammation in lupus nephritis mice by regulating transcription factors MAFF and KLF5 in renal fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Meihui Deng,Xiao Tan,Xiaojie Peng,Weimin Zheng,Rui Fu,Shanshan Tao

Bioengineered 13:13352-13365 PubMed35659199

2022

Yin Yang 1-induced activation of LINC01133 facilitates the progression of pancreatic cancer by sponging miR-199b-5p to upregulate myelin regulatory factor expression.

Applications

Unspecified application

Species

Unspecified reactive species

Xi Yang,Leiming Wang,Fei Zhou,Song Ye,Qianghu Sun
View all publications
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