AB154318

Anti-MAFG antibody

Name: Anti-MAFG antibody (ab154318) | Abcam
Brand: Abcam Limited
SKU: AB154318

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(12 Publications)

Rabbit Polyclonal MAFG antibody. Suitable for IP, IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 12 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MAFG aa 1 to C-terminus.

View Alternative Names

Transcription factor MafG, V-maf musculoaponeurotic fibrosarcoma oncogene homolog G, hMAF, MAFG

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAFG antibody (AB154318)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAFG antibody (AB154318)

Immunohistochemical analysis of paraffin-embedded Human PC9 xenograft tissue labeling MAFG with ab154318 at 1/500 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-MAFG antibody (AB154318)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MAFG antibody (AB154318)

Immunofluorescence analysis of HeLa cells fixed in 4% paraformaldehyde at RT for 15 min labelling MAFG protein at nucleus using ab154318 at a 1/500 dilution (Green). phalloidin, a cytoskeleton marker was stained by anti-phalloidin antibody at a 1/50 dilution (Red).

Scale bar = 10 µm

Supplier Data

Immunoprecipitation - Anti-MAFG antibody (AB154318)

Immunoprecipitation of MAFG protein from HeLa whole cell extracts using 5 µg of MAFG antibody. Western blot analysis was performed using MAFG antibody diluted at 1/500 dilution.

All lanes:

Immunoprecipitation - Anti-MAFG antibody (ab154318) at 1/500 dilution

Lane 1:

HeLa whole cell extracts with control IgG

Lane 2:

HeLa whole cell extracts

Secondary

All lanes:

Anti-Mouse IgG

Predicted band size: 18 kDa

false

Supplier Data

Western blot - Anti-MAFG antibody (AB154318)

Samples were separated by 15 % SDS.

All lanes:

Western blot - Anti-MAFG antibody (ab154318) at 1/1000 dilution

Lane 1:

Mouse muscle tissue extracts at 50 µg

Lane 2:

Rat muscle tissue extracts at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 50 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IHC-P, ICC/IF, WB, IP

applications

Immunogen

Recombinant Fragment Protein within Human MAFG aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O15525

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100 - 1/500", "IP-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MAFG also known as MAFG transcription factor is a small basic leucine zipper (bZIP) protein that weighs approximately 18 kDa. It belongs to the small MAF protein family and has a role in gene regulation. MAFG is widely expressed in various tissues but shows higher expression in the liver and lungs. Functionally MAFG acts as a transcriptional repressor by binding to Maf recognition elements (MAREs) in the DNA regulating genes involved in cellular stress responses and homeostasis.

Biological function summary

The MAFG transcription factor forms complexes with other small MAF proteins like MAFF and MAFK or larger bZIP proteins. These complexes modulate transcription of antioxidant response genes such as those encoding phase II detoxifying enzymes. MAFG is important for the regulation of oxidative stress genes. The interaction of MAFG with other proteins determines whether it functions as a repressor or an activator influencing cellular responses to various stimuli.

Pathways

MAFG plays an important role in the oxidative stress response and the Nrf2 signaling pathway. It interacts closely with Nrf2 an important transcription factor activating the expression of antioxidant proteins to protect against oxidative damage. MAFG and Nrf2 along with other proteins facilitate cellular defense mechanisms by maintaining redox balance and regulating detoxification processes.

MAFG links to conditions such as cancer and neurodegenerative diseases. Aberrant expression of MAFG may contribute to tumor development and progression by disrupting cellular homeostasis and detoxification pathways. Additionally MAFG dysregulation can affect the Nrf2 pathway impacting neurodegenerative disorders like Alzheimer's disease. Therefore understanding MAFG's interaction with Nrf2 and other proteins can provide insights into therapeutic strategies targeting these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Since they lack a putative transactivation domain, the small Mafs behave as transcriptional repressors when they dimerize among themselves (PubMed : 11154691). However, they seem to serve as transcriptional activators by dimerizing with other (usually larger) basic-zipper proteins, such as NFE2, NFE2L1 and NFE2L2, and recruiting them to specific DNA-binding sites (PubMed : 11154691, PubMed : 8932385, PubMed : 9421508). Small Maf proteins heterodimerize with Fos and may act as competitive repressors of the NFE2L2 transcription factor (PubMed : 11154691). Transcription factor, component of erythroid-specific transcription factor NFE2L2 (PubMed : 11154691). Activates globin gene expression when associated with NFE2L2 (PubMed : 11154691). May be involved in signal transduction of extracellular H(+) (By similarity).

See full target information MAFG

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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

Science signaling 16:eadi9018 PubMed38085818

2023

Proximity proteomic analysis of the NRF family reveals the Parkinson's disease protein ZNF746/PARIS as a co-complexed repressor of NRF2.

Applications

Unspecified application

Species

Unspecified reactive species

Kyle M LaPak,Soma Saeidi,Ilah Bok,Nathan T Wamsley,Isaac B Plutzer,Dhaval P Bhatt,Jingqin Luo,Ghazaleh Ashrafi,M Ben Major

Pathogens (Basel, Switzerland) 12: PubMed37375521

2023

Upregulation of Neuropilin-1 Inhibits HTLV-1 Infection.

Applications

Unspecified application

Species

Unspecified reactive species

Wesley Kendle,Kimson Hoang,Erica Korleski,Amanda R Panfil,Nicholas Polakowski,Isabelle Lemasson

PLoS pathogens 19:e1011202 PubMed36827461

2023

HBZ upregulates myoferlin expression to facilitate HTLV-1 infection.

Applications

Unspecified application

Species

Unspecified reactive species

Nicholas Polakowski,Md Abu Kawsar Sarker,Kimson Hoang,Georgina Boateng,Amanda W Rushing,Wesley Kendle,Claudine Pique,Patrick L Green,Amanda R Panfil,Isabelle Lemasson

Frontiers in genetics 13:1068837 PubMed36685838

2023

A novel transcription factor-based signature to predict prognosis and therapeutic response of hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yanbing Yang,Xuenian Ye,Haibin Zhang,Zhaowang Lin,Min Fang,Jian Wang,Yuyan Yu,Xuwen Hua,Hongxuan Huang,Weifeng Xu,Ling Liu,Zhan Lin

JID innovations : skin science from molecules to population health 2:100076 PubMed35146482

2022

A Series of BRAF- and NRAS-Driven Murine Melanoma Cell Lines with Inducible Gene Modulation Capabilities.

Applications

Unspecified application

Species

Unspecified reactive species

Ilah Bok,Ariana Angarita,Stephen M Douglass,Ashani T Weeraratna,Florian A Karreth

Redox biology 50:102239 PubMed35092867

2022

Nrf2 expands the intracellular pool of the chaperone AHSP in a cellular model of β-thalassemia.

Applications

Unspecified application

Species

Unspecified reactive species

Gaijing Han,Cong Cao,Xi Yang,Guo-Wei Zhao,Xin-Jun Hu,Dong-Lin Yu,Rui-Feng Yang,Ke Yang,Ying-Ying Zhang,Wen-Tian Wang,Xiu-Zhen Liu,Peng Xu,Xue-Hui Liu,Ping Chen,Zheng Xue,De-Pei Liu,Xiang Lv

Journal of Cancer 13:146-152 PubMed34976178

2022

LncRNA EIF3J-AS1 functions as an oncogene by regulating MAFG to promote prostate cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

Chen Ye,Shengfei Qin,Fei Guo,Yue Yang,Huiqing Wang,Chao Zhang,Bo Yang

BMC cardiovascular disorders 21:448 PubMed34535081

2021

Proteome-scale profiling reveals MAFF and MAFG as two novel key transcription factors involved in palmitic acid-induced umbilical vein endothelial cell apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Mangyuan Wang,Fen Liu,Binbin Fang,Qiang Huo,Yining Yang

Cancer biology & therapy 22:149-163 PubMed33618612

2021

Silencing of LINC00284 inhibits cell proliferation and migration in oral squamous cell carcinoma by the miR-211-3p/MAFG axis and FUS/KAZN axis.

Applications

Unspecified application

Species

Unspecified reactive species

Dayong Yan,Fuhua Wu,Caixia Peng,Mei Wang

Nature 578:593-599 PubMed32051591

2020

MAFG-driven astrocytes promote CNS inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Michael A Wheeler,Iain C Clark,Emily C Tjon,Zhaorong Li,Stephanie E J Zandee,Charles P Couturier,Brianna R Watson,Giulia Scalisi,Sarah Alkwai,Veit Rothhammer,Assaf Rotem,John A Heyman,Shravan Thaploo,Liliana M Sanmarco,Jiannis Ragoussis,David A Weitz,Kevin Petrecca,Jeffrey R Moffitt,Burkhard Becher,Jack P Antel,Alexandre Prat,Francisco J Quintana

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-MAFG antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAFG antibody (AB154318)

Immunocytochemistry/ Immunofluorescence - Anti-MAFG antibody (AB154318)

Immunoprecipitation - Anti-MAFG antibody (AB154318)

Western blot - Anti-MAFG antibody (AB154318)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (12)

Product promise