AB168453

Anti-MAFG antibody

Name: Anti-MAFG antibody (ab168453) | Abcam
Brand: Abcam Limited
SKU: AB168453
Price: 535 USD
Availability: InStock

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(1 Publication)

Mouse Polyclonal MAFG antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human MAFG.

View Alternative Names

Transcription factor MafG, V-maf musculoaponeurotic fibrosarcoma oncogene homolog G, hMAF, MAFG

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-MAFG antibody (AB168453)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-MAFG antibody (AB168453)

Immunocytochemistry/Immunofluorescence of Daoy cells labeling MAFG with ab168453 at 10μg/ml.

Unknown

Western blot - Anti-MAFG antibody (AB168453)

All lanes:

Western blot - Anti-MAFG antibody (ab168453) at 1 µg/mL

Lane 1:

MAGF transfected 293T cell lysate at 15 µL

Lane 2:

Non-transfected 293T cell lysate at 15 µL

Secondary

All lanes:

Goat Anti-mouse HRP at 1/2500 dilution

Predicted band size: 18 kDa

false

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Full Length Protein corresponding to Human MAFG.

O15525

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MAFG also known as MAFG transcription factor is a small basic leucine zipper (bZIP) protein that weighs approximately 18 kDa. It belongs to the small MAF protein family and has a role in gene regulation. MAFG is widely expressed in various tissues but shows higher expression in the liver and lungs. Functionally MAFG acts as a transcriptional repressor by binding to Maf recognition elements (MAREs) in the DNA regulating genes involved in cellular stress responses and homeostasis.

Biological function summary

The MAFG transcription factor forms complexes with other small MAF proteins like MAFF and MAFK or larger bZIP proteins. These complexes modulate transcription of antioxidant response genes such as those encoding phase II detoxifying enzymes. MAFG is important for the regulation of oxidative stress genes. The interaction of MAFG with other proteins determines whether it functions as a repressor or an activator influencing cellular responses to various stimuli.

Pathways

MAFG plays an important role in the oxidative stress response and the Nrf2 signaling pathway. It interacts closely with Nrf2 an important transcription factor activating the expression of antioxidant proteins to protect against oxidative damage. MAFG and Nrf2 along with other proteins facilitate cellular defense mechanisms by maintaining redox balance and regulating detoxification processes.

MAFG links to conditions such as cancer and neurodegenerative diseases. Aberrant expression of MAFG may contribute to tumor development and progression by disrupting cellular homeostasis and detoxification pathways. Additionally MAFG dysregulation can affect the Nrf2 pathway impacting neurodegenerative disorders like Alzheimer's disease. Therefore understanding MAFG's interaction with Nrf2 and other proteins can provide insights into therapeutic strategies targeting these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Since they lack a putative transactivation domain, the small Mafs behave as transcriptional repressors when they dimerize among themselves (PubMed : 11154691). However, they seem to serve as transcriptional activators by dimerizing with other (usually larger) basic-zipper proteins, such as NFE2, NFE2L1 and NFE2L2, and recruiting them to specific DNA-binding sites (PubMed : 11154691, PubMed : 8932385, PubMed : 9421508). Small Maf proteins heterodimerize with Fos and may act as competitive repressors of the NFE2L2 transcription factor (PubMed : 11154691). Transcription factor, component of erythroid-specific transcription factor NFE2L2 (PubMed : 11154691). Activates globin gene expression when associated with NFE2L2 (PubMed : 11154691). May be involved in signal transduction of extracellular H(+) (By similarity).

See full target information MAFG

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular immunology 119:132-143 PubMed32014632

2020

Camelid type I interferons: Identification and functional characterization of interferon alpha from the dromedary camel (Camelus dromedarius).

Applications

Unspecified application

Species

Unspecified reactive species

Avinash Premraj,Abi George Aleyas,Binita Nautiyal,Thaha Jamal Rasool

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MAFG antibody

Immunocytochemistry/ Immunofluorescence - Anti-MAFG antibody (AB168453)

Western blot - Anti-MAFG antibody (AB168453)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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