AB229766

Anti-MAFK antibody

Name: Anti-MAFK antibody (ab229766) | Abcam
Brand: Abcam Limited
SKU: AB229766

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(2 Publications)

Rabbit Polyclonal MAFK antibody. Suitable for IP, ChIP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human MAFK.

View Alternative Names

Transcription factor MafK, Erythroid transcription factor NF-E2 p18 subunit, MAFK

3 Images

Supplier Data

Immunoprecipitation - Anti-MAFK antibody (AB229766)

MAFK was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate with 4 μg of ab229766. Western blot was performed from the immunoprecipitate using ab229766 at 1/500 dilution.

Lane 1 : Control IgG instead of ab229766 in Jurkat whole cel lysate (input).
Lane 2 : ab229766 IP in Jurkat whole cell lysate.

All lanes:

Immunoprecipitation - Anti-MAFK antibody (ab229766)

Predicted band size: 18 kDa

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ChIP

Supplier Data

ChIP - Anti-MAFK antibody (AB229766)

Cross-linked ChIP was performed with HepG2 (human liver hepatocellular carcinoma cell line) chromatin extract treated with LPS (10 ng/ml for 1 h) using 5 μg of either normal rabbit IgG or ab229766.
The precipitated DNA was detected by PCR with primer set targeting to IL-8 or GAPDH.

Supplier Data

Western blot - Anti-MAFK antibody (AB229766)

15% SDS-PAGE gel.

All lanes:

Western blot - Anti-MAFK antibody (ab229766) at 1/500 dilution

All lanes:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate

Predicted band size: 18 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ChIP, WB, IP

applications

Immunogen

Recombinant Full Length Protein corresponding to Human MAFK.

O60675

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100 - 1/500", "IP-species-notes": "", "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chicken": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MAFK also known as Small Maf protein functions as a transcription factor involved in gene regulation. It has a molecular weight of approximately 18 kDa. This protein mainly acts as a repressor that binds to Maf recognition elements (MAREs) in DNA. Expression of MAFK occurs in various tissues including liver brain and lung. Its ability to form dimers further influences its regulatory activity enabling it to participate in diverse cellular processes.

Biological function summary

MAFK regulates transcription by forming heterodimers with other proteins such as the CNC-bZIP family members including NFE2L2 (also called NRF2). In these complexes MAFK modulates antioxidant response elements (ARE) gene expression. By controlling genes involved in oxidative stress responses MAFK plays an important role in maintaining cellular homeostasis. This functionality allows MAFK to impact a broad range of cellular activities reinforcing its importance in cellular responses to stress.

Pathways

MAFK is heavily involved in the oxidative stress response and the xenobiotic metabolism pathway. Through its interaction with NRF2 MAFK influences the cellular defense mechanism against oxidative damage highlighting its role in the NRF2-ARE pathway. Moreover MAFK also intersects with the heme degradation pathway where it collaborates with heme oxygenase 1 (HO-1) regulation. By participating in these pathways MAFK supports cellular adaptation to environmental changes.

MAFK has connections to cancer and neurodegenerative diseases. In various cancers altered MAFK expression impacts tumor growth and progression partly through NRF2-related pathways. Abnormal regulation of MAFK can also relate to disorders like Parkinson's disease where oxidative stress mechanisms are dysregulated. In these contexts the protein interacts with others like KEAP1 a regulator of NRF2 activity to influence disease development and progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Since they lack a putative transactivation domain, the small Mafs behave as transcriptional repressors when they dimerize among themselves (PubMed : 9150357). However, they act as transcriptional activators by dimerizing with other (usually larger) basic-zipper proteins, such as NFE2, NFE2L1/NRF1, NFE2L2/NRF2 and NFE2L3/NRF3, and recruiting them to specific DNA-binding sites (PubMed : 8932385, PubMed : 9150357). Small Maf proteins heterodimerize with Fos and may act as competitive repressors of the NF-E2 transcription factor (PubMed : 9150357).

See full target information MAFK

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Redox biology 50:102239 PubMed35092867

2022

Nrf2 expands the intracellular pool of the chaperone AHSP in a cellular model of β-thalassemia.

Applications

Unspecified application

Species

Unspecified reactive species

Gaijing Han,Cong Cao,Xi Yang,Guo-Wei Zhao,Xin-Jun Hu,Dong-Lin Yu,Rui-Feng Yang,Ke Yang,Ying-Ying Zhang,Wen-Tian Wang,Xiu-Zhen Liu,Peng Xu,Xue-Hui Liu,Ping Chen,Zheng Xue,De-Pei Liu,Xiang Lv

International journal of molecular medicine 45:23-32 PubMed31939616

2020

Loss of LAMTOR1 in pancreatic β‑cells increases glucose‑stimulated insulin secretion in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Qiong Huang,Qi Gong,Ting Wen,Shanshan Feng,Jixiong Xu,Jianying Liu,Xiudan Han,Qun Liu,Juan Hu,Lingyan Zhu

View all publications

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-MAFK antibody

Immunoprecipitation - Anti-MAFK antibody (AB229766)

ChIP - Anti-MAFK antibody (AB229766)

Western blot - Anti-MAFK antibody (AB229766)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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