AB50322

Anti-MAFK antibody

Name: Anti-MAFK antibody (ab50322) | Abcam
Brand: Abcam Limited
SKU: AB50322
Price: 545 USD
Availability: InStock
Rating: 4 (2 reviews)

(2 Reviews)

(9 Publications)

Rabbit Polyclonal MAFK antibody. Suitable for WB and reacts with Human samples. Cited in 9 publications. Immunogen corresponding to Synthetic Peptide within Human MAFK aa 1-50 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

Transcription factor MafK, Erythroid transcription factor NF-E2 p18 subunit, MAFK

1 Images

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Western blot - Anti-MAFK antibody (AB50322)

Lanes 1 and 3:

Western blot - Anti-MAFK antibody (ab50322) at 1/4000 dilution

Lane 2:

Western blot - Anti-MAFK antibody (ab50322) at 1/8000 dilution

Lanes 1 - 2:

A431 whole cell lysate

Lane 3:

A431 whole cell lysate with MAFF immunizing peptide

Lane 4:

A431 whole cell lysate with A431 whole cell lysate

Secondary

All lanes:

Goat Anti-Rabbit IgG, Peroxidase conjugate

Predicted band size: 18 kDa

Observed band size: 18 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Synthetic Peptide within Human MAFK aa 1-50 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

O60675

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/4000 - 1/8000", "WB-species-notes": "<p>Dilute the antibody in PBS containing 5.0% non-fat dry milk and 0.05 % Tween 20. Staining of the MAFF band in immunoblotting is specifically inhibited by the immunizing peptide.</p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Ion exchange chromatography

Storage buffer

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MAFK also known as Small Maf protein functions as a transcription factor involved in gene regulation. It has a molecular weight of approximately 18 kDa. This protein mainly acts as a repressor that binds to Maf recognition elements (MAREs) in DNA. Expression of MAFK occurs in various tissues including liver brain and lung. Its ability to form dimers further influences its regulatory activity enabling it to participate in diverse cellular processes.

Biological function summary

MAFK regulates transcription by forming heterodimers with other proteins such as the CNC-bZIP family members including NFE2L2 (also called NRF2). In these complexes MAFK modulates antioxidant response elements (ARE) gene expression. By controlling genes involved in oxidative stress responses MAFK plays an important role in maintaining cellular homeostasis. This functionality allows MAFK to impact a broad range of cellular activities reinforcing its importance in cellular responses to stress.

Pathways

MAFK is heavily involved in the oxidative stress response and the xenobiotic metabolism pathway. Through its interaction with NRF2 MAFK influences the cellular defense mechanism against oxidative damage highlighting its role in the NRF2-ARE pathway. Moreover MAFK also intersects with the heme degradation pathway where it collaborates with heme oxygenase 1 (HO-1) regulation. By participating in these pathways MAFK supports cellular adaptation to environmental changes.

MAFK has connections to cancer and neurodegenerative diseases. In various cancers altered MAFK expression impacts tumor growth and progression partly through NRF2-related pathways. Abnormal regulation of MAFK can also relate to disorders like Parkinson's disease where oxidative stress mechanisms are dysregulated. In these contexts the protein interacts with others like KEAP1 a regulator of NRF2 activity to influence disease development and progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Since they lack a putative transactivation domain, the small Mafs behave as transcriptional repressors when they dimerize among themselves (PubMed : 9150357). However, they act as transcriptional activators by dimerizing with other (usually larger) basic-zipper proteins, such as NFE2, NFE2L1/NRF1, NFE2L2/NRF2 and NFE2L3/NRF3, and recruiting them to specific DNA-binding sites (PubMed : 8932385, PubMed : 9150357). Small Maf proteins heterodimerize with Fos and may act as competitive repressors of the NF-E2 transcription factor (PubMed : 9150357).

See full target information MAFK

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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Science signaling 16:eadi9018 PubMed38085818

2023

Proximity proteomic analysis of the NRF family reveals the Parkinson's disease protein ZNF746/PARIS as a co-complexed repressor of NRF2.

Applications

Unspecified application

Species

Unspecified reactive species

Kyle M LaPak,Soma Saeidi,Ilah Bok,Nathan T Wamsley,Isaac B Plutzer,Dhaval P Bhatt,Jingqin Luo,Ghazaleh Ashrafi,M Ben Major

Cell death & disease 12:349 PubMed33824282

2021

ZEB1-induced LINC01559 expedites cell proliferation, migration and EMT process in gastric cancer through recruiting IGF2BP2 to stabilize ZEB1 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Huojian Shen,Hongyi Zhu,Yuanwen Chen,Zhiyong Shen,Weiqing Qiu,Changlin Qian,Jie Zhang

Molecular nutrition & food research 61: PubMed27860235

2017

A functional pseudogene, NMRAL2P, is regulated by Nrf2 and serves as a coactivator of NQO1 in sulforaphane-treated colon cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Gavin S Johnson,Jia Li,Laura M Beaver,W Mohaiza Dashwood,Deqiang Sun,Praveen Rajendran,David E Williams,Emily Ho,Roderick H Dashwood

Journal of immunology (Baltimore, Md. : 1950) 194:1928-37 PubMed25567680

2015

TAM receptor-dependent regulation of SOCS3 and MAPKs contributes to proinflammatory cytokine downregulation following chronic NOD2 stimulation of human macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Shasha Zheng,Matija Hedl,Clara Abraham

American journal of respiratory cell and molecular biology 52:387-96 PubMed25259561

2014

Oxidative stress regulates CFTR gene expression in human airway epithelial cells through a distal antioxidant response element.

Applications

Unspecified application

Species

Unspecified reactive species

Zhaolin Zhang,Shih-Hsing Leir,Ann Harris

BMB reports 47:524-9 PubMed25059280

2014

Oleanolic acid regulates NF-κB signaling by suppressing MafK expression in RAW 264.7 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Jin Hwang,Jaewhan Song,Haeng-Ran Kim,Kyung-A Hwang

PloS one 9:e84713 PubMed24465428

2014

Evidence for site-specific occupancy of the mitochondrial genome by nuclear transcription factors.

Applications

ICC/IF

Species

Human

Georgi K Marinov,Yun E Wang,David Chan,Barbara J Wold

Cell 152:327-39 PubMed23332764

2013

DNA-binding specificities of human transcription factors.

Applications

Unspecified application

Species

Unspecified reactive species

Arttu Jolma,Jian Yan,Thomas Whitington,Jarkko Toivonen,Kazuhiro R Nitta,Pasi Rastas,Ekaterina Morgunova,Martin Enge,Mikko Taipale,Gonghong Wei,Kimmo Palin,Juan M Vaquerizas,Renaud Vincentelli,Nicholas M Luscombe,Timothy R Hughes,Patrick Lemaire,Esko Ukkonen,Teemu Kivioja,Jussi Taipale

Journal of proteomics 80:132-44 PubMed23291531

2013

Identification of KAP-1-associated complexes negatively regulating the Ey and β-major globin genes in the β-globin locus.

Applications

ChIP

Species

Unspecified reactive species

Dong Yang,Zhanfu Ma,Weiran Lin,Juntao Yang,Chunyan Tian,Handong Wei,Ying Jiang,Fuchu He

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-MAFK antibody

Western blot - Anti-MAFK antibody (AB50322)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (9)

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