Mouse Monoclonal MAG/GMA antibody. Carrier free. Suitable for ICC, Flow Cyt, WB and reacts with Human, Recombinant fragment - Human samples. Cited in 62 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MAG aa 100-250.
Images
![Flow Cytometry - Anti-MAG/GMA antibody [3C7] - BSA and Azide free (AB89780), expandable thumbnail](https://content.abcam.com/products/images/mag-gma-antibody-3c7-bsa-and-azide-free-ab89780--flow-cytometry-img39808.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-MAG/GMA antibody [3C7] - BSA and Azide free (AB89780)")
![Western blot - Anti-MAG/GMA antibody [3C7] - BSA and Azide free (AB89780), expandable thumbnail](https://content.abcam.com/products/images/mag-gma-antibody-3c7-bsa-and-azide-free-ab89780--western-blot-img160440.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-MAG/GMA antibody [3C7] - BSA and Azide free (AB89780)")
![Immunocytochemistry - Anti-MAG/GMA antibody [3C7] - BSA and Azide free (AB89780), expandable thumbnail](https://content.abcam.com/products/images/mag-gma-antibody-3c7-bsa-and-azide-free-ab89780--immunocytochemistry-img73119.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry - Anti-MAG/GMA antibody [3C7] - BSA and Azide free (AB89780)")
![Western blot - Anti-MAG/GMA antibody [3C7] - BSA and Azide free (AB89780), expandable thumbnail](https://content.abcam.com/products/images/mag-gma-antibody-3c7-bsa-and-azide-free-ab89780--western-blot-img35128.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-MAG/GMA antibody [3C7] - BSA and Azide free (AB89780)")
Publications
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- Genome medicine 15:1092023Acute ischemia induces spatially and transcriptionally distinct microglial subclusters.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHuiya Li,Pinyi Liu,Bing Zhang,Zengqiang Yuan,Mengdi Guo,Xinxin Zou,Yi Qian,Shiji Deng,Liwen Zhu,Xiang Cao,Tao Tao,Shengnan Xia,Xinyu Bao,Yun XuPubMed 38082331
- Nature communications 14:70902023Systemic and intrinsic functions of ATRX in glial cell fate and CNS myelination in male mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMegan E Rowland,Yan Jiang,Sarfraz Shafiq,Alireza Ghahramani,Miguel A Pena-Ortiz,Vanessa Dumeaux,Nathalie G BérubéPubMed 37925436
- JCI insight 8:2023MOGAD patient autoantibodies induce complement, phagocytosis, and cellular cytotoxicity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSoumya S Yandamuri,Beata Filipek,Abeer H Obaid,Nikhil Lele,Joshua M Thurman,Naila Makhani,Richard J Nowak,Yong Guo,Claudia F Lucchinetti,Eoin P Flanagan,Erin E Longbrake,Kevin C O'ConnorPubMed 37097758
- Cell & bioscience 13:422023ANGPTL2 binds MAG to efficiently enhance oligodendrocyte differentiation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLu Chen,Zhuo Yu,Li Xie,Xiaoxiao He,Xingmei Mu,Chiqi Chen,Wenqian Yang,Xiaoping Tong,Junling Liu,Zhengliang Gao,Suya Sun,NanJie Xu,Zhigang Lu,Junke Zheng,Yaping ZhangPubMed 36855057
- Advanced science (Weinheim, Baden-Wurttemberg, Germany) 10:e22029762022Optogenetic Stimulation of mPFC Alleviates White Matter Injury-Related Cognitive Decline after Chronic Ischemia through Adaptive Myelination.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShiji Deng,Shu Shu,Lili Zhai,Shengnan Xia,Xiang Cao,Huiya Li,Xinyu Bao,Pinyi Liu,Yun XuPubMed 36529961
- International journal of molecular medicine 51:2022Activin A rescues preterm brain injury through a novel Noggin/BMP4/Id2 signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaojuan Su,Junjie Ying,Dongqiong Xiao,Xia Qiu,Shiping Li,Fengyan Zhao,Jun TangPubMed 36524372
- Nature communications 13:68132022Vascular endothelium deploys caveolin-1 to regulate oligodendrogenesis after chronic cerebral ischemia in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYing Zhao,Wusheng Zhu,Ting Wan,Xiaohao Zhang,Yunzi Li,Zhenqian Huang,Pengfei Xu,Kangmo Huang,Ruidong Ye,Yi Xie,Xinfeng LiuPubMed 36357389
- Glia 71:541-5592022Deletion of Nox4 enhances remyelination following cuprizone-induced demyelination by increasing phagocytic capacity of microglia and macrophages in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKei Yamanaka,Kuniyuki Nakamura,Tomoya Shibahara,Masamitsu Takashima,Hayato Takaki,Masaoki Hidaka,Motohiro Komori,Yoji Yoshikawa,Yoshinobu Wakisaka,Tetsuro Ago,Takanari KitazonoPubMed 36321558
- Cell death & disease 13:5852022Cannabinoid CB receptor gene inactivation in oligodendrocyte precursors disrupts oligodendrogenesis and myelination in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAníbal Sánchez-de la Torre,Tania Aguado,Alba Huerga-Gómez,Silvia Santamaría,Antonietta Gentile,Juan Carlos Chara,Carlos Matute,Krisztina Monory,Susana Mato,Manuel Guzmán,Beat Lutz,Ismael Galve-Roperh,Javier PalazuelosPubMed 35798697
- Nature communications 13:11342022Astrocytic phagocytosis contributes to demyelination after focal cortical ischemia in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTing Wan,Wusheng Zhu,Ying Zhao,Xiaohao Zhang,Ruidong Ye,Meng Zuo,Pengfei Xu,Zhenqian Huang,Chunni Zhang,Yi Xie,Xinfeng LiuPubMed 35241660
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Fragment Protein within Human MAG aa 100-250. The exact immunogen used to generate this antibody is proprietary information. Database link P20916
Reactivity data
Select an application| ICC | Flow Cyt | WB | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Orangutan | Predicted | Predicted | Predicted |
| Recombinant fragment - Human | Not recommended | Not recommended | Tested |
ICC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Orangutan | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Orangutan | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes Isoform a, 69 kDa; Isoform b, 63 kDa. |
Species Human | Dilution info - | Notes Isoform a, 69 kDa; Isoform b, 63 kDa. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Orangutan | Dilution info - | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
Adhesion molecule that mediates interactions between myelinating cells and neurons by binding to neuronal sialic acid-containing gangliosides and to the glycoproteins RTN4R and RTN4RL2 (By similarity). Not required for initial myelination, but seems to play a role in the maintenance of normal axon myelination. Protects motoneurons against apoptosis, also after injury; protection against apoptosis is probably mediated via interaction with neuronal RTN4R and RTN4RL2. Required to prevent degeneration of myelinated axons in adults; this probably depends on binding to gangliosides on the axon cell membrane (By similarity). Negative regulator of neurite outgrowth; in dorsal root ganglion neurons the inhibition is mediated primarily via binding to neuronal RTN4R or RTN4RL2 and to a lesser degree via binding to neuronal gangliosides. In cerebellar granule cells the inhibition is mediated primarily via binding to neuronal gangliosides. In sensory neurons, inhibition of neurite extension depends only partially on RTN4R, RTN4RL2 and gangliosides. Inhibits axon longitudinal growth (By similarity). Inhibits axon outgrowth by binding to RTN4R (By similarity). Preferentially binds to alpha-2,3-linked sialic acid. Binds ganglioside Gt1b (By similarity).
Alternative names
GMA, MAG, Myelin-associated glycoprotein, Siglec-4a
Recommended products
Mouse Monoclonal MAG/GMA antibody. Carrier free. Suitable for ICC, Flow Cyt, WB and reacts with Human, Recombinant fragment - Human samples. Cited in 62 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MAG aa 100-250.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Fragment Protein within Human MAG aa 100-250. The exact immunogen used to generate this antibody is proprietary information. Database link P20916
- Carrier free
- Yes
- Clone number
- 3C7
- Purification technique
- Affinity purification Protein A
- Light chain type
- kappa
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product was changed from ascites to tissue culture supernatant on 4th April 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MAG (Myelin-Associated Glycoprotein) sometimes referred to as 'MAG protein' 'MAG RAT' or 'MAG/GMA' weighs approximately 100 kDa. This protein is prominently expressed in the central and peripheral nervous systems. MAG localizes to the periaxonal region of myelinated axons making it essential for proper neuronal function. It plays an important role in maintaining the stability and structure of myelin sheaths which are important for the efficient transmission of nerve impulses.
- Biological function summary
This protein acts as an adhesion molecule that contributes to the myelination process. It exists as part of a molecular complex that interacts with other oligodendrocyte and Schwann cell proteins. This interaction is important for nerve regeneration and the expansion of the myelin sheath during development and repair. MAG also assists in signaling between axons and glial cells improving cellular communication and myelin integrity.
- Pathways
MAG significantly impacts pathways such as the Nogo receptor which is associated with inhibiting axonal growth and regeneration. MAG interacts with the NOGO protein and other members of the Nogo receptor signaling pathway where it functions to regulate neuronal growth and regeneration. Furthermore MAG's interplay with NOGO receptor components contributes to limiting excessive sprouting of axons balancing growth and stability of the nervous system.
- Associated diseases and disorders
MAG is notably linked to neuropathies and multiple sclerosis. In neuropathies abnormal MAG expression can disrupt nerve conduction and lead to demyelinating conditions. Multiple sclerosis sees extensive involvement of MAG where its malfunction or reduced expression associates with the failure of remyelination processes. Additionally MAG's connection with proteins such as NOGO and other myelin-associated inhibitors implicates its role in the pathology of these disorders offering potential targets for therapeutic interventions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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4 product images
![Flow Cytometry - Anti-MAG/GMA antibody [3C7] - BSA and Azide free (ab89780), expandable thumbnail](https://content.abcam.com/products/images/mag-gma-antibody-3c7-bsa-and-azide-free-ab89780--flow-cytometry-img39808.jpg "Flow Cytometry - Anti-MAG/GMA antibody [3C7] - BSA and Azide free (ab89780)")
Flow Cytometry - Anti-MAG/GMA antibody [3C7] - BSA and Azide free (ab89780)
Overlay histogram showing SH-SY5Y cells stained with ab89780 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab89780, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control ab91353, 2μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min) used under the same conditions.
Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.This image was generated using the ascites version of the product.
![Western blot - Anti-MAG/GMA antibody [3C7] - BSA and Azide free (ab89780), expandable thumbnail](https://content.abcam.com/products/images/mag-gma-antibody-3c7-bsa-and-azide-free-ab89780--western-blot-img160440.jpg "Western blot - Anti-MAG/GMA antibody [3C7] - BSA and Azide free (ab89780)")
Western blot - Anti-MAG/GMA antibody [3C7] - BSA and Azide free (ab89780)
This image was generated using the ascites version of the product.
All lanes: Western blot - Anti-MAG/GMA antibody [3C7] - BSA and Azide free (ab89780) at 5 µg/mL
All lanes: Jurkat cell lysate at 50 µg
Predicted band size: 69 kDa
Observed band size: 53 kDa
![Immunocytochemistry - Anti-MAG/GMA antibody [3C7] - BSA and Azide free (ab89780), expandable thumbnail](https://content.abcam.com/products/images/mag-gma-antibody-3c7-bsa-and-azide-free-ab89780--immunocytochemistry-img73119.jpg "Immunocytochemistry - Anti-MAG/GMA antibody [3C7] - BSA and Azide free (ab89780)")
Immunocytochemistry - Anti-MAG/GMA antibody [3C7] - BSA and Azide free (ab89780)
ab89780 stained SKNSH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab89780 at 10μg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.
This image was generated using the ascites version of the product.
![Western blot - Anti-MAG/GMA antibody [3C7] - BSA and Azide free (ab89780), expandable thumbnail](https://content.abcam.com/products/images/mag-gma-antibody-3c7-bsa-and-azide-free-ab89780--western-blot-img35128.jpg "Western blot - Anti-MAG/GMA antibody [3C7] - BSA and Azide free (ab89780)")
Western blot - Anti-MAG/GMA antibody [3C7] - BSA and Azide free (ab89780)
Western blot against tagged recombinant protein immunogen. Predicted band size of immunogen is 36 kDa.
This image was generated using the ascites version of the product.
All lanes: Western blot - Anti-MAG/GMA antibody [3C7] - BSA and Azide free (ab89780) at 5 µg/mL
All lanes: Partial tagged recombinant Human MAG/GMA protein at 0.2 µg
Predicted band size: 69 kDa
Observed band size: 36 kDa
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