Anti-MAG/GMA antibody [3C7] - BSA and Azide free

• ICC

Lab

Immunocytochemistry - Anti-MAG/GMA antibody [3C7] - BSA and Azide free (AB89780)

ab89780 stained SKNSH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab89780 at 10μg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

This image was generated using the ascites version of the product.

• Flow Cyt

Unknown

Flow Cytometry - Anti-MAG/GMA antibody [3C7] - BSA and Azide free (AB89780)

Overlay histogram showing SH-SY5Y cells stained with ab89780 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab89780, 1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight^® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min) used under the same conditions.
Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

This image was generated using the ascites version of the product.

• WB

Unknown

Western blot - Anti-MAG/GMA antibody [3C7] - BSA and Azide free (AB89780)

This image was generated using the ascites version of the product.

All lanes:

Western blot - Anti-MAG/GMA antibody [3C7] - BSA and Azide free (ab89780) at 5 µg/mL

All lanes:

Jurkat cell lysate at 50 µg

Predicted band size: 69 kDa

Observed band size: 53 kDa

false

• WB

Unknown

Western blot - Anti-MAG/GMA antibody [3C7] - BSA and Azide free (AB89780)

Western blot against tagged recombinant protein immunogen. Predicted band size of immunogen is 36 kDa.

This image was generated using the ascites version of the product.

All lanes:

Western blot - Anti-MAG/GMA antibody [3C7] - BSA and Azide free (ab89780) at 5 µg/mL

All lanes:

Partial tagged recombinant Human MAG/GMA protein at 0.2 µg

Predicted band size: 69 kDa

Observed band size: 36 kDa

false