Anti-MAG/GMA antibody [EPR24276-125] Rabbit monoclonal (ab277524)

Rabbit Recombinant Monoclonal MAG/GMA antibody. Suitable for ICC/IF, IP, WB, IHC-Fr, IHC-P and reacts with Rat, Mouse, Human samples. Cited in 2 publications.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAG/GMA antibody [EPR24276-125] (AB277524), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	ICC/IF	IP	Flow Cyt	WB	IHC-Fr	IHC-P
Human	Not recommended	Tested	Not recommended	Tested	Expected	Tested
Mouse	Not recommended	Tested	Not recommended	Tested	Tested	Tested
Rat	Tested	Expected	Not recommended	Tested	Tested	Tested

Tested
Tested

Species	Dilution info	Notes
Species Rat	Dilution info 1/50	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/30	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info 1/30	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Rat, Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/1000	Notes -
Species Rat	Dilution info 1/1000	Notes -
Species Human	Dilution info 1/1000	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/500	Notes Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Species Rat	Dilution info 1/500	Notes Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Expected
Expected

Species	Dilution info	Notes
Species Human	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/2000	Notes Can be used at 1/5000 dilution for human. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/2000	Notes Can be used at 1/5000 dilution for human. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info 1/2000	Notes Can be used at 1/5000 dilution for human. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target data

See full target information MAG

Function

Adhesion molecule that mediates interactions between myelinating cells and neurons by binding to neuronal sialic acid-containing gangliosides and to the glycoproteins RTN4R and RTN4RL2 (By similarity). Not required for initial myelination, but seems to play a role in the maintenance of normal axon myelination. Protects motoneurons against apoptosis, also after injury; protection against apoptosis is probably mediated via interaction with neuronal RTN4R and RTN4RL2. Required to prevent degeneration of myelinated axons in adults; this probably depends on binding to gangliosides on the axon cell membrane (By similarity). Negative regulator of neurite outgrowth; in dorsal root ganglion neurons the inhibition is mediated primarily via binding to neuronal RTN4R or RTN4RL2 and to a lesser degree via binding to neuronal gangliosides. In cerebellar granule cells the inhibition is mediated primarily via binding to neuronal gangliosides. In sensory neurons, inhibition of neurite extension depends only partially on RTN4R, RTN4RL2 and gangliosides. Inhibits axon longitudinal growth (By similarity). Inhibits axon outgrowth by binding to RTN4R (By similarity). Preferentially binds to alpha-2,3-linked sialic acid. Binds ganglioside Gt1b (By similarity).

Alternative names

GMA, MAG, Myelin-associated glycoprotein, Siglec-4a

Recommended products

Rabbit Recombinant Monoclonal MAG/GMA antibody. Suitable for ICC/IF, IP, WB, IHC-Fr, IHC-P and reacts with Rat, Mouse, Human samples. Cited in 2 publications.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR24276-125
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

MAG (Myelin-Associated Glycoprotein) sometimes referred to as 'MAG protein' 'MAG RAT' or 'MAG/GMA' weighs approximately 100 kDa. This protein is prominently expressed in the central and peripheral nervous systems. MAG localizes to the periaxonal region of myelinated axons making it essential for proper neuronal function. It plays an important role in maintaining the stability and structure of myelin sheaths which are important for the efficient transmission of nerve impulses.

Biological function summary

This protein acts as an adhesion molecule that contributes to the myelination process. It exists as part of a molecular complex that interacts with other oligodendrocyte and Schwann cell proteins. This interaction is important for nerve regeneration and the expansion of the myelin sheath during development and repair. MAG also assists in signaling between axons and glial cells improving cellular communication and myelin integrity.

Pathways

MAG significantly impacts pathways such as the Nogo receptor which is associated with inhibiting axonal growth and regeneration. MAG interacts with the NOGO protein and other members of the Nogo receptor signaling pathway where it functions to regulate neuronal growth and regeneration. Furthermore MAG's interplay with NOGO receptor components contributes to limiting excessive sprouting of axons balancing growth and stability of the nervous system.

Associated diseases and disorders

MAG is notably linked to neuropathies and multiple sclerosis. In neuropathies abnormal MAG expression can disrupt nerve conduction and lead to demyelinating conditions. Multiple sclerosis sees extensive involvement of MAG where its malfunction or reduced expression associates with the failure of remyelination processes. Additionally MAG's connection with proteins such as NOGO and other myelin-associated inhibitors implicates its role in the pathology of these disorders offering potential targets for therapeutic interventions.

Product promise

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13 product images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAG/GMA antibody [EPR24276-125] (ab277524)
Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling MAG/GMA with ab277524 at 1/5000 dilution (0.1008 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on human cerebrum.The section was incubated with ab277524 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunoprecipitation - Anti-MAG/GMA antibody [EPR24276-125] (ab277524)
MAG/GMA was immunoprecipitated from 0.35 mg human cerebellum tissue lysate with ab277524 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab277524 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: Human cerebellum tissue lysate 10 ug
Lane 2: ab277524 IP in Human cerebellum tissue lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab277524 in Human cerebellum tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 23 seconds.
All lanes: Immunoprecipitation - Anti-MAG/GMA antibody [EPR24276-125] (ab277524)
Predicted band size: 69 kDa
Observed band size: 100 kDa
Immunohistochemistry (Frozen sections) - Anti-MAG/GMA antibody [EPR24276-125] (ab277524)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse cerebellum tissue labeling MAG/GMA with ab277524 at 1/500 dilution (1.008 ug/ml) followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution (Green). Positive staining on mouse cerebellum is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Immunocytochemistry/ Immunofluorescence - Anti-MAG/GMA antibody [EPR24276-125] (ab277524)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized rat primary neural/glia cell cells labelling MAG/GMA with ab277524 at 1/50 dilution (10.08 ug/ml), followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 594) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in rat primary oligodendroglia cells. Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection. Anti-Myelin Basic Protein rat monoclonal antibody was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
-ve control 1: Anti-Sortilin/NT3 antibody [EPR23093-81] - BSA and Azide free (Capture) ab277241 at 1/50 dilution, followed by Goat Anti-Rat IgG H&L (Alexa Fluor® 488) ab150157 at 1/1000 dilution.
-ve control 2: Anti-Myelin Basic Protein rat monoclonal antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 at 1/1000 dilution.
Western blot - Anti-MAG/GMA antibody [EPR24276-125] (ab277524)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
MAG is a glycoprotein. The molecular weight observed is consistent with what has been described in the literature (PMID: 1716323).
Negative control: Liver (PMID: 2432614).
Exposure time: 26 seconds.
All lanes: Western blot - Anti-MAG/GMA antibody [EPR24276-125] (ab277524) at 1/1000 dilution
Lane 1: Human cerebellum tissue lysate at 20 µg
Lane 2: Human liver tissue lysate at 20 µg
Secondary
All lanes: VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution
Predicted band size: 69 kDa
Observed band size: 100 kDa
Immunoprecipitation - Anti-MAG/GMA antibody [EPR24276-125] (ab277524)
MAG/GMA was immunoprecipitated from 0.35 mg mouse brain tissue lysate with ab277524 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab277524 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: Mouse brain tissue lysate 10 ug
Lane 2: ab277524 IP in Mouse brain tissue lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab277524 in Mouse brain tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 23 seconds.
All lanes: Immunoprecipitation - Anti-MAG/GMA antibody [EPR24276-125] (ab277524)
Predicted band size: 69 kDa
Observed band size: 100 kDa
Immunohistochemistry (Frozen sections) - Anti-MAG/GMA antibody [EPR24276-125] (ab277524)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat cerebellum tissue labeling MAG/GMA with ab277524 at 1/500 dilution (1.008 ug/ml) followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution (Green). Positive staining on rat cerebellum is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Western blot - Anti-MAG/GMA antibody [EPR24276-125] (ab277524)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
MAG is a glycoprotein. The molecular weight observed is consistent with what has been described in the literature (PMID: 1716323).
Negative control: Liver (PMID: 2432614).
Exposure time: 126 seconds.
All lanes: Western blot - Anti-MAG/GMA antibody [EPR24276-125] (ab277524) at 1/1000 dilution
Lane 1: Mouse brain tissue lysate at 20 µg
Lane 2: Mouse cerebellum tissue lysate at 20 µg
Lane 3: Mouse liver tissue lysate at 20 µg
Lane 4: Rat cerebellum tissue lysate at 20 µg
Lane 5: Rat liver tissue lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 69 kDa
Observed band size: 100 kDa
Immunohistochemistry (Frozen sections) - Anti-MAG/GMA antibody [EPR24276-125] (ab277524)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse liver tissue labeling MAG/GMA with ab277524 at 1/100 dilution (5.04 ug/ml) followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution (Green). Negative control: No staining on mouse liver (PMID2432614) is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAG/GMA antibody [EPR24276-125] (ab277524)
Immunohistochemical analysis of paraffin-embedded human sciatic nerve tissue labeling MAG/GMA with ab277524 at 1/5000 dilution (0.1008 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on human sciatic nerve. The section was incubated with ab277524 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Frozen sections) - Anti-MAG/GMA antibody [EPR24276-125] (ab277524)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat liver tissue labeling MAG/GMA with ab277524 at 1/100 dilution (5.04 ug/ml) followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution (Green). Negative control: No staining on rat liver (PMID2432614) is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAG/GMA antibody [EPR24276-125] (ab277524)
Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue labeling MAG/GMA with ab277524 at 1/2000 dilution (0.252 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on mouse hippocampus. (PMID: 25523827)The section was incubated with ab277524 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAG/GMA antibody [EPR24276-125] (ab277524)
Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue labeling MAG/GMA with ab277524 at 1/2000 (0.252 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on rat hippocampus. (PMID: 17705198, 27000654 )The section was incubated with ab277524 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.