Mouse Monoclonal MAGEA4 antibody. Carrier free. Suitable for Protein Array, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human MAGEA4.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAGEA4 antibody [CPTC-MAGEA4-1] - BSA and Azide free (AB268201), expandable thumbnail](https://content.abcam.com/products/images/magea4-antibody-cptc-magea4-1-bsa-and-azide-free-ab268201--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img113159.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAGEA4 antibody [CPTC-MAGEA4-1] - BSA and Azide free (AB268201)")
![Protein Array - Anti-MAGEA4 antibody [CPTC-MAGEA4-1] - BSA and Azide free (AB268201), expandable thumbnail](https://content.abcam.com/products/images/magea4-antibody-cptc-magea4-1-bsa-and-azide-free-ab268201--protein-array-img1219.jpg/_jcr_content/renditions/webp-475.webp "Protein Array - Anti-MAGEA4 antibody [CPTC-MAGEA4-1] - BSA and Azide free (AB268201)")
Key facts
- Isotype
- IgG2c
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human MAGEA4. Database link P43358
Reactivity data
Select an application| Protein Array | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
Protein Array
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1-2 µg/mL | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Associated Products
Select an associated product type
1 product for Alternative Version
2 products for Alternative Product
Target data
Function
Regulates cell proliferation through the inhibition of cell cycle arrest at the G1 phase (PubMed:22842486). Also negatively regulates p53-mediated apoptosis (PubMed:22842486).
Alternative names
MAGE4, MAGEA4, Melanoma-associated antigen 4, Cancer/testis antigen 1.4, MAGE-4 antigen, MAGE-41 antigen, MAGE-X2 antigen, CT1.4
Recommended products
Mouse Monoclonal MAGEA4 antibody. Carrier free. Suitable for Protein Array, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human MAGEA4.
Key facts
- Isotype
- IgG2c
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human MAGEA4. Database link P43358
- Carrier free
- Yes
- Clone number
- CPTC-MAGEA4-1
- Purification technique
- Affinity purification Protein A/G
- Light chain type
- kappa
- Concentration
- Purification notes
Purified from bioreactor concentrate.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
ab268201 is the carrier-free version of Anti-MAGEA4 antibody [CPTC-MAGEA4-1] ab268080.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MAGEA4 also known as MAGE-A4 is part of the melanoma-associated antigen family. This protein is encoded by the MAGEA4 gene and is characterized by a molecular mass of approximately 42 kDa. Its expression is limited to certain tissues including the testis and is often silent in normal somatic tissues. However MAGEA4 is frequently found in various types of tumors making it a target of interest for cancer research and therapy.
- Biological function summary
MAGEA4 participates in cellular processes related to cancer progression. Although not part of a multi-protein complex its presence in tumorous tissues suggests a role in modulating cellular mechanisms that favor tumor survival and proliferation. MAGEA4 does not directly interact with other proteins but might affect cellular pathways indirectly contributing to oncogenesis.
- Pathways
MAGEA4 influences several cellular mechanisms that are related to cancer development. While it is not a central player in major biological pathways its function relates to processes affecting cell cycle regulation and apoptosis. This connection was observed in studies showing interactions with other cancer-related proteins like p53 suggesting that MAGEA4 can modify responses to apoptotic signals and may interact with or affect the TP53 pathway.
- Associated diseases and disorders
MAGEA4 is prominently linked to various cancers including melanomas and carcinomas such as lung cancer. Its frequent expression in tumor cells but not in normal tissues makes MAGEA4 a target for cancer immunotherapy. Additionally studies have shown that MAGEA4 may interfere with the immune system's ability to detect and destroy cancerous cells partly due to interactions with proteins like CTLA-4 further emphasizing its potential as a therapeutic target in oncology.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAGEA4 antibody [CPTC-MAGEA4-1] - BSA and Azide free (ab268201), expandable thumbnail](https://content.abcam.com/products/images/magea4-antibody-cptc-magea4-1-bsa-and-azide-free-ab268201--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img113159.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAGEA4 antibody [CPTC-MAGEA4-1] - BSA and Azide free (ab268201)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAGEA4 antibody [CPTC-MAGEA4-1] - BSA and Azide free (ab268201)
Formalin-fixed, paraffin-embedded human testis tissue stained for MAGEA4 using Anti-MAGEA4 antibody [CPTC-MAGEA4-1] ab268080 at 2 μg/ml in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (Anti-MAGEA4 antibody [CPTC-MAGEA4-1] ab268080)
![Protein Array - Anti-MAGEA4 antibody [CPTC-MAGEA4-1] - BSA and Azide free (ab268201), expandable thumbnail](https://content.abcam.com/products/images/magea4-antibody-cptc-magea4-1-bsa-and-azide-free-ab268201--protein-array-img1219.jpg "Protein Array - Anti-MAGEA4 antibody [CPTC-MAGEA4-1] - BSA and Azide free (ab268201)")
Protein Array - Anti-MAGEA4 antibody [CPTC-MAGEA4-1] - BSA and Azide free (ab268201)
Protein Array containing more than 19,000 full-length human proteins using Anti-MAGEA4 antibody [CPTC-MAGEA4-1] ab268080.
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (Anti-MAGEA4 antibody [CPTC-MAGEA4-1] ab268080)
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