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Rabbit Recombinant Monoclonal MAGEA4 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAGEA4 antibody [EPR26098-164] - BSA and Azide free (AB323811), expandable thumbnail
  • Western blot - Anti-MAGEA4 antibody [EPR26098-164] - BSA and Azide free (AB323811), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAGEA4 antibody [EPR26098-164] - BSA and Azide free (AB323811), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAGEA4 antibody [EPR26098-164] - BSA and Azide free (AB323811), expandable thumbnail
  • Western blot - Anti-MAGEA4 antibody [EPR26098-164] - BSA and Azide free (AB323811), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWB
Human
Tested
Tested

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Target data

Function

Regulates cell proliferation through the inhibition of cell cycle arrest at the G1 phase (PubMed:22842486). Also negatively regulates p53-mediated apoptosis (PubMed:22842486).

Alternative names

MAGE4, MAGEA4, Melanoma-associated antigen 4, Cancer/testis antigen 1.4, MAGE-4 antigen, MAGE-41 antigen, MAGE-X2 antigen, CT1.4

Rabbit Recombinant Monoclonal MAGEA4 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR26098-164
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

ab323811 is the carrier free version of Anti-MAGEA4 antibody [EPR26098-164] ab323802.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAGEA4 antibody [EPR26098-164] - BSA and Azide free (ab323811), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAGEA4 antibody [EPR26098-164] - BSA and Azide free (ab323811)

    MAGEA4 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Human pancreas tissue using rabbit Anti-MAGEA4 antibody

    This data was developed using Anti-MAGEA4 antibody [EPR26098-164] ab323802, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling MAGEA4 with Anti-MAGEA4 antibody [EPR26098-164] ab323802 at 1/1000 (0.489 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Negative control: no staining on human pancreas. The primary antibody was incubated for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Western blot - Anti-MAGEA4 antibody [EPR26098-164] - BSA and Azide free (ab323811), expandable thumbnail

    Western blot - Anti-MAGEA4 antibody [EPR26098-164] - BSA and Azide free (ab323811)

    MAGEA4 Western blot staining using rabbit Anti-MAGEA4 antibody

    This data was developed using Anti-MAGEA4 antibody [EPR26098-164] ab323802, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This antibody does not cross-react with recombinant human MAGEA1, MAGEA3, MAGEA8, MAGEA6, MAGEA12 or MAGEA2 by western blot.

    In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.

    All lanes: Western blot - Anti-MAGEA4 antibody [EPR26098-164] (Anti-MAGEA4 antibody [EPR26098-164] ab323802) at 1/1000 dilution

    Lane 1: His-tagged human MAGEA4 recombinant protein at 10 ng

    Lane 2: His-tagged human MAGEA1 recombinant protein at 10 ng

    Lane 3: His-tagged human MAGEA3 recombinant protein at 10 ng

    Lane 4: His-tagged human MAGEA8 recombinant protein at 10 ng

    Lane 5: His-tagged human MAGEA6 recombinant protein at 10 ng

    Lane 6: His-tagged human MAGEA12 recombinant protein at 10 ng

    Lane 7: His-tagged human MAGEA2 recombinant protein at 10 ng

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 50 kDa, 50, 48, 49, 50, 48 kDa, 75, 73 kDa

    Exposure time: 37s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAGEA4 antibody [EPR26098-164] - BSA and Azide free (ab323811), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAGEA4 antibody [EPR26098-164] - BSA and Azide free (ab323811)

    MAGEA4 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Human lung carcinoma tissue using rabbit Anti-MAGEA4 antibody

    This data was developed using Anti-MAGEA4 antibody [EPR26098-164] ab323802, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labeling MAGEA4 with Anti-MAGEA4 antibody [EPR26098-164] ab323802 at 1/1000 (0.489 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on human lung carcinoma (PMID: 29901069). The primary antibody was incubated for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAGEA4 antibody [EPR26098-164] - BSA and Azide free (ab323811), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAGEA4 antibody [EPR26098-164] - BSA and Azide free (ab323811)

    MAGEA4 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Human testis tissue using rabbit Anti-MAGEA4 antibody

    This data was developed using Anti-MAGEA4 antibody [EPR26098-164] ab323802, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling MAGEA4 with Anti-MAGEA4 antibody [EPR26098-164] ab323802 at 1/1000 (0.489 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on human testis (PMID: 28105158). The primary antibody was incubated for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Western blot - Anti-MAGEA4 antibody [EPR26098-164] - BSA and Azide free (ab323811), expandable thumbnail

    Western blot - Anti-MAGEA4 antibody [EPR26098-164] - BSA and Azide free (ab323811)

    MAGEA4 Western blot staining using rabbit Anti-MAGEA4 antibody

    This data was developed using Anti-MAGEA4 antibody [EPR26098-164] ab323802, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Negative control: pancreas.

    In lanes 1-2 and 4, the lysates were stored at -80degC prior to Western Blotting. The bands beneath the target band (39 kDa) are likely to be degradation products. In lane 3, to minimize protein degradation, cells were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

    Lanes 1-2 are applied with Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 and lanes 3-4 are applied with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    Exposure time: Lanes 1-2: 180 seconds; Lanes 3-4: 26 seconds.

    All lanes: Western blot - Anti-MAGEA4 antibody [EPR26098-164] (Anti-MAGEA4 antibody [EPR26098-164] ab323802) at 1/1000 dilution

    Lane 1: Human testis tissue lysate (frozen) at 20 µg

    Lane 2: Human pancreas tissue lysate (frozen) at 20 µg

    Lane 3: NCI-H1299 (human lung carcinoma epithelial cell) whole cell lysate (fresh) at 20 µg

    Lane 4: NCI-H1299 (human lung carcinoma epithelial cell) whole cell lysate (frozen) at 20 µg

    Secondary

    Lanes 1 - 2: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Lanes 3 - 4: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 39 kDa, 36 kDa

  • Western blot - Anti-MAGEA4 antibody [EPR26098-164] - BSA and Azide free (ab323811), expandable thumbnail

    Western blot - Anti-MAGEA4 antibody [EPR26098-164] - BSA and Azide free (ab323811)

    MAGEA4 Western blot staining using rabbit Anti-MAGEA4 antibody

    This data was developed using Anti-MAGEA4 antibody [EPR26098-164] ab323802, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    To minimize protein degradation, cells were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

    Exposure time: Lane 1: 15 seconds; Lane 2: 125 seconds.

    All lanes: Western blot - Anti-MAGEA4 antibody [EPR26098-164] (Anti-MAGEA4 antibody [EPR26098-164] ab323802) at 1/1000 dilution

    Lane 1: NCI-H1299 (human lung carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: A431 (human epidermoid carcinoma epithelial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 39 kDa

  • Western blot - Anti-MAGEA4 antibody [EPR26098-164] - BSA and Azide free (ab323811), expandable thumbnail

    Western blot - Anti-MAGEA4 antibody [EPR26098-164] - BSA and Azide free (ab323811)

    MAGEA4 Western blot staining using rabbit Anti-MAGEA4 antibody

    This data was developed using Anti-MAGEA4 antibody [EPR26098-164] ab323802, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Negative control: MCF7, Daudi.

    To minimize protein degradation, cells were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-MAGEA4 antibody [EPR26098-164] (Anti-MAGEA4 antibody [EPR26098-164] ab323802) at 1/1000 dilution

    Lane 1: U-2 OS (human bone osteosarcoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 3: Daudi (human burkitt's lymphoma lymphoblast) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 39 kDa, 36 kDa

    Exposure time: 180s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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