Knockout Tested Mouse Monoclonal MAGEA4 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human MAGEA4.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAGEA4 antibody [OTI1F9] (AB139297), expandable thumbnail](https://content.abcam.com/products/images/magea4-antibody-oti1f9-ab139297--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img101353.gif "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAGEA4 antibody [OTI1F9] (AB139297)")
![Western blot - Anti-MAGEA4 antibody [OTI1F9] (AB139297), expandable thumbnail](https://content.abcam.com/products/images/magea4-antibody-oti1f9-ab139297--western-blot-img153656.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-MAGEA4 antibody [OTI1F9] (AB139297)")
![Western blot - Anti-MAGEA4 antibody [OTI1F9] (AB139297), expandable thumbnail](https://content.abcam.com/products/images/magea4-antibody-oti1f9-ab139297--western-blot-img418304.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-MAGEA4 antibody [OTI1F9] (AB139297)")
Publications
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- Journal of cancer research and clinical oncology 149:15003-150112023Expression of four cancer-testis antigens in TNBC indicating potential universal immunotherapeutic targets.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJie Xiao,Fengli Huang,Lin Li,Lianru Zhang,Li Xie,Baorui LiuPubMed 37610673
- International journal of molecular sciences 24:2023Target Selection for T-Cell Therapy in Epithelial Ovarian Cancer: Systematic Prioritization of Self-Antigens.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPaul Schossig,Ebru Coskun,Ruza Arsenic,David Horst,Jalid Sehouli,Eva Bergmann,Nadine Andresen,Christian Sigler,Antonia Busse,Ulrich Keller,Sebastian OchsenreitherPubMed 36768616
- Fertility and sterility 116:713-7202021Z-scores for comparative analyses of spermatogonial numbers throughout human development.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMiriam Funke,Yifan Yang,Atte Lahtinen,Klara Benninghoven-Frey,Sabine Kliesch,Nina Neuhaus,Jan-Bernd Stukenborg,Kirsi JahnukainenPubMed 33975728
- International journal of molecular sciences 22:2021Characterization of Estrogenic Activity and Site-Specific Accumulation of Bisphenol-A in Epididymal Fat Pad: Interfering Effects on the Endocannabinoid System and Temporal Progression of Germ Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTeresa Chioccarelli,Marina Migliaccio,Antonio Suglia,Francesco Manfrevola,Veronica Porreca,Nadia Diano,Sonia Errico,Silvia Fasano,Gilda CobellisPubMed 33802611
- Oncology letters 19:3982-39922020Myeloid-derived suppressor cells infiltration in non-small-cell lung cancer tumor and MAGE-A4 and NY-ESO-1 expression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhenbo Hou et. al.PubMed 32382343
- Human pathology 76:91-992018Spectrum of findings in orchiectomy specimens of persons undergoing gender confirmation surgery.
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human MAGEA4. Database link P43358
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/4000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/150 | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
Regulates cell proliferation through the inhibition of cell cycle arrest at the G1 phase (PubMed:22842486). Also negatively regulates p53-mediated apoptosis (PubMed:22842486).
Alternative names
MAGE4, MAGEA4, Melanoma-associated antigen 4, Cancer/testis antigen 1.4, MAGE-4 antigen, MAGE-41 antigen, MAGE-X2 antigen, CT1.4
Recommended products
Knockout Tested Mouse Monoclonal MAGEA4 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human MAGEA4.
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human MAGEA4. Database link P43358
- Clone number
- OTI1F9
- Purification technique
- Affinity purification Protein A/G
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Clone OTI1F9 (formerly 1F9).
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MAGEA4 also known as MAGE-A4 is part of the melanoma-associated antigen family. This protein is encoded by the MAGEA4 gene and is characterized by a molecular mass of approximately 42 kDa. Its expression is limited to certain tissues including the testis and is often silent in normal somatic tissues. However MAGEA4 is frequently found in various types of tumors making it a target of interest for cancer research and therapy.
- Biological function summary
MAGEA4 participates in cellular processes related to cancer progression. Although not part of a multi-protein complex its presence in tumorous tissues suggests a role in modulating cellular mechanisms that favor tumor survival and proliferation. MAGEA4 does not directly interact with other proteins but might affect cellular pathways indirectly contributing to oncogenesis.
- Pathways
MAGEA4 influences several cellular mechanisms that are related to cancer development. While it is not a central player in major biological pathways its function relates to processes affecting cell cycle regulation and apoptosis. This connection was observed in studies showing interactions with other cancer-related proteins like p53 suggesting that MAGEA4 can modify responses to apoptotic signals and may interact with or affect the TP53 pathway.
- Associated diseases and disorders
MAGEA4 is prominently linked to various cancers including melanomas and carcinomas such as lung cancer. Its frequent expression in tumor cells but not in normal tissues makes MAGEA4 a target for cancer immunotherapy. Additionally studies have shown that MAGEA4 may interfere with the immune system's ability to detect and destroy cancerous cells partly due to interactions with proteins like CTLA-4 further emphasizing its potential as a therapeutic target in oncology.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAGEA4 antibody [OTI1F9] (ab139297)
Paraffin-embedded human bladder carcinoma tissue stained for MAGEA4 using ab139297 at 1/150 dilution in immunohistochemical analysis.
![Western blot - Anti-MAGEA4 antibody [OTI1F9] (ab139297), expandable thumbnail](https://content.abcam.com/products/images/magea4-antibody-oti1f9-ab139297--western-blot-img153656.jpg "Western blot - Anti-MAGEA4 antibody [OTI1F9] (ab139297)")
Western blot - Anti-MAGEA4 antibody [OTI1F9] (ab139297)
All lanes: Western blot - Anti-MAGEA4 antibody [OTI1F9] (ab139297) at 1/4000 dilution
Lane 1: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control at 5 µg
Lane 2: HEK-293T cell lysate transfected with pCMV6-ENTRY MAGEA4 cDNA at 5 µg
Predicted band size: 35 kDa
![Western blot - Anti-MAGEA4 antibody [OTI1F9] (ab139297), expandable thumbnail](https://content.abcam.com/products/images/magea4-antibody-oti1f9-ab139297--western-blot-img418304.jpg "Western blot - Anti-MAGEA4 antibody [OTI1F9] (ab139297)")
Western blot - Anti-MAGEA4 antibody [OTI1F9] (ab139297)
False colour image of Western blot: Anti-MAGEA4 antibody [OTI1F9] staining at 1/4000 dilution, shown in green; Rabbit anti-alpha Tubulin antibody [EP1332Y] (Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker ab52866) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab139297 was shown to bind specifically to MAGEA4. A band was observed at 40 kDa in wild-type A431 cell lysates with no signal observed at this size in MAGEA4 knockout cell line Human MAGEA4 knockout A-431 cell line ab273857 (knockout cell lysate ab273811). To generate this image, wild-type and MAGEA4 knockout A431 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L 800CW and Goat anti-Rabbit IgG H&L 680RD at 1/20000 dilution.
All lanes: Western blot - Anti-MAGEA4 antibody [OTI1F9] (ab139297) at 1/4000 dilution
Lane 1: Wild-type A431 cell lysate at 20 µg
Lane 2: Western blot - Human MAGEA4 knockout A-431 cell lysate (ab273811) at 20 µg
Lane 3: U-2 OS cell lysate at 20 µg
Lane 4: SH-SY5Y cell lysate at 20 µg
SecondaryAll lanes: Goat anti-Mouse IgG H&L 800CW and Goat anti-Rabbit IgG H&L 680RD at 1/20000 dilution
Performed under reducing conditions.
Predicted band size: 35 kDa
Observed band size: 40 kDa
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