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AB250716

Anti-MAGMAS antibody [EPR15214] - BSA and Azide free

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Rabbit Recombinant Monoclonal MAGMAS antibody. Carrier free. Suitable for ICC/IF, WB, IHC-P and reacts with Human, Mouse samples.

View Alternative Names

MAGMAS, TIM16, TIMM16, CGI-136, PAM16, Mitochondrial import inner membrane translocase subunit TIM16, Mitochondria-associated granulocyte macrophage CSF-signaling molecule, Presequence translocated-associated motor subunit PAM16

5 Images
Immunocytochemistry/ Immunofluorescence - Anti-MAGMAS antibody [EPR15214] - BSA and Azide free (AB250716)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MAGMAS antibody [EPR15214] - BSA and Azide free (AB250716)

This data was developed using ab184157, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% paraformaldehyde-fixed HepG2 cells labeling MAGMAS with ab184157 at 1/250 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody at 1/200 dilution. Counter stained with DAPI (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAGMAS antibody [EPR15214] - BSA and Azide free (AB250716)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAGMAS antibody [EPR15214] - BSA and Azide free (AB250716)

This data was developed using ab184157, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling MAGMAS with ab184157 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained using hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAGMAS antibody [EPR15214] - BSA and Azide free (AB250716)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAGMAS antibody [EPR15214] - BSA and Azide free (AB250716)

This data was developed using ab184157, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue labeling MAGMAS with ab184157 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained using hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Western blot - Anti-MAGMAS antibody [EPR15214] - BSA and Azide free (AB250716)
  • WB

Supplier Data

Western blot - Anti-MAGMAS antibody [EPR15214] - BSA and Azide free (AB250716)

This data was developed using ab184157, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-MAGMAS antibody [EPR15214] (<a href='/en-us/products/primary-antibodies/magmas-antibody-epr15214-ab184157'>ab184157</a>) at 1/20000 dilution

All lanes:

Human skeletal muscle tissue lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 14 kDa

Observed band size: 13 kDa

false

Western blot - Anti-MAGMAS antibody [EPR15214] - BSA and Azide free (AB250716)
  • WB

Supplier Data

Western blot - Anti-MAGMAS antibody [EPR15214] - BSA and Azide free (AB250716)

This data was developed using ab184157, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-MAGMAS antibody [EPR15214] (<a href='/en-us/products/primary-antibodies/magmas-antibody-epr15214-ab184157'>ab184157</a>) at 1/20000 dilution

Lane 1:

HepG2 cell lysate at 20 µg

Lane 2:

LnCaP cell lysate at 20 µg

Lane 3:

Jurkat cell lysate at 20 µg

Secondary

All lanes:

Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

Predicted band size: 14 kDa

Observed band size: 13 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15214

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

ab250716 is the carrier-free version of ab184157.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Regulates ATP-dependent protein translocation into the mitochondrial matrix. Inhibits DNAJC19 stimulation of HSPA9/Mortalin ATPase activity.
See full target information PAM16
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com