AB193983

Magnetic beads Anti-GFP VHH Single Domain antibody

Recombinant
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(8 Publications)

Llama Recombinant Monoclonal GFP antibody - conjugated to Magnetic beads. Suitable for IP.

See more GFP antibodies and assay kits

View Alternative Names

Green fluorescent protein, GFP

3 Images

Lab

Immunoprecipitation - Magnetic beads Anti-GFP VHH Single Domain antibody (AB193983)

Lane 1 : HEK293 cell lysate overexpressing GFP - 10ug

Lane 2 : Unbound IP supernatant : Anti-GFP antibody (Magnetic beads) + 100ug HEK293 cell lysate overexpressing GFP

Lane 3 : Negative IP : off-target sdAb beads : Anti-GFP antibody (Magnetic beads) + 100ug HEK293 cell lysate overexpressing GFP

Lane 4 : IP : Anti-GFP antibody (Magnetic beads) + 100ug HEK293 cell lysate overexpressing GFP

Lane 5 : HEK293 cell lysate overexpressing GFP - 10ug

Lane 6 : Unbound IP supernatant : anti-GFP antibody (ab290) + Protein A agarose + 100ug HEK293 cell lysate overexpressing GFP

Lane 7 : IP : anti-GFP antibody (ab290) + Protein A agarose+ 100ug HEK293 cell lysate overexpressing GFP

Anti-GFP antibody (Magnetic beads) (ab193983) was used to pull down GFP from HEK293 cell lysate overexpressing GFP. ab290 (Rabbit polyclonal to GFP - ChIP Grade) was used as a positive control. The gel was stained with Optiblot Blue (ab119211).

All lanes:

Immunoprecipitation - Magnetic beads Anti-GFP VHH Single Domain antibody (ab193983) at 1/500 dilution

Secondary

All lanes:

Immunoprecipitation - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-h-l-hrp-preadsorbed-ab97040'>ab97040</a>) at 1/20000 dilution

Observed band size: 48 kDa

false

Lab

Immunoprecipitation - Magnetic beads Anti-GFP VHH Single Domain antibody (AB193983)

Lane 1 : HEK293 cell lysate overexpressing GFP - 10ug

Lane 2 : Unbound IP supernatant : Anti-GFP antibody (Magnetic beads) + 100ug HEK293 cell lysate overexpressing GFP

Lane 3 : Negative IP : off-target sdAb beads : Anti-GFP antibody (Magnetic beads) + 100ug HEK293 cell lysate overexpressing GFP

Lane 4 : IP : Anti-GFP antibody (Magnetic beads) + 100ug HEK293 cell lysate overexpressing GFP

Lane 5 : HEK293 cell lysate overexpressing GFP - 10ug

Lane 6 : Unbound IP supernatant : anti-GFP antibody (ab290) + Protein A agarose + 100ug HEK293 cell lysate overexpressing GFP

Lane 7 : IP : anti-GFP antibody (ab290) + Protein A agarose+ 100ug HEK293 cell lysate overexpressing GFP

All lanes:

Immunoprecipitation - Magnetic beads Anti-GFP VHH Single Domain antibody (ab193983) at 1/500 dilution

All lanes:

HEK293 whole cell lysate - Overexpressing GFP at 10 µg

Secondary

All lanes:

Immunoprecipitation - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-h-l-hrp-preadsorbed-ab97040'>ab97040</a>) at 1/20000 dilution

Observed band size: 48 kDa

false

Exposure time: 30s

Lab

Immunoprecipitation - Magnetic beads Anti-GFP VHH Single Domain antibody (AB193983)

Lane 1 : Anti-GFP antibody (Magnetic beads) (ab193983) + 10ul recombinant GFP (ab84191)

Lane 2 : Anti-GFP antibody (Magnetic beads) (ab193983) + 10ul GFP fusion protein.

Lane 3 : Off-target sdAb beads : Anti-Rabbit VHH Single Domain Antibody (Magnetic) + 10ul recombinant GFP (ab84191)

Lane 4 : Anti-GFP antibody (ab290) + Protein A agarose + 10ul recombinant GFP (ab84191)

Lane 5 : Anti-GFP antibody (ab290) + Protein A agarose + 10ul fusion GFP

Anti-GFP antibody (Magnetic beads) (ab193983) (20ul slurry) was used to pull down recombinant GFP (ab84191) and GFP fusion protein. ab290 (Rabbit polyclonal to GFP - ChIP Grade) and Protein A agarose was used as a positive control. The gel was stained with Optiblot Blue (ab119211).

Lanes 1 - 2:

Immunoprecipitation - Magnetic beads Anti-GFP VHH Single Domain antibody (ab193983) at 20 µL

Lane 3:

Immunoprecipitation - Magnetic beads Anti-GFP VHH Single Domain antibody (ab193983)

Lanes 4 - 5:

Immunoprecipitation - Magnetic beads Anti-GFP VHH Single Domain antibody (ab193983) at 1/500 dilution

Secondary

All lanes:

Immunoprecipitation - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-h-l-hrp-preadsorbed-ab97040'>ab97040</a>) at 1/20000 dilution

Observed band size: 27 kDa

false

Exposure time: 30s

Key facts

Host species

Llama

Clonality

Monoclonal

Clone number

GD002

Isotype

VHH

Conjugation

Magnetic beads

Excitation/Emission

Carrier free

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Recombinant full length protein - Aequorea victoria": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 2.92% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.

See full target information GFP

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:21604 PubMed40594474

2025

The novel amino-artemisinin derivative WHN-11 disrupts mitochondria and protein homeostasis, and induces autophagy and apoptosis in cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Deborah Kajewole,Ho Ning Wong,Alexander von Kriegsheim,Richard K Haynes,Jo-Anne de la Mare,Adrienne Lesley Edkins

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2404067 PubMed39373352

2024

CYLD Maintains Retinal Homeostasis by Deubiquitinating ENKD1 and Promoting the Phagocytosis of Photoreceptor Outer Segments.

Applications

Unspecified application

Species

Unspecified reactive species

Song Yang,Fan Yu,Mulin Yang,Hua Ni,Weiwen Bu,Hanxiao Yin,Jia Yang,Weishu Wang,Denghui Zhai,Xuemei Wu,Nan Ma,Te Li,Huijie Hao,Jie Ran,Ting Song,Dengwen Li,Sei Yoshida,Quanlong Lu,Yunfan Yang,Jun Zhou,Min Liu

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 10:e2303545 PubMed37963851

2023

O-GlcNAcylation Regulates Centrosome Behavior and Cell Polarity to Reduce Pulmonary Fibrosis and Maintain the Epithelial Phenotype.

Applications

Unspecified application

Species

Unspecified reactive species

Fan Yu,Song Yang,Hua Ni,Dai Heng,Xuemei Wu,Mulin Yang,Xinming Zhang,Yuxin Cao,Yandong Pei,Di An,Dengwen Li,Dayong Liu,Lin Liu,Leiting Pan,Quan Chen,Xueliang Zhu,Jun Zhou

International journal of molecular sciences 24: PubMed37372954

2023

p53 Affects Zeb1 Interactome of Breast Cancer Stem Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Sergey E Parfenyev,Sergey V Shabelnikov,Elena N Tolkunova,Nickolai A Barlev,Alexey G Mittenberg

Molecular medicine reports 26: PubMed35920180

2022

LncRNA GAS5 promotes spermidine‑induced autophagy through the miRNA‑31‑5p/NAT8L axis in pulmonary artery endothelial cells of patients with CTEPH.

Applications

Unspecified application

Species

Unspecified reactive species

Qinghua Wu,Xiaohui Zhou,Yan Wang,Yamin Hu

Cell discovery 8:40 PubMed35504898

2022

Dynamic O-GlcNAcylation coordinates ferritinophagy and mitophagy to activate ferroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Fan Yu,Qianping Zhang,Hanyu Liu,Jinming Liu,Song Yang,Xiaofan Luo,Wei Liu,Hao Zheng,Qiqi Liu,Yunxi Cui,Guo Chen,Yanjun Li,Xinglu Huang,Xiyun Yan,Jun Zhou,Quan Chen

Neuron 109:1825-1835.e5 PubMed33887199

2021

Translation of GGC repeat expansions into a toxic polyglycine protein in NIID defines a novel class of human genetic disorders: The polyG diseases.

Applications

Unspecified application

Species

Unspecified reactive species

Manon Boivin,Jianwen Deng,Véronique Pfister,Erwan Grandgirard,Mustapha Oulad-Abdelghani,Bastien Morlet,Frank Ruffenach,Luc Negroni,Pascale Koebel,Hugues Jacob,Fabrice Riet,Anke A Dijkstra,Kathryn McFadden,Wiley A Clayton,Daojun Hong,Hiroaki Miyahara,Yasushi Iwasaki,Jun Sone,Zhaoxia Wang,Nicolas Charlet-Berguerand

Neuron 91:41-55 PubMed27321923

2016

Activation of HIPK2 Promotes ER Stress-Mediated Neurodegeneration in Amyotrophic Lateral Sclerosis.

Applications

Species

Unspecified reactive species

Sebum Lee,Yulei Shang,Stephanie A Redmond,Anatoly Urisman,Amy A Tang,Kathy H Li,Alma L Burlingame,Ryan A Pak,Ana Jovičić,Aaron D Gitler,Jinhua Wang,Nathanael S Gray,William W Seeley,Teepu Siddique,Eileen H Bigio,Virginia M-Y Lee,John Q Trojanowski,Jonah R Chan,Eric J Huang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Magnetic beads Anti-GFP VHH Single Domain antibody

Immunoprecipitation - Magnetic beads Anti-GFP VHH Single Domain antibody (AB193983)

Immunoprecipitation - Magnetic beads Anti-GFP VHH Single Domain antibody (AB193983)

Immunoprecipitation - Magnetic beads Anti-GFP VHH Single Domain antibody (AB193983)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (8)

Product promise