JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB186431

Anti-Mago nashi homolog 2 antibody [EPR14037-43]

Be the first to review this product! Submit a review

|

(3 Publications)

Rabbit Recombinant Monoclonal Mago nashi homolog 2 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 3 publications.

View Alternative Names

MAGOH2, MAGOHB, Protein mago nashi homolog 2

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mago nashi homolog 2 antibody [EPR14037-43] (AB186431)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mago nashi homolog 2 antibody [EPR14037-43] (AB186431)

Immunohistochemical analysis of paraffin-embedded Human prostatic hyperplasia tissue labeling Mago nashi homolog 2 with ab186431 at 1/1000 dilution. Counter stain Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mago nashi homolog 2 antibody [EPR14037-43] (AB186431)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mago nashi homolog 2 antibody [EPR14037-43] (AB186431)

Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling Mago nashi homolog 2 with ab186431 at 1/1000 dilution. Counter stain Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-Mago nashi homolog 2 antibody [EPR14037-43] (AB186431)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Mago nashi homolog 2 antibody [EPR14037-43] (AB186431)

Immunofluorescent analysis of 4% paraformaldehyde fixed HeLa cells labeling Mago nashi homolog 2 with ab186431 at 1/250 dilution. Secondary antibody Goat anti rabbit IgG (Alexa Fluor®555) at a 1/200 dilution. Counterstained with Dapi blue.

Western blot - Anti-Mago nashi homolog 2 antibody [EPR14037-43] (AB186431)
  • WB

Supplier Data

Western blot - Anti-Mago nashi homolog 2 antibody [EPR14037-43] (AB186431)

All lanes:

Western blot - Anti-Mago nashi homolog 2 antibody [EPR14037-43] (ab186431) at 1/20000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

Jurkat cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 17 kDa

Observed band size: 17 kDa

false

Western blot - Anti-Mago nashi homolog 2 antibody [EPR14037-43] (AB186431)
  • WB

Supplier Data

Western blot - Anti-Mago nashi homolog 2 antibody [EPR14037-43] (AB186431)

All lanes:

Western blot - Anti-Mago nashi homolog 2 antibody [EPR14037-43] (ab186431) at 1/10000 dilution

Lane 1:

Raw264.7 cell lysate at 10 µg

Lane 2:

PC12 cell lysate at 10 µg

Lane 3:

NIH/3T3 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

false

  • Carrier free

    Anti-Mago nashi homolog 2 antibody [EPR14037-43] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14037-43

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The immunogen shares 100% homology with both MAGOHB (UniProt Q96A72) and MAGOH (UniProt P61326). We have not performed any tests in order to determine the cross-reactivity but it is possible that ab186431 could cross-react with both MAGOHB and MAGOH. Both proteins have a similar molecular weight of 17 kDa.

style
left-column

Complementary Products

Primary Antibodies

AB170944

Anti-MAGOH antibody [EPR10745]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAGOH antibody [EPR10745] (AB170944)

  • 0
  • 0
Primary Antibodies

AB290734

Anti-YTHDF1 + YTHDF3 + YTHDF2 antibody [EPR26183-69]

20ul selling size|RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-YTHDF1 + YTHDF3 + YTHDF2 antibody [EPR26183-69] (AB290734)

  • 0
  • 0
Primary Antibodies

AB32485

Anti-eIF4A3 antibody

Immunocytochemistry/ Immunofluorescence - Anti-eIF4A3 antibody (AB32485)

  • 4
  • 9
Primary Antibodies

AB282457

Anti-EpCAM antibody [RM1016]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunocytochemistry - Anti-EpCAM antibody [RM1016] (AB282457)

  • 3
  • 1
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Conditional Ambient
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Mago nashi homolog 2 often referred to as MAGOH2 is a protein with a molecular mass of approximately 18 kilodaltons. It is part of a conserved family of proteins with the notable family member being Mago nashi homolog 1 (MAGOH1). MAGOH2 is ubiquitously expressed in various tissues indicating its wide biological significance. The protein operates mainly in the cell nucleus where it forms important part of ribonucleoprotein complexes.
Biological function summary

MAGOH2 plays a significant role in RNA processing and development. It is part of the exon junction complex which influences mRNA splicing export and surveillance. This protein interacts closely with Y14 and other factors to regulate stable mRNA transport and nonsense-mediated decay. By being part of this complex MAGOH2 also aids in controlling gene expression critical for cell proliferation and differentiation.

Pathways

MAGOH2 functions in the mRNA surveillance and splicing pathways. It collaborates with related proteins such as Y14 in the exon junction complex to ensure proper gene expression post-transcription. The exon junction complex affects mRNA stability and quality control which are essential for the fidelity of genetic information. These pathways highlight the importance of MAGOH2 in maintaining efficient gene expression processes.

Altered function or expression of MAGOH2 can relate to developmental disorders and cancers. Abnormal exon junction complex function involving MAGOH2 and Y14 may disrupt normal RNA processing affecting normal cellular development and differentiation. The study of MAGOH2 and its interactions within the exon junction complex provides insights into congenital disorders and tumorigenesis processes. By exploring these connections researchers can better understand MAGOH2's role in health and disease contexts.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Required for pre-mRNA splicing as component of the spliceosome (PubMed : 28502770, PubMed : 29301961, PubMed : 30705154). Plays a redundant role with MAGOH in the exon junction complex and in the nonsense-mediated decay (NMD) pathway (PubMed : 23917022).
See full target information MAGOHB
style
left-column

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 13:7904 PubMed36550132

2022

Exon junction complex shapes the mA epitranscriptome.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Yang,Robinson Triboulet,Qi Liu,Erdem Sendinc,Richard I Gregory

RNA (New York, N.Y.) 29:55-68 PubMed36316088

2022

The kinesin motor KIF1C is a putative transporter of the exon junction complex in neuronal cells.

Applications

Unspecified application

Species

Unspecified reactive species

Maike Nagel,Marvin Noß,Jishu Xu,Nicola Horn,Marius Ueffing,Karsten Boldt,Rebecca Schüle

Life science alliance 1:e201800070 PubMed30456350

2018

Proteomics and neuropathology identify ribosomes as poly-GR/PR interactors driving toxicity.

Applications

Unspecified application

Species

Unspecified reactive species

Hannelore Hartmann,Daniel Hornburg,Mareike Czuppa,Jakob Bader,Meike Michaelsen,Daniel Farny,Thomas Arzberger,Matthias Mann,Felix Meissner,Dieter Edbauer
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com