AB180505

Anti-MAGOH+MAGOHB antibody [EPR14037]

Name: Anti-MAGOH+MAGOHB antibody [EPR14037] (ab180505) | Abcam
Brand: Abcam Limited
SKU: AB180505
Price: 510 USD
Availability: InStock
Rating: 5 (5 reviews)

RabMAb
Recombinant
What is this?

(5 Reviews)

(2 Publications)

Rabbit Recombinant Monoclonal MAGOH antibody. Suitable for WB, IHC-P, Dot and reacts with Human, Recombinant fragment - Human samples. Cited in 2 publications.

View Alternative Names

MAGOHA, MAGOH, Protein mago nashi homolog, MAGOH2, MAGOHB, Protein mago nashi homolog 2

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAGOH+MAGOHB antibody [EPR14037] (AB180505)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAGOH+MAGOHB antibody [EPR14037] (AB180505)

Immunohistochemical analysis of paraffin-embedded Human endometrial adenocarcinoma tissue labeling Mago nashi homolog 2 with ab180505 at 1/250 dilution. Secondary ab : HRP Polymer for Rabbit IgG. Counter stain : Hematoxylin

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-MAGOH+MAGOHB antibody [EPR14037] (AB180505)

Blocking buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-MAGOH+MAGOHB antibody [EPR14037] (ab180505) at 1/2000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

Raji cell lysate at 20 µg

Lane 3:

Jurkat cell lysate at 20 µg

Lane 4:

HL60 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 17 kDa

Observed band size: 17 kDa

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Lab

Dot Blot - Anti-MAGOH+MAGOHB antibody [EPR14037] (AB180505)

Dot blot analysis of MAGOH+MAGOHB using ab180505 followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 100,000 dilution.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody cross-react with human MAGOH.

All lanes:

Dot Blot - Anti-MAGOH+MAGOHB antibody [EPR14037] (ab180505)

Lane 1:

Human MAGOHB protein

Lane 2:

Human MAGOH protein

Secondary

All lanes:

Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false

Exposure time: 180s

Carrier free

Anti-MAGOH+MAGOHB antibody [EPR14037] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14037

Isotype

IgG

Carrier free

Reacts with

Human

Applications

Dot, WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

ab180505 detects both Mago nashi homolog and Mago nashi homolog 2

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "" }, "Recombinant fragment - Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Required for pre-mRNA splicing as component of the spliceosome (PubMed : 11991638). Plays a redundant role with MAGOHB as core component of the exon junction complex (EJC) and in the nonsense-mediated decay (NMD) pathway (PubMed : 23917022). The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC marks the position of the exon-exon junction in the mature mRNA for the gene expression machinery and the core components remain bound to spliced mRNAs throughout all stages of mRNA metabolism thereby influencing downstream processes including nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). The MAGOH-RBM8A heterodimer inhibits the ATPase activity of EIF4A3, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. The MAGOH-RBM8A heterodimer interacts with the EJC key regulator PYM1 leading to EJC disassembly in the cytoplasm and translation enhancement of EJC-bearing spliced mRNAs by recruiting them to the ribosomal 48S pre-initiation complex. Involved in the splicing modulation of BCL2L1/Bcl-X (and probably other apoptotic genes); specifically inhibits formation of proapoptotic isoforms such as Bcl-X(S); the function is different from the established EJC assembly.

See full target information MAGOH

Additional targets

MAGOHB

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:21180 PubMed40596687

2025

Pan-cancer analysis of oncogenic role of MAGOH and experiment validation in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Zezhi Qiu,Yi Le,Yanyu Liao,Xiao Chen,Xing Xin,Fengting Nie,Xianpin Wei,Heng Wang,Ziling Fang

Molecular cell 72:496-509.e9 PubMed30388411

2018

Exon Junction Complex Shapes the Transcriptome by Repressing Recursive Splicing.

Applications

Unspecified application

Species

Unspecified reactive species

Lorea Blazquez,Warren Emmett,Rupert Faraway,Jose Mario Bello Pineda,Simon Bajew,Andre Gohr,Nejc Haberman,Christopher R Sibley,Robert K Bradley,Manuel Irimia,Jernej Ule

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MAGOH+MAGOHB antibody [EPR14037]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAGOH+MAGOHB antibody [EPR14037] (AB180505)

Western blot - Anti-MAGOH+MAGOHB antibody [EPR14037] (AB180505)

Dot Blot - Anti-MAGOH+MAGOHB antibody [EPR14037] (AB180505)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Alternative Products

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Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Additional targets

Publications (2)

Product promise