Anti-MAGP2 antibody [EPR17581]

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-MAGP2 antibody [EPR17581] (AB203828)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling MAGP2 with ab203828 at 1/750 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/500 dilution was used as the secondary antibody.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAGP2 antibody [EPR17581] (AB203828)

Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling MAGP2 with ab203828 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on Human breast carcinoma tissue is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAGP2 antibody [EPR17581] (AB203828)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling MAGP2 with ab203828 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on Human colon tissue is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IP

Supplier Data

Immunoprecipitation - Anti-MAGP2 antibody [EPR17581] (AB203828)

MAGP2 was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate with ab203828 at 1/200 dilution. Western blot was performed from the immunoprecipitate using ab203828 at 1/5000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500.

Lane 1 : HeLa whole cell lysate 10ug (Input). Lane 2 : ab203828 IP in HeLa whole cell lysate. Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab203828 in HeLa whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 seconds.

All lanes:

Immunoprecipitation - Anti-MAGP2 antibody [EPR17581] (ab203828)

Predicted band size: 20 kDa

false

• WB

Supplier Data

Western blot - Anti-MAGP2 antibody [EPR17581] (AB203828)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-MAGP2 antibody [EPR17581] (ab203828) at 1/10000 dilution

All lanes:

Human bladder lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 20 kDa

Observed band size: 25 kDa

false

Exposure time: 15s

• WB

Supplier Data

Western blot - Anti-MAGP2 antibody [EPR17581] (AB203828)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-MAGP2 antibody [EPR17581] (ab203828) at 1/5000 dilution

All lanes:

HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 20 kDa

Observed band size: 25 kDa

false

Exposure time: 15s

• WB

Supplier Data

Western blot - Anti-MAGP2 antibody [EPR17581] (AB203828)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-MAGP2 antibody [EPR17581] (ab203828) at 1/1000 dilution

Lane 1:

Rat kidney lysate at 10 µg

Lane 2:

Rat spleen lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 20 kDa

Observed band size: 25 kDa

false

Exposure time: 3min

• WB

CiteAb

Western blot - Anti-MAGP2 antibody [EPR17581] (AB203828)

MAGP2 western blot using anti-MAGP2 antibody [EPR17581] ab203828. Publication image and figure legend from Li, J. H., Zhu, X. X., et al., 2019, J Exp Clin Cancer Res, PubMed 31775892.

ab203828 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab203828 please see the product overview.

MFAP5 positively regulated Notch1 pathway transcription. a Heat map showed results of RNA-seq of SSP-25 and RBE transformed sh-control, sh-MFAP5–1# and sh-MFAP5–2#. b, c GSEA results showed down-regulation of Notch pathway in cells transfected sh-MFAP5 compared with sh-Control. d, e RT-PCR results showed the mRNA level of genes in NOTCH1 pathway after transfected sh-MFAP5 in ICC cell lines. f, g Western blot analysis of Notch1 pathway target genes in sh-MFAP5 and sh-Control cells (f) and co-culture with recMFAP5 and control cells (G, each "+" represented 50μg/ml recMFAP5). h, i Co-IP results showed that MFAP5 could bind directly with NOTCH1. *p < 0.05,**p < 0.01,***p<0.001

false