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AB279383

Anti-MAK antibody [OTI1G2]

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(1 Publication)

Mouse Monoclonal MAK antibody. Suitable for WB, IHC-P and reacts with Recombinant fragment - Human, Human, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human MAK aa 250 to C-terminus.

View Alternative Names

Serine/threonine-protein kinase MAK, Male germ cell-associated kinase, MAK

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAK antibody [OTI1G2] (AB279383)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAK antibody [OTI1G2] (AB279383)

Paraffin embedded human pancreas tissue stained for MAK using ab279383 at 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer pH8.5 at 120°C for 3minutes.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAK antibody [OTI1G2] (AB279383)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAK antibody [OTI1G2] (AB279383)

Paraffin embedded carcinoma of human thyroid tissue stained for MAK using ab279383 at 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer pH8.5 at 120°C for 3minutes.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAK antibody [OTI1G2] (AB279383)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAK antibody [OTI1G2] (AB279383)

Paraffin embedded carcinoma of human prostate tissue stained for MAK using ab279383 at 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer pH8.5 at 120°C for 3minutes.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAK antibody [OTI1G2] (AB279383)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAK antibody [OTI1G2] (AB279383)

Paraffin embedded human liver tissue stained for MAK using ab279383 at 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer pH8.5 at 120°C for 3minutes.

Western blot - Anti-MAK antibody [OTI1G2] (AB279383)
  • WB

Supplier Data

Western blot - Anti-MAK antibody [OTI1G2] (AB279383)

All lanes:

Western blot - Anti-MAK antibody [OTI1G2] (ab279383) at 1/500 dilution

Lane 1:

HEK-293T cell extract at 35 µg

Lane 2:

HeLa cell extract at 35 µg

Lane 3:

HepG2 cell extract at 35 µg

Predicted band size: 71 kDa

false

Western blot - Anti-MAK antibody [OTI1G2] (AB279383)
  • WB

Supplier Data

Western blot - Anti-MAK antibody [OTI1G2] (AB279383)

All lanes:

Western blot - Anti-MAK antibody [OTI1G2] (ab279383) at 1/2000 dilution

Lane 1:

HEK-293T cells transfected with the pCMV6-ENTRY control at 5 µg

Lane 2:

HEK-293T cells transfected with pCMV6-ENTRY MAK at 5 µg

Predicted band size: 71 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

OTI1G2

Isotype

IgG1

Carrier free

No

Reacts with

Rat, Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human MAK aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P20794

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/150", "IHCP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/150", "IHCP-species-notes": "<p></p>" }, "Recombinant fragment - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Essential for the regulation of ciliary length and required for the long-term survival of photoreceptors (By similarity). Phosphorylates FZR1 in a cell cycle-dependent manner. Plays a role in the transcriptional coactivation of AR. Could play an important function in spermatogenesis. May play a role in chromosomal stability in prostate cancer cells.
See full target information MAK
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of neurochemistry 169:e16218 PubMed39233334

2024

Visualization of myelin-forming oligodendrocytes in the adult mouse brain.

Applications

Unspecified application

Species

Unspecified reactive species

Kiichi Yokoyama,Yuichi Hiraoka,Yoshifumi Abe,Kenji F Tanaka
View all publications
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Product promise

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