Mouse Monoclonal MAK antibody. Suitable for WB, IHC-P and reacts with Recombinant fragment - Human, Human, Rat samples. Immunogen corresponding to Recombinant Fragment Protein within Human MAK aa 250 to C-terminus.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAK antibody [OTI1G2] (AB279383), expandable thumbnail](https://content.abcam.com/products/images/mak-antibody-oti1g2-ab279383--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img112281.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAK antibody [OTI1G2] (AB279383)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAK antibody [OTI1G2] (AB279383), expandable thumbnail](https://content.abcam.com/products/images/mak-antibody-oti1g2-ab279383--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img78009.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAK antibody [OTI1G2] (AB279383)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAK antibody [OTI1G2] (AB279383), expandable thumbnail](https://content.abcam.com/products/images/mak-antibody-oti1g2-ab279383--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img135221.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAK antibody [OTI1G2] (AB279383)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAK antibody [OTI1G2] (AB279383), expandable thumbnail](https://content.abcam.com/products/images/mak-antibody-oti1g2-ab279383--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img134778.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAK antibody [OTI1G2] (AB279383)")
![Western blot - Anti-MAK antibody [OTI1G2] (AB279383), expandable thumbnail](https://content.abcam.com/products/images/mak-antibody-oti1g2-ab279383--western-blot-img16729.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-MAK antibody [OTI1G2] (AB279383)")
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Fragment Protein within Human MAK aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P20794
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Rat | Expected | Expected |
| Recombinant fragment - Human | Tested | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info 1/500.00000 - 1/2000.00000 | Notes - |
Species Human | Dilution info 1/500.00000 - 1/2000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/500.00000 - 1/2000.00000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/150 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/150 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
Target data
Function
Essential for the regulation of ciliary length and required for the long-term survival of photoreceptors (By similarity). Phosphorylates FZR1 in a cell cycle-dependent manner. Plays a role in the transcriptional coactivation of AR. Could play an important function in spermatogenesis. May play a role in chromosomal stability in prostate cancer cells.
Alternative names
Serine/threonine-protein kinase MAK, Male germ cell-associated kinase, MAK
Recommended products
Mouse Monoclonal MAK antibody. Suitable for WB, IHC-P and reacts with Recombinant fragment - Human, Human, Rat samples. Immunogen corresponding to Recombinant Fragment Protein within Human MAK aa 250 to C-terminus.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Fragment Protein within Human MAK aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P20794
- Clone number
- OTI1G2
- Purification technique
- Affinity purification Protein A/G
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAK antibody [OTI1G2] (ab279383), expandable thumbnail](https://content.abcam.com/products/images/mak-antibody-oti1g2-ab279383--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img112281.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAK antibody [OTI1G2] (ab279383)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAK antibody [OTI1G2] (ab279383)
Paraffin embedded carcinoma of human prostate tissue stained for MAK using ab279383 at 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer pH8.5 at 120°C for 3minutes.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAK antibody [OTI1G2] (ab279383), expandable thumbnail](https://content.abcam.com/products/images/mak-antibody-oti1g2-ab279383--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img78009.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAK antibody [OTI1G2] (ab279383)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAK antibody [OTI1G2] (ab279383)
Paraffin embedded carcinoma of human thyroid tissue stained for MAK using ab279383 at 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer pH8.5 at 120°C for 3minutes.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAK antibody [OTI1G2] (ab279383), expandable thumbnail](https://content.abcam.com/products/images/mak-antibody-oti1g2-ab279383--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img135221.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAK antibody [OTI1G2] (ab279383)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAK antibody [OTI1G2] (ab279383)
Paraffin embedded human pancreas tissue stained for MAK using ab279383 at 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer pH8.5 at 120°C for 3minutes.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAK antibody [OTI1G2] (ab279383), expandable thumbnail](https://content.abcam.com/products/images/mak-antibody-oti1g2-ab279383--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img134778.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAK antibody [OTI1G2] (ab279383)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAK antibody [OTI1G2] (ab279383)
Paraffin embedded human liver tissue stained for MAK using ab279383 at 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer pH8.5 at 120°C for 3minutes.
![Western blot - Anti-MAK antibody [OTI1G2] (ab279383), expandable thumbnail](https://content.abcam.com/products/images/mak-antibody-oti1g2-ab279383--western-blot-img16729.jpg "Western blot - Anti-MAK antibody [OTI1G2] (ab279383)")
Western blot - Anti-MAK antibody [OTI1G2] (ab279383)
All lanes: Western blot - Anti-MAK antibody [OTI1G2] (ab279383) at 1/500 dilution
Lane 1: HEK-293T cell extract at 35 µg
Lane 2: HeLa cell extract at 35 µg
Lane 3: HepG2 cell extract at 35 µg
Predicted band size: 71 kDa
![Western blot - Anti-MAK antibody [OTI1G2] (ab279383), expandable thumbnail](https://content.abcam.com/products/images/mak-antibody-oti1g2-ab279383--western-blot-img152461.jpg "Western blot - Anti-MAK antibody [OTI1G2] (ab279383)")
Western blot - Anti-MAK antibody [OTI1G2] (ab279383)
All lanes: Western blot - Anti-MAK antibody [OTI1G2] (ab279383) at 1/2000 dilution
Lane 1: HEK-293T cells transfected with the pCMV6-ENTRY control at 5 µg
Lane 2: HEK-293T cells transfected with pCMV6-ENTRY MAK at 5 µg
Predicted band size: 71 kDa
![Western blot - Anti-MAK antibody [OTI1G2] (ab279383), expandable thumbnail](https://content.abcam.com/products/images/mak-antibody-oti1g2-ab279383--western-blot-img152461.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-MAK antibody [OTI1G2] (ab279383)")
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