Anti-Malondialdehyde antibody [11E3]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Malondialdehyde antibody [11E3] (AB243066)

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Malondialdehyde with ab243066 (green). Tissue was incubated with ab243066 at 1/100 overnight at 4^oC then for 30 minutes at 37^oC, followed by incubation with a Goat anti-Mouse IgG (H+L) : FITC secondary antibody for 45 minutes at 37^oC. Counterstain used was DAPI for 3 minutes at RT. Magnification : 5X.

• WB

Supplier Data

Western blot - Anti-Malondialdehyde antibody [11E3] (AB243066)

Primary incubation for 2 hours at room temperature.

Secondary incubation for 1 hour at room temperature.

Block : 5% Skimmed milk in TBST.

All lanes:

Western blot - Anti-Malondialdehyde antibody [11E3] (ab243066) at 1/1000 dilution

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate at 12 µg

Lane 2:

HeLa (treated with hydrogen peroxide) cell lysate at 12 µg

Secondary

All lanes:

Goat Anti-Mouse IgG: HRP at 1/2000 dilution

true

Exposure time: 5min

• WB

Supplier Data

Western blot - Anti-Malondialdehyde antibody [11E3] (AB243066)

Primary incubation for 2 hrs at RT.

Secondary incubation for 1 hr at RT.

Block : 5% Skimmed milk in TBST.

All lanes:

Western blot - Anti-Malondialdehyde antibody [11E3] (ab243066) at 1/1000 dilution

Lane 1:

Malondialdehyde-BSA at 0.5 µg

Lane 2:

Malondialdehyde-BSA at 2 µg

Lane 3:

BSA at 0.5 µg

Lane 4:

BSA at 2 µg

Secondary

All lanes:

Goat Anti-Mouse IgG: HRP at 1/2000 dilution

true

Exposure time: 5min