Rabbit Recombinant Monoclonal MALT1/MLT antibody. Carrier free. Suitable for IP, WB and reacts with Human, Mouse samples.
Images
![Immunoprecipitation - Anti-MALT1/MLT antibody [EPR24445-129] - BSA and Azide free (AB283590), expandable thumbnail](https://content.abcam.com/products/images/malt1-mlt-antibody-epr24445-129-bsa-and-azide-free-ab283590--immunoprecipitation-img173936.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-MALT1/MLT antibody [EPR24445-129] - BSA and Azide free (AB283590)")
![Western blot - Anti-MALT1/MLT antibody [EPR24445-129] - BSA and Azide free (AB283590), expandable thumbnail](https://content.abcam.com/products/images/malt1-mlt-antibody-epr24445-129-bsa-and-azide-free-ab283590--western-blot-img164306.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-MALT1/MLT antibody [EPR24445-129] - BSA and Azide free (AB283590)")
![Western blot - Anti-MALT1/MLT antibody [EPR24445-129] - BSA and Azide free (AB283590), expandable thumbnail](https://content.abcam.com/products/images/malt1-mlt-antibody-epr24445-129-bsa-and-azide-free-ab283590--western-blot-img172418.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-MALT1/MLT antibody [EPR24445-129] - BSA and Azide free (AB283590)")
![Western blot - Anti-MALT1/MLT antibody [EPR24445-129] - BSA and Azide free (AB283590), expandable thumbnail](https://content.abcam.com/products/images/malt1-mlt-antibody-epr24445-129-bsa-and-azide-free-ab283590--western-blot-img441691.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-MALT1/MLT antibody [EPR24445-129] - BSA and Azide free (AB283590)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | IHC-P | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Not recommended | Not recommended | Not recommended |
| Mouse | Not recommended | Tested | Not recommended | Not recommended | Not recommended |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
Target data
Function
Protease that enhances BCL10-induced activation: acts via formation of CBM complexes that channel adaptive and innate immune signaling downstream of CARD domain-containing proteins (CARD9, CARD11 and CARD14) to activate NF-kappa-B and MAP kinase p38 pathways which stimulate expression of genes encoding pro-inflammatory cytokines and chemokines (PubMed:11262391, PubMed:18264101, PubMed:24074955). Mediates BCL10 cleavage: MALT1-dependent BCL10 cleavage plays an important role in T-cell antigen receptor-induced integrin adhesion (PubMed:11262391, PubMed:18264101). Involved in the induction of T helper 17 cells (Th17) differentiation (PubMed:11262391, PubMed:18264101). Cleaves RC3H1 and ZC3H12A in response to T-cell receptor (TCR) stimulation which releases their cooperatively repressed targets to promote Th17 cell differentiation (By similarity). Also mediates cleavage of N4BP1 in T-cells following TCR-mediated activation, leading to N4BP1 inactivation (PubMed:31133753). May also have ubiquitin ligase activity: binds to TRAF6, inducing TRAF6 oligomerization and activation of its ligase activity (PubMed:14695475).
Alternative names
MLT, MALT1, Mucosa-associated lymphoid tissue lymphoma translocation protein 1, MALT lymphoma-associated translocation, Paracaspase
Recommended products
Rabbit Recombinant Monoclonal MALT1/MLT antibody. Carrier free. Suitable for IP, WB and reacts with Human, Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR24445-129
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab283590 is the carrier-free version of Anti-MALT1/MLT antibody [EPR24445-129] ab283573.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Immunoprecipitation - Anti-MALT1/MLT antibody [EPR24445-129] - BSA and Azide free (ab283590), expandable thumbnail](https://content.abcam.com/products/images/malt1-mlt-antibody-epr24445-129-bsa-and-azide-free-ab283590--immunoprecipitation-img173936.jpg "Immunoprecipitation - Anti-MALT1/MLT antibody [EPR24445-129] - BSA and Azide free (ab283590)")
Immunoprecipitation - Anti-MALT1/MLT antibody [EPR24445-129] - BSA and Azide free (ab283590)
This data was developed using Anti-MALT1/MLT antibody [EPR24445-129] ab283573, the same antibody clone in a different buffer formulation.
MALT1/MLT was immunoprecipitated from 0.35 mg Ramos (human Burkitt's lymphoma B lymphocyte), whole cell lysate 10 ug with Anti-MALT1/MLT antibody [EPR24445-129] ab283573 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-MALT1/MLT antibody [EPR24445-129] ab283573 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: Ramos (human Burkitt's lymphoma B lymphocyte), whole cell lysate 10 ug
Lane 2: Anti-MALT1/MLT antibody [EPR24445-129] ab283573 IP in Ramos whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-MALT1/MLT antibody [EPR24445-129] ab283573 in Ramos whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes
All lanes: Immunoprecipitation - Anti-MALT1/MLT antibody [EPR24445-129] (Anti-MALT1/MLT antibody [EPR24445-129] ab283573)
Predicted band size: 92 kDa
Observed band size: 92 kDa
![Western blot - Anti-MALT1/MLT antibody [EPR24445-129] - BSA and Azide free (ab283590), expandable thumbnail](https://content.abcam.com/products/images/malt1-mlt-antibody-epr24445-129-bsa-and-azide-free-ab283590--western-blot-img164306.jpg "Western blot - Anti-MALT1/MLT antibody [EPR24445-129] - BSA and Azide free (ab283590)")
Western blot - Anti-MALT1/MLT antibody [EPR24445-129] - BSA and Azide free (ab283590)
This data was developed using Anti-MALT1/MLT antibody [EPR24445-129] ab283573, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Anti-MALT1/MLT antibody [EPR24445-129] ab283573 Anti-MALT1/MLT antibody [EPR24445-129] was shown to specifically react with MALT1/MLT in wild-type HeLa cells. Loss of signal was observed when knockout cell line Human MALT1 (MLT) knockout HeLa cell line ab264930 (knockout cell lysate Human MALT1 (MLT) knockout HeLa cell lysate ab257149) was used. Wild-type and MALT1/MLT knockout samples were subjected to SDS-PAGE.
Exposure time: 37 seconds
All lanes: Western blot - Anti-MALT1/MLT antibody [EPR24445-129] (Anti-MALT1/MLT antibody [EPR24445-129] ab283573) at 1/1000 dilution
Lane 1: Wild-type HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2: MALT1/MLT knockout Hela whole cell lysate at 20 µg
Lane 3: Ramos (human Burkitt's lymphoma B lymphocyte) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 92 kDa
Observed band size: 92 kDa
![Western blot - Anti-MALT1/MLT antibody [EPR24445-129] - BSA and Azide free (ab283590), expandable thumbnail](https://content.abcam.com/products/images/malt1-mlt-antibody-epr24445-129-bsa-and-azide-free-ab283590--western-blot-img172418.jpg "Western blot - Anti-MALT1/MLT antibody [EPR24445-129] - BSA and Azide free (ab283590)")
Western blot - Anti-MALT1/MLT antibody [EPR24445-129] - BSA and Azide free (ab283590)
This data was developed using Anti-MALT1/MLT antibody [EPR24445-129] ab283573, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: Lane 1: 26 seconds ; Lane 2: 70 seconds
All lanes: Western blot - Anti-MALT1/MLT antibody [EPR24445-129] (Anti-MALT1/MLT antibody [EPR24445-129] ab283573) at 1/1000 dilution
Lane 1: Mouse thymus tissue lysate at 20 µg
Lane 2: A20 (mouse reticum sarcoma B lymphocyte) whole cell lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 92 kDa
Observed band size: 92 kDa
![Western blot - Anti-MALT1/MLT antibody [EPR24445-129] - BSA and Azide free (ab283590), expandable thumbnail](https://content.abcam.com/products/images/malt1-mlt-antibody-epr24445-129-bsa-and-azide-free-ab283590--western-blot-img441691.jpg "Western blot - Anti-MALT1/MLT antibody [EPR24445-129] - BSA and Azide free (ab283590)")
Western blot - Anti-MALT1/MLT antibody [EPR24445-129] - BSA and Azide free (ab283590)
This data was developed using Anti-MALT1/MLT antibody [EPR24445-129] ab283573, the same antibody clone in a different buffer formulation.
Western blot: Rabbit Monoclonal[EPR24445-129] to MALT1/MLT Anti-MALT1/MLT antibody [EPR24445-129] ab283573 staining at 1/1000 dilution, shown in green; Mouse anti GAPDH (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20,000 dilution, shown in magenta. A band was observed at kDa in Wild-type HCT 116 ab288559 cell lysates with no signal observed at this size in MALT1 knockout HCT 116 Human MALT1 knockout HCT116 cell line ab286597 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution.
All lanes: Western blot - Anti-MALT1/MLT antibody [EPR24445-129] (Anti-MALT1/MLT antibody [EPR24445-129] ab283573) at 1/1000 dilution
Lane 1: Wild-type HCT 116 ab288559 at 20 µg
Lane 2: MALT1 knockout HCT 116 Human MALT1 knockout HCT116 cell line ab286597 at 20 µg
Lane 3: HeLa at 20 µg
Lane 4: Ramos at 20 µg
SecondaryAll lanes: Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution
Performed under reducing conditions.
Predicted band size: 92 kDa
Observed band size: 95 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com