Anti-MALT1/MLT antibody [EPR24445-129] - BSA and Azide free

• IP

Supplier Data

Immunoprecipitation - Anti-MALT1/MLT antibody [EPR24445-129] - BSA and Azide free (AB283590)

This data was developed using ab283573, the same antibody clone in a different buffer formulation.

MALT1/MLT was immunoprecipitated from 0.35 mg Ramos (human Burkitt's lymphoma B lymphocyte), whole cell lysate 10 ug with ab283573 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab283573 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : Ramos (human Burkitt's lymphoma B lymphocyte), whole cell lysate 10 ug

Lane 2 : ab283573 IP in Ramos whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab283573 in Ramos whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 minutes

All lanes:

Immunoprecipitation - Anti-MALT1/MLT antibody [EPR24445-129] (<a href='/en-us/products/primary-antibodies/malt1-mlt-antibody-epr24445-129-ab283573'>ab283573</a>)

Predicted band size: 92 kDa

Observed band size: 92 kDa

false

• WB

Lab

Western blot - Anti-MALT1/MLT antibody [EPR24445-129] - BSA and Azide free (AB283590)

This data was developed using ab283573, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

ab283573 Anti-MALT1/MLT antibody [EPR24445-129] was shown to specifically react with MALT1/MLT in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264930 (knockout cell lysate ab257149) was used. Wild-type and MALT1/MLT knockout samples were subjected to SDS-PAGE.

Exposure time : 37 seconds

All lanes:

Western blot - Anti-MALT1/MLT antibody [EPR24445-129] (<a href='/en-us/products/primary-antibodies/malt1-mlt-antibody-epr24445-129-ab283573'>ab283573</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

MALT1/MLT knockout Hela whole cell lysate at 20 µg

Lane 3:

Ramos (human Burkitt's lymphoma B lymphocyte) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 92 kDa

Observed band size: 92 kDa

false

• WB

Lab

Western blot - Anti-MALT1/MLT antibody [EPR24445-129] - BSA and Azide free (AB283590)

This data was developed using ab283573, the same antibody clone in a different buffer formulation.

Western blot : Rabbit Monoclonal[EPR24445-129] to MALT1/MLT ab283573 staining at 1/1000 dilution, shown in green; Mouse anti GAPDH (ab8245) loading control staining at 1/20,000 dilution, shown in magenta. A band was observed at kDa in Wild-type HCT 116 ab288559 cell lysates with no signal observed at this size in MALT1 knockout HCT 116 ab286597 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-MALT1/MLT antibody [EPR24445-129] (<a href='/en-us/products/primary-antibodies/malt1-mlt-antibody-epr24445-129-ab283573'>ab283573</a>) at 1/1000 dilution

Lane 1:

Wild-type HCT 116 ab288559 at 20 µg

Lane 2:

Western blot - Human MALT1 knockout HCT116 cell line (<a href='/en-us/products/cell-lines/human-malt1-knockout-hct116-cell-line-ab286597'>ab286597</a>) at 20 µg

Lane 3:

HeLa at 20 µg

Lane 4:

Ramos at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 92 kDa

Observed band size: 95 kDa

false

• WB

Lab

Western blot - Anti-MALT1/MLT antibody [EPR24445-129] - BSA and Azide free (AB283590)

This data was developed using ab283573, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Exposure time : Lane 1 : 26 seconds ; Lane 2 : 70 seconds

All lanes:

Western blot - Anti-MALT1/MLT antibody [EPR24445-129] (<a href='/en-us/products/primary-antibodies/malt1-mlt-antibody-epr24445-129-ab283573'>ab283573</a>) at 1/1000 dilution

Lane 1:

Mouse thymus tissue lysate at 20 µg

Lane 2:

A20 (mouse reticum sarcoma B lymphocyte) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 92 kDa

Observed band size: 92 kDa

false