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AB203583

Anti-Maltose Binding Protein antibody

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Rabbit Polyclonal Maltose Binding Protein antibody. Suitable for WB, ELISA and reacts with Tag, Escherichia coli samples. Immunogen corresponding to Synthetic Peptide within Escherichia coli K-12 malE aa 1-100 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

b4034, JW3994, malE, Maltose/maltodextrin-binding periplasmic protein, MMBP, Maltodextrin-binding protein, Maltose-binding protein, MBP

1 Images
ELISA - Anti-Maltose Binding Protein antibody (AB203583)
  • ELISA

Supplier Data

ELISA - Anti-Maltose Binding Protein antibody (AB203583)

Antigen : Maltose Binding Protein, 0.2ug/100ul Primary : ab203583, 1/500, 1/1000, 1/2000, 1/4000, 1/8000, 1/16000, 1/32000; Secondary : HRP conjugated Goat Anti-Rabbit IgG at 1/5000; TMB staining; Read the data in MicroplateReader by 450nm.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Escherichia coli

Applications

WB, ELISA

applications

Immunogen

Synthetic Peptide within Escherichia coli K-12 malE aa 1-100 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P0AEX9

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Escherichia coli": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>" }, "Tag": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/1000", "WB-species-notes": "<p></p>", "ELISA-species-checked": "notRecommended", "ELISA-species-dilution-info": "", "ELISA-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Maltose Binding Protein often referred to as MBP is a periplasmic protein that plays an important role in the transportation of maltose and maltodextrins in E. coli. It binds to maltose with high specificity facilitating its uptake into the bacterial cell. The MBP protein size is approximately 42 kDa which allows it to effectively interact with its ligands. Scientists often use MBP as a fusion partner in protein purification processes due to its solubility and ability to enhance the expression of recombinant proteins.
Biological function summary

Within the cell it functions by ensuring the effective transport of sugars across the bacterial membrane. Since MBP is not typically a part of a larger complex it acts more as an individual unit facilitating the binding of its target sugars. This binding and transport mechanism is vital for the bacterial carbohydrate metabolism supporting the overall energy balance within the microorganism.

Pathways

This protein is integral to the maltose/maltodextrin transport system which is a part of the larger phosphotransferase system. Its role in these pathways is closely associated with other proteins such as the Maltose ABC transporter proteins which form a transport mechanism across the bacterial inner membrane. Additionally MBP has a functional relationship with the enzymes in the glycolysis pathway as the transported sugars eventually feed into this energy-producing route.

Maltose binding protein itself is not a direct causative factor. However understanding MBP can assist in researching bacterial infections caused by pathogenic strains like E. coli where maltose metabolism is important for survival and virulence. Moreover disruptions in sugar transport pathways involving proteins like MBP can contribute to metabolic imbalances. Although MBP is not directly implicated in human diseases analyzing its function can aid in developing therapeutic approaches targeting sugar transport systems in harmful bacteria.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Part of the ABC transporter complex MalEFGK involved in maltose/maltodextrin import. Binds maltose and higher maltodextrins such as maltotriose.
See full target information malE

Additional targets

Maltose Binding Protein
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Product promise

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