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AB119994

Anti-Maltose Binding Protein antibody [EPR4744]

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(8 Publications)

Rabbit Recombinant Monoclonal Maltose Binding Protein antibody. Suitable for WB and reacts with Escherichia coli samples. Cited in 8 publications.

View Alternative Names

b4034, JW3994, malE, Maltose/maltodextrin-binding periplasmic protein, MMBP, Maltodextrin-binding protein, Maltose-binding protein, MBP

1 Images
Western blot - Anti-Maltose Binding Protein antibody [EPR4744] (AB119994)
  • WB

Unknown

Western blot - Anti-Maltose Binding Protein antibody [EPR4744] (AB119994)

All lanes:

Western blot - Anti-Maltose Binding Protein antibody [EPR4744] (ab119994) at 1/1000 dilution

All lanes:

E.coli lysate at 10 µg/mL

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 43 kDa

false

  • Carrier free

    Anti-Maltose Binding Protein antibody [EPR4744] - BSA and Azide free

  • HRP

    HRP Anti-Maltose Binding Protein antibody [EPR4744]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4744

Isotype

IgG

Carrier free

No

Reacts with

Escherichia coli

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Escherichia coli": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Maltose Binding Protein often referred to as MBP is a periplasmic protein that plays an important role in the transportation of maltose and maltodextrins in E. coli. It binds to maltose with high specificity facilitating its uptake into the bacterial cell. The MBP protein size is approximately 42 kDa which allows it to effectively interact with its ligands. Scientists often use MBP as a fusion partner in protein purification processes due to its solubility and ability to enhance the expression of recombinant proteins.
Biological function summary

Within the cell it functions by ensuring the effective transport of sugars across the bacterial membrane. Since MBP is not typically a part of a larger complex it acts more as an individual unit facilitating the binding of its target sugars. This binding and transport mechanism is vital for the bacterial carbohydrate metabolism supporting the overall energy balance within the microorganism.

Pathways

This protein is integral to the maltose/maltodextrin transport system which is a part of the larger phosphotransferase system. Its role in these pathways is closely associated with other proteins such as the Maltose ABC transporter proteins which form a transport mechanism across the bacterial inner membrane. Additionally MBP has a functional relationship with the enzymes in the glycolysis pathway as the transported sugars eventually feed into this energy-producing route.

Maltose binding protein itself is not a direct causative factor. However understanding MBP can assist in researching bacterial infections caused by pathogenic strains like E. coli where maltose metabolism is important for survival and virulence. Moreover disruptions in sugar transport pathways involving proteins like MBP can contribute to metabolic imbalances. Although MBP is not directly implicated in human diseases analyzing its function can aid in developing therapeutic approaches targeting sugar transport systems in harmful bacteria.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Part of the ABC transporter complex MalEFGK involved in maltose/maltodextrin import. Binds maltose and higher maltodextrins such as maltotriose.
See full target information malE
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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

EMBO reports 26:3411-3444 PubMed40410380

2025

Dynamic SAS-6 phosphorylation aids centrosome duplication and elimination in C. elegans oogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Feifei Qi,Shanshan Yin,Xiangrui Yang,Ning Ju,Bohan Liu,Xing Zhang,Zixuan Zhu,Li Ji,Fuxin Zhang,Li Zhao,Ruoxi Wang,Min Liu,Liangran Zhang,Huijie Zhao,Jun Zhou,Jinmin Gao

Communications biology 8:626 PubMed40247087

2025

The role of ladderlectin in spermatogenesis and ovarian sperm storage in the black rockfish (Sebastes schlegelii).

Applications

Unspecified application

Species

Unspecified reactive species

Ruiyan Yang,Na Wang,Weihao Song,Fengyan Zhang,Xiangyu Gao,Hao Sun,Tianci Nie,Gongchen Liu,Mengda Du,Fuxiang Liu,Hang Zhang,Jie Qi,Yan He

Nature communications 15:4634 PubMed38821984

2024

Evolved histone tail regulates 53BP1 recruitment at damaged chromatin.

Applications

Unspecified application

Species

Unspecified reactive species

Jessica L Kelliher,Melissa L Folkerts,Kaiyuan V Shen,Wan Song,Kyle Tengler,Clara M Stiefel,Seong-Ok Lee,Eloise Dray,Weixing Zhao,Brian Koss,Nicholas R Pannunzio,Justin W Leung

Nature communications 15:3789 PubMed38710693

2024

A conserved N-terminal motif of CUL3 contributes to assembly and E3 ligase activity of CRL3.

Applications

Unspecified application

Species

Unspecified reactive species

Weize Wang,Ling Liang,Zonglin Dai,Peng Zuo,Shang Yu,Yishuo Lu,Dian Ding,Hongyi Chen,Hui Shan,Yan Jin,Youdong Mao,Yuxin Yin

The EMBO journal 43:1089-1109 PubMed38360992

2024

Structural insights into the ubiquitylation strategy of the oligomeric CRL2 E3 ubiquitin ligase.

Applications

Unspecified application

Species

Unspecified reactive species

Zonglin Dai,Ling Liang,Weize Wang,Peng Zuo,Shang Yu,Yaqi Liu,Xuyang Zhao,Yishuo Lu,Yan Jin,Fangting Zhang,Dian Ding,Weiwei Deng,Yuxin Yin

The Journal of biological chemistry 299:105043 PubMed37451480

2023

UBA80 and UBA52 fine-tune RNF168-dependent histone ubiquitination and DNA repair.

Applications

Unspecified application

Species

Unspecified reactive species

Seong-Ok Lee,Jessica L Kelliher,Wan Song,Kyle Tengler,Aradhan Sarkar,Eloise Dray,Justin W C Leung

Nature communications 14:3916 PubMed37400439

2023

A PPP-type pseudophosphatase is required for the maintenance of basal complex integrity in Plasmodium falciparum.

Applications

Unspecified application

Species

Unspecified reactive species

Alexander A Morano,Rachel M Rudlaff,Jeffrey D Dvorin

Genes & development 31:2361-2375 PubMed29317486

2018

Mutual dependence of the MRTF-SRF and YAP-TEAD pathways in cancer-associated fibroblasts is indirect and mediated by cytoskeletal dynamics.

Applications

Unspecified application

Species

Unspecified reactive species

Charles T Foster,Francesco Gualdrini,Richard Treisman
View all publications
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