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AB65

Anti-Maltose Binding Protein antibody [R29.6]

5

(2 Reviews)

|

(9 Publications)

Mouse Monoclonal Maltose Binding Protein antibody. Suitable for WB, ChIP, IP and reacts with Escherichia coli samples. Cited in 9 publications.

View Alternative Names

b4034, JW3994, malE, Maltose/maltodextrin-binding periplasmic protein, MMBP, Maltodextrin-binding protein, Maltose-binding protein, MBP

1 Images
Western blot - Anti-Maltose Binding Protein antibody [R29.6] (AB65)
  • WB

PubMed

Western blot - Anti-Maltose Binding Protein antibody [R29.6] (AB65)

Representative western blot detecting Maltose Binding Protein using ab65 at 1.8 µg/ml.

All lanes:

Western blot - Anti-Maltose Binding Protein antibody [R29.6] (ab65)

Predicted band size: 43 kDa

false

Image from Verhoeven GS et al., PLoS One. 2009 Aug 25;4(8):e6739.Fig S6.; doi:10.1371/journal.pone.0006739; August 25, 2009, PLoS ONE 4(8): e6739.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

R29.6

Isotype

IgG1

Light chain type

unknown

Carrier free

No

Reacts with

Escherichia coli

Applications

IP, WB, ChIP

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Escherichia coli": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "<p></p>", "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "<p></p>", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>" } } }
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Product details

MBP is a bacterial protein, which is also used as a fusion protein in expression constructs.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.02% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Maltose Binding Protein often referred to as MBP is a periplasmic protein that plays an important role in the transportation of maltose and maltodextrins in E. coli. It binds to maltose with high specificity facilitating its uptake into the bacterial cell. The MBP protein size is approximately 42 kDa which allows it to effectively interact with its ligands. Scientists often use MBP as a fusion partner in protein purification processes due to its solubility and ability to enhance the expression of recombinant proteins.
Biological function summary

Within the cell it functions by ensuring the effective transport of sugars across the bacterial membrane. Since MBP is not typically a part of a larger complex it acts more as an individual unit facilitating the binding of its target sugars. This binding and transport mechanism is vital for the bacterial carbohydrate metabolism supporting the overall energy balance within the microorganism.

Pathways

This protein is integral to the maltose/maltodextrin transport system which is a part of the larger phosphotransferase system. Its role in these pathways is closely associated with other proteins such as the Maltose ABC transporter proteins which form a transport mechanism across the bacterial inner membrane. Additionally MBP has a functional relationship with the enzymes in the glycolysis pathway as the transported sugars eventually feed into this energy-producing route.

Maltose binding protein itself is not a direct causative factor. However understanding MBP can assist in researching bacterial infections caused by pathogenic strains like E. coli where maltose metabolism is important for survival and virulence. Moreover disruptions in sugar transport pathways involving proteins like MBP can contribute to metabolic imbalances. Although MBP is not directly implicated in human diseases analyzing its function can aid in developing therapeutic approaches targeting sugar transport systems in harmful bacteria.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Part of the ABC transporter complex MalEFGK involved in maltose/maltodextrin import. Binds maltose and higher maltodextrins such as maltotriose.
See full target information malE
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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 39:110633 PubMed35385720

2022

Diurnal rhythms in cholinergic modulation of rapid dopamine signals and associative learning in the striatum.

Applications

Unspecified application

Species

Unspecified reactive species

Taylor A Stowe,Elizabeth G Pitts,Amy C Leach,Melody C Iacino,Farr Niere,Benjamin Graul,Kimberly F Raab-Graham,Jordan T Yorgason,Mark J Ferris

Nature communications 12:5959 PubMed34645844

2021

Disulfide-compatible phage-assisted continuous evolution in the periplasmic space.

Applications

Unspecified application

Species

Unspecified reactive species

Mary S Morrison,Tina Wang,Aditya Raguram,Colin Hemez,David R Liu

Journal of integrative plant biology 63:646-661 PubMed32761943

2020

ABNORMAL SHOOT 6 interacts with KATANIN 1 and SHADE AVOIDANCE 4 to promote cortical microtubule severing and ordering in Arabidopsis.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanfeng Li,Meng Deng,Haofeng Liu,Yan Li,Yu Chen,Min Jia,Hui Xue,Jingxia Shao,Jun Zhao,Yafei Qi,Lijun An,Fei Yu,Xiayan Liu

Applied and environmental microbiology 77:291-301 PubMed21057026

2010

Characterization of cyanate metabolism in marine Synechococcus and Prochlorococcus spp.

Applications

Unspecified application

Species

Unspecified reactive species

Nina A Kamennaya,Anton F Post

PloS one 4:e6739 PubMed19707582

2009

Differential bacterial surface display of peptides by the transmembrane domain of OmpA.

Applications

WB

Species

Unspecified reactive species

Gertjan S Verhoeven,Svetlana Alexeeva,Marileen Dogterom,Tanneke den Blaauwen

Developmental cell 17:234-43 PubMed19686684

2009

Structure and function of the ESCRT-II-III interface in multivesicular body biogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Young Jun Im,Thomas Wollert,Evzen Boura,James H Hurley

Genome research 16:1517-28 PubMed17053089

2006

Whole-genome comparison of Leu3 binding in vitro and in vivo reveals the importance of nucleosome occupancy in target site selection.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao Liu,Cheol-Koo Lee,Joshua A Granek,Neil D Clarke,Jason D Lieb

Genome research 15:421-7 PubMed15710749

2005

DIP-chip: rapid and accurate determination of DNA-binding specificity.

Applications

ChIP

Species

Saccharomyces cerevisiae S288C

Xiao Liu,David M Noll,Jason D Lieb,Neil D Clarke

The Journal of biological chemistry 278:9042-51 PubMed12496278

2002

Apoptin induces tumor-specific apoptosis as a globular multimer.

Applications

ICC/IF

Species

Unspecified reactive species

Sirik R Leliveld,Ying-Hui Zhang,Jennifer L Rohn,Mathieu H M Noteborn,Jan Pieter Abrahams
View all publications
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