Anti-MAML2 antibody

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAML2 antibody (AB245612)

Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for MAML2 using ab245612 at 1/200 dilution in immunohistochemical analysis. DAB staining.

• IP

Supplier Data

Immunoprecipitation - Anti-MAML2 antibody (AB245612)

MAML2 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) using ab245612 at 3 μg/reaction. Western blot was performed on the immunoprecipitate using ab245612 at 1 μg/ml.

Lane 1 : ab245612 IP in HeLa whole cell lysate.
Lane 2 : Control IgG in HeLa whole cell lysate.

Detection : Chemiluminescence with an exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-MAML2 antibody (ab245612)

Predicted band size: 125 kDa

false

• WB

Supplier Data

Western blot - Anti-MAML2 antibody (AB245612)

All lanes:

Western blot - Anti-MAML2 antibody (ab245612) at 0.04 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg

Lane 3:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 5 µg

Lane 4:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 5:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg

Predicted band size: 125 kDa

true

Exposure time: 3min