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Rabbit Monoclonal Mammaglobin A antibody. C-terminal. Suitable for Flow Cyt (Intra), IHC-P and reacts with Human samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mammaglobin A antibody [SP319] - C-terminal (AB223504), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-Mammaglobin A antibody [SP319] - C-terminal (AB223504), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mammaglobin A antibody [SP319] - C-terminal (AB223504), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.6
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow Cyt (Intra)IHC-P
Human
Tested
Tested

Tested
Tested

Species

Human

Dilution info

1/100

Notes

Primary Antibody Incubation: 30 minutes at 4°C.

Tested
Tested

Species

Human

Dilution info

1/100

Notes

Antigen Retrieval: Boil tissue section in EDTA buffer, pH 8.0 for 10 minutes followed by cooling at room temperature for 20 minutes.

Primary Antibody Incubation: 10 minutes at room temperature.

Associated Products

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3 products for Alternative Product

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Alternative names

Recommended products

Rabbit Monoclonal Mammaglobin A antibody. C-terminal. Suitable for Flow Cyt (Intra), IHC-P and reacts with Human samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

SP319

Purification technique

Affinity purification Protein A/G

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mammaglobin A antibody [SP319] - C-terminal (ab223504), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mammaglobin A antibody [SP319] - C-terminal (ab223504)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human breast carcinoma tissue sections labeling Mammaglobin A with ab223504 at 1/100 dilution (1.08 μg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Cytoplasmic staining on the human breast carcinoma, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with ab223504 for 10 mins at room temperature.

  • Flow Cytometry (Intracellular) - Anti-Mammaglobin A antibody [SP319] - C-terminal (ab223504), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Mammaglobin A antibody [SP319] - C-terminal (ab223504)

    Flow cytometric analysis of MCF7 (human breast adenocarcinoma cell line) cells stained for Mammaglobin A using ab223504 (green) at 1/100 dilution compared to negative control of rabbit IgG (blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mammaglobin A antibody [SP319] - C-terminal (ab223504), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mammaglobin A antibody [SP319] - C-terminal (ab223504)

    Formalin-fixed, paraffin-embedded human breast ductal carcinoma tissue stained for Mammaglobin A with ab223504 at 1/100 dilution in immunohistochemical analysis.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com