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AB121854

Anti-MAN1 antibody

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(2 Publications)

Rabbit Polyclonal MAN1 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human LEMD3 aa 750 to C-terminus.

View Alternative Names

MAN1, LEMD3, Inner nuclear membrane protein Man1, LEM domain-containing protein 3

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAN1 antibody (AB121854)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAN1 antibody (AB121854)

ab121854, at a 1/50 dilution, staining MAN1 in human rectum shows strong nuclear and cytoplasmic positivity in glandular cells.

Western blot - Anti-MAN1 antibody (AB121854)
  • WB

Supplier Data

Western blot - Anti-MAN1 antibody (AB121854)

All lanes:

Western blot - Anti-MAN1 antibody (ab121854) at 0.4 µg/mL

Lane 1:

Daudi (human Burkitt's lymphoma cell line) whole cell lysate

Lane 2:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Predicted band size: 100 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human LEMD3 aa 750 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9Y2U8

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Can function as a specific repressor of TGF-beta, activin, and BMP signaling through its interaction with the R-SMAD proteins. Antagonizes TGF-beta-induced cell proliferation arrest.
See full target information LEMD3
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

International journal of genomics 2025:6587097 PubMed40438725

2025

COL22A1 Activates the PI3K/AKT Signaling Pathway to Sustain the Malignancy of Glioblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Zheng,Yuanzhi Huang,Dong Chu,Shiming He

Nature communications 11:2122 PubMed32358486

2020

Role of the nuclear membrane protein Emerin in front-rear polarity of the nucleus.

Applications

Unspecified application

Species

Unspecified reactive species

Paulina Nastały,Divya Purushothaman,Stefano Marchesi,Alessandro Poli,Tobias Lendenmann,Gururaj Rao Kidiyoor,Galina V Beznoussenko,Stefania Lavore,Orso Maria Romano,Dimos Poulikakos,Marco Cosentino Lagomarsino,Alexander A Mironov,Aldo Ferrari,Paolo Maiuri
View all publications
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Product promise

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