Rabbit Polyclonal MAN1A2 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human MAN1A2 aa 100-200.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human MAN1A2 aa 100-200. The exact immunogen used to generate this antibody is proprietary information. Database link O60476
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200.00000 - 1/500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes - |
Target data
Function
Involved in the maturation of Asn-linked oligosaccharides. Progressively trim alpha-1,2-linked mannose residues from Man(9)GlcNAc(2) to produce Man(5)GlcNAc(2).
Alternative names
MAN1B, MAN1A2, Mannosidase alpha class 1A member 2
Recommended products
Rabbit Polyclonal MAN1A2 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human MAN1A2 aa 100-200.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human MAN1A2 aa 100-200. The exact immunogen used to generate this antibody is proprietary information. Database link O60476
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
Western blot - Anti-MAN1A2 antibody (ab272611)
Lane 1: Western blot - Anti-MAN1A2 antibody (ab272611)
Lane 2: Western blot - Anti-MAN1A2 antibody (ab272611) at 0.4 µg/mL
Lane 1: Molecular weight ladder
Lane 2: Human cell line RT4
Predicted band size: 73 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAN1A2 antibody (ab272611)
Paraffin-embedded formalin-fixed Human skeletal tissue stained for MAN1A2 using ab272611 at 1/500 in immunohistochemical analysis. Low expression as expected.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAN1A2 antibody (ab272611)
Paraffin-embedded formalin-fixed Human kidney tissue stained for MAN1A2 using ab272611 at 1/500 in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAN1A2 antibody (ab272611)
Paraffin-embedded formalin-fixed Human prostate tissue stained for MAN1A2 using ab272611 at 1/500 in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence - Anti-MAN1A2 antibody (ab272611)
PFA fixed, Triton X-100 permeablized human cell line A-431 labeling MAN1A2 (green) using ab272611at 2 ug/ml in ICC/IF analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAN1A2 antibody (ab272611)
Paraffin-embedded formalin-fixed Human placenta tissue stained for MAN1A2 using ab272611 at 1/500 in immunohistochemical analysis.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com