Anti-MANBA antibody [EPR27977-48]

8 product images

• 

  Western blot - Anti-MANBA antibody [EPR27977-48] (ab317496)

  Low expression: skeletal muscle(PMID:9384606), NIH/3T3

  The identity of the bands lower than 100 kDa are unknown.

  In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

  All lanes: Western blot - Anti-MANBA antibody [EPR27977-48] (ab317496) at 1/1000 dilution

  Lane 1: Mouse pancreas tissue lysate at 20 µg with NFDM/TBST

  Lane 2: Mouse lung tissue lysate at 20 µg with NFDM/TBST

  Lane 3: Mouse liver tissue lysate at 20 µg with NFDM/TBST

  Lane 4: Mouse kidney tissue lysate at 20 µg with NFDM/TBST

  Lane 5: Mouse skeletal muscle tissue lysate at 20 µg with NFDM/TBST

  Lane 6: RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg with NFDM/TBST

  Lane 7: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg with NFDM/TBST

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

  Developed using the ECL technique.

  Observed band size: 101 kDa, 36 kDa

  Exposure time: 125s

• 

  Western blot - Anti-MANBA antibody [EPR27977-48] (ab317496)

  In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

  All lanes: Western blot - Anti-MANBA antibody [EPR27977-48] (ab317496) at 1/1000 dilution

  Lane 1: NMuLi (mouse liver epithelial) whole cell lysate at 20 µg with NFDM/TBST

  Lane 2: Beta-TC-6 (mouse pancreas insinoma beta cell) whole cell lysate µg at 20 µg with NFDM/TBST

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

  Developed using the ECL technique.

  Observed band size: 101 kDa, 36 kDa

  Exposure time: 125s

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MANBA antibody [EPR27977-48] (ab317496)

  Immunohistochemical analysis of paraffin-embedded Rat skeletal muscle tissue labeling MANBA with ab317496 at 1/800 (0.643 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Low expression tissue: No staining on rat skeletal muscle.
  
  The section was incubated with ab317496 for 30 mins at room temperature.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument
  
  Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background.

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MANBA antibody [EPR27977-48] (ab317496)

  Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissue labeling MANBA with ab317496 at 1/800 (0.643 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Low expression tissue: No staining on mouse skeletal muscle.
  
  The section was incubated with ab317496 for 30 mins at room temperature.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument
  
  Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background.

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MANBA antibody [EPR27977-48] (ab317496)

  Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling MANBA with ab317496 at 1/800 (0.643 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Positive staining on rat liver.
  
  The section was incubated with ab317496 for 30 mins at room temperature.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument
  
  Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background.

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MANBA antibody [EPR27977-48] (ab317496)

  Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling MANBA with ab317496 at 1/800 (0.643 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Positive staining on rat kidney.
  
  The section was incubated with ab317496 for 30 mins at room temperature.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument
  
  Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background.

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MANBA antibody [EPR27977-48] (ab317496)

  Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling MANBA with ab317496 at 1/800 (0.643 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Positive staining on mouse liver.
  
  The section was incubated with ab317496 for 30 mins at room temperature.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument
  
  Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background.

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MANBA antibody [EPR27977-48] (ab317496)

  Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling MANBA with ab317496 at 1/800 (0.643 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Positive staining on mouse kidney (PMID: 33441424).
  
  The section was incubated with ab317496 for 30 mins at room temperature.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument
  
  Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background.

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins