AB23458

Anti-Mannan Binding Lectin/MBL antibody [1E2]

Name: Anti-Mannan Binding Lectin/MBL antibody [1E2] (ab23458) | Abcam
Brand: Abcam Limited
SKU: AB23458
Price: 520 USD
Availability: InStock

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(4 Publications)

Mouse Monoclonal Mannan Binding Lectin/MBL antibody. Suitable for ELISA, WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Full Length Protein corresponding to Human MBL2.

View Alternative Names

COLEC1, MBL, MBL2, Mannose-binding protein C, MBP-C, Collectin-1, MBP1, Mannan-binding protein, Mannose-binding lectin

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

1E2

Isotype

IgG1

Light chain type

kappa

Carrier free

Reacts with

Human

Applications

ELISA, WB

applications

Immunogen

Full Length Protein corresponding to Human MBL2. The exact immunogen used to generate this antibody is proprietary information.

P11226

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Reactivity data

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Product details

ab23458 reacts with human MBL both in its poltmeric conforation and as a single subunit of moleculat mass 26 kDa. Can be applied for quantitative measurement of MBL in plasma or serum.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS, 2.9% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Mannan Binding Lectin often referred to as MBL is an important player in the innate immune system. It is known alternatively as mannose-binding lectin or mannan-binding protein. The mass of MBL is approximately 32 to 34 kilodaltons and it is primarily produced in the liver. MBL circulates in the bloodstream where it can recognize patterns on the surface of pathogens such as bacteria and viruses through its lectin-binding domain. This recognition leads to the activation of other components that play roles in immune response.

Biological function summary

Mannan Binding Lectin serves a significant role in immune defense by binding to carbohydrate structures on pathogens. It is part of a complex known as the lectin pathway of the complement system one of three pathways that activate the complement system to enhance pathogen removal. MBL can activate through a series of proteases leading to opsonization cell lysis and inflammation which are vital responses to infection.

Pathways

Mannan recognition by MBL initiates the lectin pathway an essential component of the complement system. This pathway converges with the classical and alternative pathways to enhance the immune response. MBL works closely with MBL-associated serine proteases (MASPs) particularly MASP-1 and MASP-2 which cleave complement proteins resulting in pathogen opsonization and membrane attack complex formation. These actions are tightly regulated and ensure effective defense against invaders.

Deficiencies or polymorphisms in MBL can lead to increased susceptibility to infections and developmental complications of the immune system. Individuals with low MBL levels may experience recurrent respiratory infections or other bacterial infections due to inadequate complement activation. Moreover variations in the MBL protein have connections with autoimmune diseases such as systemic lupus erythematosus where MBL levels might influence disease severity. In these conditions altered interactions with other immune proteins like C4 and C3 may contribute to pathological responses.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Calcium-dependent lectin, which acts as a pattern recognition receptor that initiates the lectin pathway of the complement system, a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system (PubMed : 14515269, PubMed : 22966085, PubMed : 7634089, PubMed : 9087411). Specifically recognizes and binds the mannose moiety of carbohydrates on the pathogen surface, activating the MASP1 serine protease and initiating the proteolytic cascade of the lectin complement pathway (PubMed : 22966085, PubMed : 2573758, PubMed : 6643429, PubMed : 8082295, PubMed : 9087411). Upon SARS coronavirus-2/SARS-CoV-2 infection, activates the complement lectin pathway which leads to the inhibition SARS-CoV-2 infection and a reduction of the induced inflammatory response (PubMed : 35102342). May bind DNA (PubMed : 15145932).

See full target information MBL2

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Stem cells international 2021:1530445 PubMed34691188

2021

Exosomes Derived from miR-146a-5p-Enriched Mesenchymal Stem Cells Protect the Cardiomyocytes and Myocardial Tissues in the Polymicrobial Sepsis through Regulating MYBL1.

Applications

Unspecified application

Species

Unspecified reactive species

Chun Liu,Jianhua Xue,Bo Xu,Aixian Zhang,Lili Qin,Jiajia Liu,Yang Yang

Clinical and experimental immunology 144:512-20 PubMed16734621

2006

Design of a complement mannose-binding lectin pathway-specific activation system applicable at low serum dilutions.

Applications

Unspecified application

Species

Unspecified reactive species

M Harboe,P Garred,M S Borgen,G L Stahl,A Roos,T E Mollnes

Immunology 109:360-4 PubMed12807481

2003

Immature dendritic cells possess a sugar-sensitive receptor for human mannan-binding lectin.

Applications

Unspecified application

Species

Unspecified reactive species

Ian Downing,Claus Koch,David C Kilpatrick

Transplantation 75:1204-13 PubMed12717204

2003

Complement activation in early protocol kidney graft biopsies after living-donor transplantation.

Applications

Unspecified application

Species

Unspecified reactive species

Ståle Sund,Torstein Hovig,Anna Varberg Reisaeter,Helge Scott,Øystein Bentdal,Tom Eirik Mollnes

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Mannan Binding Lectin/MBL antibody [1E2]

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise