AB23457

Anti-Mannan Binding Lectin/MBL antibody [3B6]

Name: Anti-Mannan Binding Lectin/MBL antibody [3B6] (ab23457) | Abcam
Brand: Abcam Limited
SKU: AB23457
Price: 430 USD
Availability: InStock
Rating: 4 (2 reviews)

(2 Reviews)

(23 Publications)

Mouse Monoclonal Mannan Binding Lectin/MBL antibody. Suitable for IHC-P and reacts with Human samples. Cited in 23 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human MBL2.

View Alternative Names

COLEC1, MBL, MBL2, Mannose-binding protein C, MBP-C, Collectin-1, MBP1, Mannan-binding protein, Mannose-binding lectin

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannan Binding Lectin/MBL antibody [3B6] (AB23457)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannan Binding Lectin/MBL antibody [3B6] (AB23457)

ab23457 staining MBL in Paraffin-embedded Human Liver tissue by Immunohistochemistry.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannan Binding Lectin/MBL antibody [3B6] (AB23457)

ab23457 at 1 mg/ml staining human liver by IHC-P

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannan Binding Lectin/MBL antibody [3B6] (AB23457)

ab23457 staining MBL in Paraffin-embedded Human cancerous liver tissue by Immunohistochemistry.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

3B6

Isotype

IgG1

Light chain type

kappa

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Native Full Length Protein corresponding to Human MBL2.

P11226

Epitope

The epitope is on the head-neck region of the MBL protein chain.

Specificity

ab23457 is specific for MBL from human serum or plasma. In Western blotting, ab121075 reacts with Human MBL in both its oligomerized state.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250", "IHCP-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS, 2.9% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Mannan Binding Lectin often referred to as MBL is an important player in the innate immune system. It is known alternatively as mannose-binding lectin or mannan-binding protein. The mass of MBL is approximately 32 to 34 kilodaltons and it is primarily produced in the liver. MBL circulates in the bloodstream where it can recognize patterns on the surface of pathogens such as bacteria and viruses through its lectin-binding domain. This recognition leads to the activation of other components that play roles in immune response.

Biological function summary

Mannan Binding Lectin serves a significant role in immune defense by binding to carbohydrate structures on pathogens. It is part of a complex known as the lectin pathway of the complement system one of three pathways that activate the complement system to enhance pathogen removal. MBL can activate through a series of proteases leading to opsonization cell lysis and inflammation which are vital responses to infection.

Pathways

Mannan recognition by MBL initiates the lectin pathway an essential component of the complement system. This pathway converges with the classical and alternative pathways to enhance the immune response. MBL works closely with MBL-associated serine proteases (MASPs) particularly MASP-1 and MASP-2 which cleave complement proteins resulting in pathogen opsonization and membrane attack complex formation. These actions are tightly regulated and ensure effective defense against invaders.

Deficiencies or polymorphisms in MBL can lead to increased susceptibility to infections and developmental complications of the immune system. Individuals with low MBL levels may experience recurrent respiratory infections or other bacterial infections due to inadequate complement activation. Moreover variations in the MBL protein have connections with autoimmune diseases such as systemic lupus erythematosus where MBL levels might influence disease severity. In these conditions altered interactions with other immune proteins like C4 and C3 may contribute to pathological responses.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Calcium-dependent lectin, which acts as a pattern recognition receptor that initiates the lectin pathway of the complement system, a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system (PubMed : 14515269, PubMed : 22966085, PubMed : 7634089, PubMed : 9087411). Specifically recognizes and binds the mannose moiety of carbohydrates on the pathogen surface, activating the MASP1 serine protease and initiating the proteolytic cascade of the lectin complement pathway (PubMed : 22966085, PubMed : 2573758, PubMed : 6643429, PubMed : 8082295, PubMed : 9087411). Upon SARS coronavirus-2/SARS-CoV-2 infection, activates the complement lectin pathway which leads to the inhibition SARS-CoV-2 infection and a reduction of the induced inflammatory response (PubMed : 35102342). May bind DNA (PubMed : 15145932).

See full target information MBL2

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Publications (23)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:495 PubMed39753879

2025

Complement classical and alternative pathway activation contributes to diabetic kidney disease progression: a glomerular proteomics on kidney biopsies.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Yang,Ying Zhang,Yuan Li,Xinjin Zhou,Kazuho Honda,Dedong Kang,Muxi Wang,Jing-Hua Yang,Zongping Xia,Yuan Wei,Lu Liu,Ruimin Hu,Takashi Takaki,Guolan Xing

BMC nephrology 25:387 PubMed39478440

2024

The prognostic role of activation of the complement pathways in the progression of advanced IgA nephropathy to end-stage renal disease.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Wang,Shimin Jiang,Dingxin Di,Guming Zou,Hongmei Gao,Shunlai Shang,Wenge Li

International journal of general medicine 16:1879-1889 PubMed37213477

2023

Lectin Complement Pathway Activation is Associated with Massive Proteinuria in PLA2R-Positive Membranous Nephropathy: A Retrospective Study.

Applications

Unspecified application

Species

Unspecified reactive species

Jiayi Li,Jiao Zhang,Xu Wang,Xumin Zheng,Hongmei Gao,Shimin Jiang,Wenge Li

Translational research : the journal of laboratory and clinical medicine 259:35-45 PubMed37085047

2023

Diabetes mellitus and aortic stenosis head to head: toward personalized medicine in patients with both pathologies.

Applications

Unspecified application

Species

Unspecified reactive species

Nerea Corbacho-Alonso,Tamara Sastre-Oliva,Luis F López-Almodovar,Jorge Solis,Luis R Padial,Teresa Tejerina,Montserrat Carrascal,Laura Mourino-Alvarez,Maria G Barderas

Journal of inflammation research 15:4315-4329 PubMed35923908

2022

MBL Binding with AhR Controls Th17 Immunity in Silicosis-Associated Lung Inflammation and Fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yunzhi Liu,Na Zhao,Qishan Xu,Fan Deng,Ping Wang,Lijun Dong,Xiao Lu,Lihua Xia,Mingyong Wang,Zhengliang Chen,Jia Zhou,Daming Zuo

Frontiers in medicine 9:845679 PubMed35479942

2022

Activation of Complement Pathways in Kidney Tissue May Mediate Tubulointerstitial Injury in Diabetic Nephropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Shimin Jiang,Yuanyuan Jiao,Guming Zou,Hongmei Gao,Li Zhuo,Wenge Li

Cellular and molecular gastroenterology and hepatology 14:75-99 PubMed35381393

2022

Mannan-Binding Lectin via Interaction With Cell Surface Calreticulin Promotes Senescence of Activated Hepatic Stellate Cells to Limit Liver Fibrosis Progression.

Applications

Unspecified application

Species

Unspecified reactive species

Jialiang Luo,Lei Li,Bo Chang,Zhengyumeng Zhu,Fan Deng,Mengyao Hu,Yu Yu,Xiao Lu,Zhengliang Chen,Daming Zuo,Jia Zhou

Thoracic cancer 13:811-823 PubMed35137541

2022

Recruitment of IL-1β-producing intermediate monocytes enhanced by C5a contributes to the development of malignant pleural effusion.

Applications

Unspecified application

Species

Unspecified reactive species

Lisha Luo,Shuanglinzi Deng,Wei Tang,Xinyue Hu,Feifei Yin,Huan Ge,Jiale Tang,Zhonghua Liao,Xiaozhao Li,Juntao Feng

Journal of diabetes investigation 13:839-849 PubMed34932275

2022

Activation of complement C1q and C3 in glomeruli might accelerate the progression of diabetic nephropathy: Evidence from transcriptomic data and renal histopathology.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanyuan Jiao,Shimin Jiang,Ying Wang,Tianyu Yu,Guming Zou,Li Zhuo,Wenge Li

The Journal of clinical investigation 131: PubMed33351779

2020

Altered glycosylation of IgG4 promotes lectin complement pathway activation in anti-PLA2R1-associated membranous nephropathy.

Applications

Unspecified application

Species

Unspecified reactive species

George Haddad,Johan M Lorenzen,Hong Ma,Noortje de Haan,Harald Seeger,Christelle Zaghrini,Simone Brandt,Malte Kölling,Urs Wegmann,Bence Kiss,Gábor Pál,Péter Gál,Rudolf P Wüthrich,Manfred Wuhrer,Laurence H Beck,David J Salant,Gérard Lambeau,Andreas D Kistler

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Mannan Binding Lectin/MBL antibody [3B6]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannan Binding Lectin/MBL antibody [3B6] (AB23457)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannan Binding Lectin/MBL antibody [3B6] (AB23457)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannan Binding Lectin/MBL antibody [3B6] (AB23457)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Epitope

Specificity

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (23)

Product promise