AB189856

Anti-Mannan Binding Lectin/MBL antibody

Name: Anti-Mannan Binding Lectin/MBL antibody (ab189856) | Abcam
Brand: Abcam Limited
SKU: AB189856
Price: 535 USD
Availability: InStock

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(5 Publications)

Rabbit Polyclonal Mannan Binding Lectin/MBL antibody. Suitable for WB and reacts with Human, Mouse samples. Cited in 5 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human MBL2.

View Alternative Names

COLEC1, MBL, MBL2, Mannose-binding protein C, MBP-C, Collectin-1, MBP1, Mannan-binding protein, Mannose-binding lectin

1 Images

Supplier Data

Western blot - Anti-Mannan Binding Lectin/MBL antibody (AB189856)

All lanes:

Western blot - Anti-Mannan Binding Lectin/MBL antibody (ab189856) at 1/500 dilution

Lane 1:

HepG2 cell extract

Lane 2:

Mouse liver extract

Lane 3:

Mouse pancreas extract

Predicted band size: 26 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

applications

Immunogen

Recombinant Full Length Protein corresponding to Human MBL2.

P11226

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cynomolgus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Mannan Binding Lectin often referred to as MBL is an important player in the innate immune system. It is known alternatively as mannose-binding lectin or mannan-binding protein. The mass of MBL is approximately 32 to 34 kilodaltons and it is primarily produced in the liver. MBL circulates in the bloodstream where it can recognize patterns on the surface of pathogens such as bacteria and viruses through its lectin-binding domain. This recognition leads to the activation of other components that play roles in immune response.

Biological function summary

Mannan Binding Lectin serves a significant role in immune defense by binding to carbohydrate structures on pathogens. It is part of a complex known as the lectin pathway of the complement system one of three pathways that activate the complement system to enhance pathogen removal. MBL can activate through a series of proteases leading to opsonization cell lysis and inflammation which are vital responses to infection.

Pathways

Mannan recognition by MBL initiates the lectin pathway an essential component of the complement system. This pathway converges with the classical and alternative pathways to enhance the immune response. MBL works closely with MBL-associated serine proteases (MASPs) particularly MASP-1 and MASP-2 which cleave complement proteins resulting in pathogen opsonization and membrane attack complex formation. These actions are tightly regulated and ensure effective defense against invaders.

Deficiencies or polymorphisms in MBL can lead to increased susceptibility to infections and developmental complications of the immune system. Individuals with low MBL levels may experience recurrent respiratory infections or other bacterial infections due to inadequate complement activation. Moreover variations in the MBL protein have connections with autoimmune diseases such as systemic lupus erythematosus where MBL levels might influence disease severity. In these conditions altered interactions with other immune proteins like C4 and C3 may contribute to pathological responses.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Calcium-dependent lectin involved in innate immune defense (PubMed : 35102342). Binds mannose, fucose and N-acetylglucosamine on different microorganisms and activates the lectin complement pathway. Binds to late apoptotic cells, as well as to apoptotic blebs and to necrotic cells, but not to early apoptotic cells, facilitating their uptake by macrophages. May bind DNA. Upon SARS coronavirus-2/SARS-CoV-2 infection, activates the complement lectin pathway which leads to the inhibition SARS-CoV-2 infection and a reduction of the induced inflammatory response (PubMed : 35102342).

See full target information MBL2

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cellular and infection microbiology 15:1445660 PubMed40171164

2025

Divergent host humoral innate immune response to the smooth-to-rough adaptation of in chronic infection.

Applications

Unspecified application

Species

Unspecified reactive species

Emily A Wheeler,Patricia M Lenhart-Pendergrass,Noel M Rysavy,Katie R Poch,Silvia M Caceres,Kara M Calhoun,Karina A Serban,Jerry A Nick,Kenneth C Malcolm

Frontiers in immunology 13:948190 PubMed36032172

2022

Complement lectin pathway components MBL and MASP-1 promote haemostasis upon vessel injury in a microvascular bleeding model.

Applications

Unspecified application

Species

Unspecified reactive species

Murielle Golomingi,Jessie Kohler,Lorenz Jenny,Elaissa T Hardy,József Dobó,Péter Gál,Gábor Pál,Bence Kiss,Wilbur A Lam,Verena Schroeder

Clinical proteomics 17:20 PubMed32518534

2020

Proteomic analysis of human synovial fluid reveals potential diagnostic biomarkers for ankylosing spondylitis.

Applications

Unspecified application

Species

Unspecified reactive species

Ji-Hyun Lee,Jae Hun Jung,Jeesoo Kim,Won-Ki Baek,Jinseol Rhee,Tae-Hwan Kim,Sang-Hyon Kim,Kwang Pyo Kim,Chang-Nam Son,Jong-Seo Kim

Journal of clinical microbiology 58: PubMed32051260

2020

Direct Blood Culturing of spp. on Solid Medium by a Rapid Enrichment Method with Magnetic Beads Coated with Recombinant Human Mannan-Binding Lectin.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Li Chen,Hao Zheng,Wen-Ge Li,You-Hong Zhong,Xiao-Ping Chen,Jin-Xing Lu

Molecular medicine reports 17:2651-2659 PubMed29207142

2017

Screening and identification of differentially expressed serum proteins in patients with vitiligo using two‑dimensional gel electrophoresis coupled with mass spectrometry.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Lei Li,Rui-Qun Qi,Yang Yang,He-Xiao Wang,Hang-Hang Jiang,Zheng-Xiu Li,Bi-Huan Xiao,Song Zheng,Yu-Xiao Hong,Jiu-Hong Li,Hong-Duo Chen,Xing-Hua Gao

View all publications

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Product promise

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Anti-Mannan Binding Lectin/MBL antibody

Western blot - Anti-Mannan Binding Lectin/MBL antibody (AB189856)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

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