Rabbit Polyclonal Mannan Binding Lectin/MBL antibody. Suitable for IHC-P and reacts with Rat, Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human MBL2 aa 1-100 conjugated to Keyhole Limpet Haemocyanin.
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- Experimental neurology 326:1132032020Overexpression of Mfsd2a attenuates blood brain barrier dysfunction via Cav-1/Keap-1/Nrf-2/HO-1 pathway in a rat model of surgical brain injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPinar Eser Ocak et. al.PubMed 31954682
- Molecular medicine reports 17:2402-24142017Molecular basis of coronary artery dilation and aneurysms in patients with Kawasaki disease based on differential protein expression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWanting Liu,Chaowu Liu,Li Zhang,Xiaofei Xie,Xiaoqiong Gu,Chuanlan Sang,Mingguo Xu,Weijun Xu,Hongling JiaPubMed 29207079
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human MBL2 aa 1-100 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P11226
Reactivity data
Select an application| IHC-P | |
|---|---|
| Human | Tested |
| Rat | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/100.00000 - 1/500.00000 | Notes When using a fluorescent probe the recommended dilution is 1/50 – 1/200. |
Species Human | Dilution info 1/100.00000 - 1/500.00000 | Notes When using a fluorescent probe the recommended dilution is 1/50 – 1/200. |
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Target data
Function
Calcium-dependent lectin involved in innate immune defense (PubMed:35102342). Binds mannose, fucose and N-acetylglucosamine on different microorganisms and activates the lectin complement pathway. Binds to late apoptotic cells, as well as to apoptotic blebs and to necrotic cells, but not to early apoptotic cells, facilitating their uptake by macrophages. May bind DNA. Upon SARS coronavirus-2/SARS-CoV-2 infection, activates the complement lectin pathway which leads to the inhibition SARS-CoV-2 infection and a reduction of the induced inflammatory response (PubMed:35102342).
Alternative names
COLEC1, MBL, MBL2, Mannose-binding protein C, MBP-C, Collectin-1, MBP1, Mannan-binding protein, Mannose-binding lectin
Recommended products
Rabbit Polyclonal Mannan Binding Lectin/MBL antibody. Suitable for IHC-P and reacts with Rat, Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human MBL2 aa 1-100 conjugated to Keyhole Limpet Haemocyanin.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human MBL2 aa 1-100 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P11226
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Mannan Binding Lectin often referred to as MBL is an important player in the innate immune system. It is known alternatively as mannose-binding lectin or mannan-binding protein. The mass of MBL is approximately 32 to 34 kilodaltons and it is primarily produced in the liver. MBL circulates in the bloodstream where it can recognize patterns on the surface of pathogens such as bacteria and viruses through its lectin-binding domain. This recognition leads to the activation of other components that play roles in immune response.
- Biological function summary
Mannan Binding Lectin serves a significant role in immune defense by binding to carbohydrate structures on pathogens. It is part of a complex known as the lectin pathway of the complement system one of three pathways that activate the complement system to enhance pathogen removal. MBL can activate through a series of proteases leading to opsonization cell lysis and inflammation which are vital responses to infection.
- Pathways
Mannan recognition by MBL initiates the lectin pathway an essential component of the complement system. This pathway converges with the classical and alternative pathways to enhance the immune response. MBL works closely with MBL-associated serine proteases (MASPs) particularly MASP-1 and MASP-2 which cleave complement proteins resulting in pathogen opsonization and membrane attack complex formation. These actions are tightly regulated and ensure effective defense against invaders.
- Associated diseases and disorders
Deficiencies or polymorphisms in MBL can lead to increased susceptibility to infections and developmental complications of the immune system. Individuals with low MBL levels may experience recurrent respiratory infections or other bacterial infections due to inadequate complement activation. Moreover variations in the MBL protein have connections with autoimmune diseases such as systemic lupus erythematosus where MBL levels might influence disease severity. In these conditions altered interactions with other immune proteins like C4 and C3 may contribute to pathological responses.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannan Binding Lectin/MBL antibody (ab203303)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human esophageal carcinoma tissue labeling Mannan Binding Lectin/MBL with ab203303 at 1/200 dilution followed by conjugation to the secondary antibody and DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannan Binding Lectin/MBL antibody (ab203303)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Rat intestine tissue labeling Mannan Binding Lectin/MBL with ab203303 at 1/200 dilution followed by conjugation to the secondary antibody and DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannan Binding Lectin/MBL antibody (ab203303)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Rat liver tissue labeling Mannan Binding Lectin/MBL with ab203303 at 1/200 dilution followed by conjugation to the secondary antibody and DAB staining.
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