JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB288744

Anti-Mannan Binding Lectin/MBL antibody [YCA-R22129-183 H2L1] - BSA and Azide free (Capture)

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal Mannan Binding Lectin/MBL antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

View Alternative Names

COLEC1, MBL, MBL2, Mannose-binding protein C, MBP-C, Collectin-1, MBP1, Mannan-binding protein, Mannose-binding lectin

1 Images
Sandwich ELISA - Anti-Mannan Binding Lectin/MBL antibody [YCA-R22129-183 H2L1] - BSA and Azide free (Capture) (AB288744)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-Mannan Binding Lectin/MBL antibody [YCA-R22129-183 H2L1] - BSA and Azide free (Capture) (AB288744)

Example of Human MBL2 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

YCA-R22129-183 H2L1

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
style
left-column

Product details

ab288744 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human MLB2. The recommended pair for sandwich ELISA is:
Capture: ab288744, Human MLB2 Capture Antibody (unconjugated)
Detector: ab288747, Human MLB2 Detector Antibody (unconjugated)
The reference range value is 32 - 2000 pg/mL

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: 100% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Mannan Binding Lectin often referred to as MBL is an important player in the innate immune system. It is known alternatively as mannose-binding lectin or mannan-binding protein. The mass of MBL is approximately 32 to 34 kilodaltons and it is primarily produced in the liver. MBL circulates in the bloodstream where it can recognize patterns on the surface of pathogens such as bacteria and viruses through its lectin-binding domain. This recognition leads to the activation of other components that play roles in immune response.
Biological function summary

Mannan Binding Lectin serves a significant role in immune defense by binding to carbohydrate structures on pathogens. It is part of a complex known as the lectin pathway of the complement system one of three pathways that activate the complement system to enhance pathogen removal. MBL can activate through a series of proteases leading to opsonization cell lysis and inflammation which are vital responses to infection.

Pathways

Mannan recognition by MBL initiates the lectin pathway an essential component of the complement system. This pathway converges with the classical and alternative pathways to enhance the immune response. MBL works closely with MBL-associated serine proteases (MASPs) particularly MASP-1 and MASP-2 which cleave complement proteins resulting in pathogen opsonization and membrane attack complex formation. These actions are tightly regulated and ensure effective defense against invaders.

Deficiencies or polymorphisms in MBL can lead to increased susceptibility to infections and developmental complications of the immune system. Individuals with low MBL levels may experience recurrent respiratory infections or other bacterial infections due to inadequate complement activation. Moreover variations in the MBL protein have connections with autoimmune diseases such as systemic lupus erythematosus where MBL levels might influence disease severity. In these conditions altered interactions with other immune proteins like C4 and C3 may contribute to pathological responses.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Calcium-dependent lectin involved in innate immune defense (PubMed : 35102342). Binds mannose, fucose and N-acetylglucosamine on different microorganisms and activates the lectin complement pathway. Binds to late apoptotic cells, as well as to apoptotic blebs and to necrotic cells, but not to early apoptotic cells, facilitating their uptake by macrophages. May bind DNA. Upon SARS coronavirus-2/SARS-CoV-2 infection, activates the complement lectin pathway which leads to the inhibition SARS-CoV-2 infection and a reduction of the induced inflammatory response (PubMed : 35102342).
See full target information MBL2
style
left-column
style
left-column
style
right-column

Complementary Products

Assay Kits

AB193709

Human MBL ELISA Kit (Mannose-Binding Lectin)

Sandwich ELISA - Human MBL ELISA Kit (Mannose-Binding Lectin) (AB193709)

  • 0
  • 0
Primary Antibodies

AB11861

Anti-C1q antibody [7H8]

Recombinant

Immunohistochemistry (Frozen sections) - Anti-C1q antibody [7H8] (AB11861)

  • 4
  • 9
Primary Antibodies

AB200999

Anti-C3 antibody [EPR19394]

BOND RX™ Validated|RabMAb|Recombinant

Western blot - Anti-C3 antibody [EPR19394] (AB200999)

  • 4
  • 9
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB7817

Anti-alpha smooth muscle Actin antibody [1A4]

KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [1A4] (AB7817)

  • 5
  • 105

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com