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Rabbit anti-Mannose Receptor antibody ab64693 is a rabbit polyclonal antibody that is used in Mannose Receptor western blotting, IHC and immunofluorescence. Suitable for human, mouse and rat samples.

Tried and trusted by researchers since 2009
Anti-Mannose Receptor antibody ab64693 is cited in over 830 publications
Same trusted quality, New lower price!


Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA

Form

Liquid

Clonality

Polyclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PICC/IFWB
Human
Tested
Expected
Expected
Mouse
Tested
Expected
Expected
Rat
Tested
Expected
Expected
Chimpanzee
Predicted
Predicted
Predicted
Rhesus monkey
Predicted
Predicted
Predicted

Tested
Tested

Species

Mouse

Dilution info

0.05000-0.10000 µg/mL

Notes

We do not recommend methanol fixation with this antibody

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Species

Rat

Dilution info

0.05000-0.10000 µg/mL

Notes

We do not recommend methanol fixation with this antibody

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Species

Human

Dilution info

0.05000-0.10000 µg/mL

Notes

We do not recommend methanol fixation with this antibody

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Predicted
Predicted

Species

Chimpanzee, Rhesus monkey

Dilution info

-

Notes

-

Expected
Expected

Species

Human

Dilution info

1 µg/mL

Notes

Permeabilise with Tween (0.1%)

Species

Mouse

Dilution info

1 µg/mL

Notes

Permeabilise with Tween (0.1%)

Species

Rat

Dilution info

1 µg/mL

Notes

Permeabilise with Tween (0.1%)

Predicted
Predicted

Species

Chimpanzee, Rhesus monkey

Dilution info

-

Notes

-

Expected
Expected

Species

Rat

Dilution info

1 µg/mL

Notes

-

Species

Human

Dilution info

0.05-0.1 µg/mL

Notes

-

Species

Mouse

Dilution info

0.05-0.1 µg/mL

Notes

-

Predicted
Predicted

Species

Chimpanzee, Rhesus monkey

Dilution info

-

Notes

-

Target data

Function

Mediates the endocytosis of glycoproteins by macrophages. Binds both sulfated and non-sulfated polysaccharide chains.(Microbial infection) Acts as phagocytic receptor for bacteria, fungi and other pathogens.(Microbial infection) Acts as a receptor for Dengue virus envelope protein E.(Microbial infection) Interacts with Hepatitis B virus envelope protein.

Alternative names

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Rabbit anti-Mannose Receptor antibody ab64693 is a rabbit polyclonal antibody that is used in Mannose Receptor western blotting, IHC and immunofluorescence. Suitable for human, mouse and rat samples.

Tried and trusted by researchers since 2009
Anti-Mannose Receptor antibody ab64693 is cited in over 830 publications
Same trusted quality, New lower price!

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Purification technique

Affinity purification Immunogen

Specificity

From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

Biological summary

Mannose receptor performs essential functions in immune response modulation. It is not part of any known protein complex but can interact with other proteins on the cell surface. Expressed widely on macrophages it helps in the clearance of glycosylated antigens and pathogens that have been tagged for degradation. Anti-CD206 antibodies are commonly used in the study of macrophage biology helping to identify and isolate cells expressing the CD206 marker.

Mechanical summary

The Mannose Receptor also known as CD206 or MRC1 is a transmembrane protein that plays a role in the endocytic pathway. With a molecular weight of approximately 175 kDa it is expressed primarily in macrophages and dendritic cells. This receptor binds to mannose fucose and N-acetylglucosamine residues present on glycoproteins and microorganisms. It functions as an image receptor that facilitates uptake and removal of external particles.

Pathway

Mannose receptor plays a significant role in the immune system's pathogen recognition and clearance pathways. It is involved in phagocytosis and antigen processing connecting with lysosomal enzymes following endocytosis of pathogens. This receptor’s function can interface with components of the toll-like receptor (TLR) pathways where it may work alongside proteins such as CD14 in recognizing pathogen-associated molecular patterns.

Disease

Mannose receptor is implicated in infectious diseases and inflammatory conditions. It is involved in the body's response to bacterial infections aiding in the phagocytosis and clearance of these pathogens. Its role in inflammation connects it with disorders such as atherosclerosis where dysfunctional macrophage activation can be observed. The mannose receptor interacts with other proteins related to these conditions including CD209 which also participates in pathogen recognition and immune response functionalities.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Western blot - Anti-Mannose Receptor antibody (ab64693), expandable thumbnail

    Western blot - Anti-Mannose Receptor antibody (ab64693)

    All lanes: Western blot - Anti-Mannose Receptor antibody (AB64693) at 1 µg/mL

    Lane 1: Rat liver tissue lysate at 10 µg

    Lane 2: Human lung tissue lysate at 10 µg

    Lane 3: Rat lung tissue lysate at 10 µg

    Lane 4: Western blot - Mouse lung normal tissue lysate - total protein (AB29297) at 10 µg

    Secondary

    All lanes: Peroxidase AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 166 kDa

    Observed band size: 190 kDa

    Exposure time: 4min

  • Immunocytochemistry/ Immunofluorescence - Anti-Mannose Receptor antibody (ab64693), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Mannose Receptor antibody (ab64693)

    ab64693 staining Mannose Receptor in MOLT4 cells. The cells were fixed with 4% Formaldehyde (10 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab64693 at 1μg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannose Receptor antibody (ab64693), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannose Receptor antibody (ab64693)

    IHC image of ab64693 staining in human lung formalin fixed paraffin embedded tissue section*, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab64693, 0.1μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannose Receptor antibody (ab64693), expandable thumbnail
    This image is courtesy of an anonymous customer review

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannose Receptor antibody (ab64693)

    Paraffin embedded, formalin-fixed mouse lung tissue labelling the mannose receptor with ab64693 at 1/500 in immunohistochemical analysis. An Alexa Fluor® 647 Goat anti-rabbit IgG was used as the secondary antibody, this was artificially colored green by software to enhance stain visibility. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. 15% serum was used as blocking agent for 1 hour at room temperature.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannose Receptor antibody (ab64693), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannose Receptor antibody (ab64693)

    IHC image of Mannose Receptor staining in a section of formalin-fixed paraffin-embedded normal rat lung performed on a Leica BONDTM system using the standard protocol. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab64693, 0.01ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannose Receptor antibody (ab64693), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannose Receptor antibody (ab64693)

    IHC image of Mannose Receptor staining in a section of formalin-fixed paraffin-embedded normal mouse lung performed on a Leica BONDTM system using the standard protocol. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab64693, 0.01ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannose Receptor antibody (ab64693), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannose Receptor antibody (ab64693)

    Immunohistochemical analysis of formalin fixed paraffin embedded human lung labelling mannose receptor with ab64693 at 0.1 µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with an OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was conducted for 32min with ULTRA cell conditioning solution (CC1 pH8.5). ab64693 anti mannose receptor antibody was incubated at 37°C for 16min. Sections were counterstained is with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannose Receptor antibody (ab64693), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannose Receptor antibody (ab64693)

    Immunohistochemical analysis of formalin fixed paraffin embedded human lung labelling mannose receptor with ab64693 at 0.1 µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with an OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was conducted for 32min with ULTRA cell conditioning solution (CC1 pH8.5). ab64693 anti mannose receptor antibody was incubated at 37°C for 16min. Sections were counterstained is with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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