Rabbit Recombinant Monoclonal Mannose Receptor antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 9 publications.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannose Receptor antibody [EPR22489-7] (AB252921), expandable thumbnail](https://content.abcam.com/products/images/mannose-receptor-antibody-epr22489-7-ab252921--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img11544.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannose Receptor antibody [EPR22489-7] (AB252921)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannose Receptor antibody [EPR22489-7] (AB252921), expandable thumbnail](https://content.abcam.com/products/images/mannose-receptor-antibody-epr22489-7-ab252921--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img25448.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannose Receptor antibody [EPR22489-7] (AB252921)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannose Receptor antibody [EPR22489-7] (AB252921), expandable thumbnail](https://content.abcam.com/products/images/mannose-receptor-antibody-epr22489-7-ab252921--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img138098.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannose Receptor antibody [EPR22489-7] (AB252921)")
![Western blot - Anti-Mannose Receptor antibody [EPR22489-7] (AB252921), expandable thumbnail](https://content.abcam.com/products/images/mannose-receptor-antibody-epr22489-7-ab252921--western-blot-img18945.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Mannose Receptor antibody [EPR22489-7] (AB252921)")
![Western blot - Anti-Mannose Receptor antibody [EPR22489-7] (AB252921), expandable thumbnail](https://content.abcam.com/products/images/mannose-receptor-antibody-epr22489-7-ab252921--western-blot-img138179.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Mannose Receptor antibody [EPR22489-7] (AB252921)")
Publications
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- Biomolecules 13:2022The Cancer-Associated Fibroblasts-Related Gene COMP Is a Novel Predictor for Prognosis and Immunotherapy Efficacy and Is Correlated with M2 Macrophage Infiltration in Colon Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHe Ma,Qingqing Qiu,Dan Tan,Qiaofeng Chen,Yaping Liu,Bing Chen,Mingliang WangPubMed 36671447
- Cell death & disease 13:8912022NLRP6 is required for cancer-derived exosome-modified macrophage M2 polarization and promotes metastasis in small cell lung cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXinrui Rao,Xiaoshu Zhou,Geng Wang,Xiaohua Jie,Biyuan Xing,Yingzhuo Xu,Yunshang Chen,Jun Li,Kuikui Zhu,Zilong Wu,Gang Wu,Chuangyan Wu,Rui ZhouPubMed 36270983
- Frontiers in immunology 13:8693652022ILC2s expanded by exogenous IL-33 regulate CD45+CD11b+F4/80high macrophage polarization to alleviate hepatic ischemia-reperfusion injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHai-Ming Zhang,Xiao-Jie Chen,Shi-Peng Li,Jin-Ming Zhang,Jie Sun,Liu-Xin Zhou,Guang-Peng Zhou,Bin Cui,Li-Ying Sun,Zhi-Jun ZhuPubMed 35967407
- Frontiers in immunology 13:8773142022Immune Deviation in the Decidua During Term and Preterm Labor.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYing Zha,Haiyi Liu,Xingguang Lin,Long Yu,Peng Gao,Yuqi Li,Min Wu,Xun Gong,Xinyi Bian,Qi Kang,Pan Zhi,Xiaohe Dang,Jingyu Wang,Ling Feng,Fuyuan Qiao,Yafei Huang,Wanjiang ZengPubMed 35757768
- Frontiers in cell and developmental biology 9:6848422022LncRNA TCONS_00145741 Knockdown Prevents Thrombin-Induced M1 Differentiation of Microglia in Intracerebral Hemorrhage by Enhancing the Interaction Between DUSP6 and JNK.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLanxiang Wu,Qingqing Zhan,Pan Liu,Heqing Zheng,Mingxu Liu,Jun Min,Liang Xie,Wei WuPubMed 35127692
- Frontiers in oncology 11:6839512021The Epithelial to Mesenchymal Transition Related Gene Calumenin Is an Adverse Prognostic Factor of Bladder Cancer Correlated With Tumor Microenvironment Remodeling, Gene Mutation, and Ferroptosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYiHeng Du,WenHao Miao,Xiang Jiang,Jin Cao,Bo Wang,Yi Wang,Jiang Yu,XiZhi Wang,HaiTao LiuPubMed 34150647
- Cancer cell international 21:2832021The cancer-associated fibroblasts related gene CALD1 is a prognostic biomarker and correlated with immune infiltration in bladder cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYiHeng Du,Xiang Jiang,Bo Wang,Jin Cao,Yi Wang,Jiang Yu,XiZhi Wang,HaiTao LiuPubMed 34051818
- Frontiers in oncology 11:6206742021Identification and Validation of a Stromal EMT Related LncRNA Signature as a Potential Marker to Predict Bladder Cancer Outcome.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYiHeng Du,Bo Wang,Xiang Jiang,Jin Cao,Jiang Yu,Yi Wang,XiZhi Wang,HaiTao LiuPubMed 33747932
- Oncology reports 44:91-1022020Ciprofloxacin promotes polarization of CD86+CD206‑ macrophages to suppress liver cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMengtian Fan et. al.PubMed 32377744
Key facts
- Isotype
IgG1
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | WB | ICC/IF | IHC-P | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Tested | Not recommended | Tested |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/4000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Mediates the endocytosis of glycoproteins by macrophages. Binds both sulfated and non-sulfated polysaccharide chains.(Microbial infection) Acts as phagocytic receptor for bacteria, fungi and other pathogens.(Microbial infection) Acts as a receptor for Dengue virus envelope protein E.(Microbial infection) Interacts with Hepatitis B virus envelope protein.
Alternative names
Macrophage mannose receptor 1, MMR, C-type lectin domain family 13 member D, C-type lectin domain family 13 member D-like, Human mannose receptor, Macrophage mannose receptor 1-like protein 1, hMR, MRC1L1, CLEC13DL, CLEC13D, MRC1
Recommended products
Rabbit Recombinant Monoclonal Mannose Receptor antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 9 publications.
Alternative names
Macrophage mannose receptor 1, MMR, C-type lectin domain family 13 member D, C-type lectin domain family 13 member D-like, Human mannose receptor, Macrophage mannose receptor 1-like protein 1, hMR, MRC1L1, CLEC13DL, CLEC13D, MRC1
Key facts
- Isotype
IgG1
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
EPR22489-7
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The Mannose Receptor also known as CD206 or MRC1 is a type of C-type lectin receptor with a molecular weight of 175 kDa. It is expressed on the surface of macrophages and dendritic cells. This receptor binds to complex carbohydrates like mannose and fucose found on pathogen surfaces playing an important role in their recognition. Mannose Receptors act as endocytic receptors aiding in the internalization and degradation of pathogens.
- Biological function summary
The Mannose Receptor functions in pathogen recognition and clearance by binding to specific carbohydrates on the pathogen's surface. It is not part of a larger molecular complex but works independently to facilitate phagocytosis. The receptor is essential in immune responses allowing macrophages and dendritic cells to efficiently capture and process antigens for presentation to T cells. This enables the initiation of adaptive immune responses.
- Pathways
Researchers know that the Mannose Receptor plays an important role in immune signaling pathways particularly in antigen processing and presentation. It interacts with proteins involved in these pathways including those necessary for the internalization and degradation of bound antigens. Moreover it works alongside scavenger receptors contributing to the regulation of immune and inflammatory responses.
- Associated diseases and disorders
The Mannose Receptor is often linked to pulmonary tuberculosis and certain types of cancer. When macrophages express this receptor it can modulate immune responses against Mycobacterium tuberculosis by aiding in its recognition. In cancer altered expression of CD206 can indicate tumor-associated macrophages which promote tumor growth by suppressing immune responses. These connections highlight the receptor's potential as a diagnostic marker and therapeutic target.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannose Receptor antibody [EPR22489-7] (ab252921), expandable thumbnail](https://content.abcam.com/products/images/mannose-receptor-antibody-epr22489-7-ab252921--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img11544.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannose Receptor antibody [EPR22489-7] (ab252921)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannose Receptor antibody [EPR22489-7] (ab252921)
Immunohistochemical analysis of paraffin-embedded human liver tissue labeling Mannose Receptor with ab252921 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining in macrophages (Kupffer cells) of human liver (PMID: 26938527) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannose Receptor antibody [EPR22489-7] (ab252921), expandable thumbnail](https://content.abcam.com/products/images/mannose-receptor-antibody-epr22489-7-ab252921--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img25448.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannose Receptor antibody [EPR22489-7] (ab252921)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannose Receptor antibody [EPR22489-7] (ab252921)
Immunohistochemical analysis of paraffin-embedded human colon tissue labeling Mannose Receptor with ab252921 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining in macrophages of human colon (PMID: 9060831) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannose Receptor antibody [EPR22489-7] (ab252921), expandable thumbnail](https://content.abcam.com/products/images/mannose-receptor-antibody-epr22489-7-ab252921--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img138098.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannose Receptor antibody [EPR22489-7] (ab252921)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannose Receptor antibody [EPR22489-7] (ab252921)
Immunohistochemical analysis of paraffin-embedded human Karposi's sarcoma tissue labeling Mannose Receptor with ab252921 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining in tumor cells of human Kaposi's Sarcoma (PMID: 9060831) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use. Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
![Western blot - Anti-Mannose Receptor antibody [EPR22489-7] (ab252921), expandable thumbnail](https://content.abcam.com/products/images/mannose-receptor-antibody-epr22489-7-ab252921--western-blot-img18945.jpg "Western blot - Anti-Mannose Receptor antibody [EPR22489-7] (ab252921)")
Western blot - Anti-Mannose Receptor antibody [EPR22489-7] (ab252921)
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure times: Lane 1: 3 mins. Lanes 2 & 3: 26 secs. Lane 4: 10 secs.
The molecular weight observed is consistent with what has been described in the literature (PMID: 26202986; 23752129).
All lanes: Western blot - Anti-Mannose Receptor antibody [EPR22489-7] (ab252921) at 1/1000 dilution
Lane 1: Human liver lysate at 20 µg
Lane 2: Human lung lysate at 20 µg
Lane 3: Human lymph node lysate at 20 µg
Lane 4: Human placenta lysate at 20 µg
SecondaryAll lanes: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution
Predicted band size: 166 kDa
Observed band size: 175 kDa
![Western blot - Anti-Mannose Receptor antibody [EPR22489-7] (ab252921), expandable thumbnail](https://content.abcam.com/products/images/mannose-receptor-antibody-epr22489-7-ab252921--western-blot-img138179.jpg "Western blot - Anti-Mannose Receptor antibody [EPR22489-7] (ab252921)")
Western blot - Anti-Mannose Receptor antibody [EPR22489-7] (ab252921)
Blocking/Dilution buffer: 5% NFDM/TBST.
The molecular weight of 175 kDa in lane 2 observed is consistent with what has been described in the literature (PMID: 26202986; 23752129). This antibody also reacts with an unidentifiable protein around 40 kDa.
All lanes: Western blot - Anti-Mannose Receptor antibody [EPR22489-7] (ab252921) at 1/1000 dilution
Lane 1: Untreated THP-1 (human monocytic leukemia cell line) whole cell lysate at 20 µg
Lane 2: THP-1 treated with 20 ng/ml PMA for 24 hours, then treated with 20ng/ml IL-4 for 48 hours, whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 166 kDa
Observed band size: 175 kDa
Exposure time: 3min
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