Anti-CD206 antibody [EPR22489-7]

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD206 antibody [EPR22489-7] (AB252921)

Immunohistochemical analysis of paraffin-embedded human Karposi's sarcoma tissue labeling CD206 with ab252921 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining in tumor cells of human Kaposi's Sarcoma (PMID : 9060831) is observed. Counterstained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD206 antibody [EPR22489-7] (AB252921)

Immunohistochemical analysis of paraffin-embedded human liver tissue labeling CD206 with ab252921 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining in macrophages (Kupffer cells) of human liver (PMID : 26938527) is observed. Counterstained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD206 antibody [EPR22489-7] (AB252921)

Immunohistochemical analysis of paraffin-embedded human colon tissue labeling CD206 with ab252921 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining in macrophages of human colon (PMID : 9060831) is observed. Counterstained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

• WB

Unknown

Western blot - Anti-CD206 antibody [EPR22489-7] (AB252921)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure times : Lane 1 : 3 mins. Lanes 2 & 3 : 26 secs. Lane 4 : 10 secs.

The molecular weight observed is consistent with what has been described in the literature (PMID : 26202986; 23752129).

All lanes:

Western blot - Anti-CD206 antibody [EPR22489-7] (ab252921) at 1/1000 dilution

Lane 1:

Human liver lysate at 20 µg

Lane 2:

Human lung lysate at 20 µg

Lane 3:

Human lymph node lysate at 20 µg

Lane 4:

Human placenta lysate at 20 µg

Secondary

All lanes:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Predicted band size: 166 kDa

Observed band size: 175 kDa

false

• WB

Unknown

Western blot - Anti-CD206 antibody [EPR22489-7] (AB252921)

Blocking/Dilution buffer : 5% NFDM/TBST.

The molecular weight of 175 kDa in lane 2 observed is consistent with what has been described in the literature (PMID : 26202986; 23752129). This antibody also reacts with an unidentifiable protein around 40 kDa.

All lanes:

Western blot - Anti-CD206 antibody [EPR22489-7] (ab252921) at 1/1000 dilution

Lane 1:

Untreated THP-1 (human monocytic leukemia cell line) whole cell lysate at 20 µg

Lane 2:

THP-1 treated with 20 ng/ml PMA for 24 hours, then treated with 20ng/ml IL-4 for 48 hours, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 166 kDa

Observed band size: 175 kDa

false

Exposure time: 3min