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Rabbit Recombinant Monoclonal Mannose Receptor antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication.


Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannose Receptor antibody [EPR22489-7] - BSA and Azide free (AB254471), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannose Receptor antibody [EPR22489-7] - BSA and Azide free (AB254471), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannose Receptor antibody [EPR22489-7] - BSA and Azide free (AB254471), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow CytWBICC/IFIHC-P
Human
Not recommended
Not recommended
Expected
Not recommended
Tested

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

2 products for Alternative Product

3 products for Alternative Version

Target data

Function

Mediates the endocytosis of glycoproteins by macrophages. Binds both sulfated and non-sulfated polysaccharide chains.(Microbial infection) Acts as phagocytic receptor for bacteria, fungi and other pathogens.(Microbial infection) Acts as a receptor for Dengue virus envelope protein E.(Microbial infection) Interacts with Hepatitis B virus envelope protein.

Alternative names

Macrophage mannose receptor 1, MMR, C-type lectin domain family 13 member D, C-type lectin domain family 13 member D-like, Human mannose receptor, Macrophage mannose receptor 1-like protein 1, hMR, MRC1L1, CLEC13DL, CLEC13D, MRC1

Recommended products

Rabbit Recombinant Monoclonal Mannose Receptor antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR22489-7

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

ab254471 is the carrier-free version of Anti-Mannose Receptor antibody [EPR22489-7] ab252921.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The Mannose Receptor also known as CD206 or MRC1 is a type of C-type lectin receptor with a molecular weight of 175 kDa. It is expressed on the surface of macrophages and dendritic cells. This receptor binds to complex carbohydrates like mannose and fucose found on pathogen surfaces playing an important role in their recognition. Mannose Receptors act as endocytic receptors aiding in the internalization and degradation of pathogens.

Biological function summary

The Mannose Receptor functions in pathogen recognition and clearance by binding to specific carbohydrates on the pathogen's surface. It is not part of a larger molecular complex but works independently to facilitate phagocytosis. The receptor is essential in immune responses allowing macrophages and dendritic cells to efficiently capture and process antigens for presentation to T cells. This enables the initiation of adaptive immune responses.

Pathways

Researchers know that the Mannose Receptor plays an important role in immune signaling pathways particularly in antigen processing and presentation. It interacts with proteins involved in these pathways including those necessary for the internalization and degradation of bound antigens. Moreover it works alongside scavenger receptors contributing to the regulation of immune and inflammatory responses.

Associated diseases and disorders

The Mannose Receptor is often linked to pulmonary tuberculosis and certain types of cancer. When macrophages express this receptor it can modulate immune responses against Mycobacterium tuberculosis by aiding in its recognition. In cancer altered expression of CD206 can indicate tumor-associated macrophages which promote tumor growth by suppressing immune responses. These connections highlight the receptor's potential as a diagnostic marker and therapeutic target.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannose Receptor antibody [EPR22489-7] - BSA and Azide free (ab254471), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannose Receptor antibody [EPR22489-7] - BSA and Azide free (ab254471)

    Immunohistochemical analysis of paraffin-embedded human liver tissue labeling Mannose Receptor with Anti-Mannose Receptor antibody [EPR22489-7] ab252921 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining in macrophages (Kupffer cells) of human liver (PMID: 26938527) is observed. Counterstained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use. Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Mannose Receptor antibody [EPR22489-7] ab252921).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannose Receptor antibody [EPR22489-7] - BSA and Azide free (ab254471), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannose Receptor antibody [EPR22489-7] - BSA and Azide free (ab254471)

    Immunohistochemical analysis of paraffin-embedded human colon tissue labeling Mannose Receptor with Anti-Mannose Receptor antibody [EPR22489-7] ab252921 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining in macrophages of human colon (PMID: 9060831) is observed. Counterstained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use. Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Mannose Receptor antibody [EPR22489-7] ab252921).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannose Receptor antibody [EPR22489-7] - BSA and Azide free (ab254471), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannose Receptor antibody [EPR22489-7] - BSA and Azide free (ab254471)

    Immunohistochemical analysis of paraffin-embedded human Karposi's sarcoma tissue labeling Mannose Receptor with Anti-Mannose Receptor antibody [EPR22489-7] ab252921 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining in tumor cells of human Kaposi's Sarcoma (PMID: 9060831) is observed. Counterstained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use. Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Mannose Receptor antibody [EPR22489-7] ab252921).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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