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Anti-Mannose Receptor antibody [EPR25215-277] (ab300621) is a rabbit monoclonal antibody that is used to detect Mannose Receptor in Western Blot, IHC-P, IHC-Fr, ICC/IF. Suitable for Mouse, Rat samples.



- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency

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Images

Immunocytochemistry/ Immunofluorescence - Anti-Mannose Receptor antibody [EPR25215-277] (AB300621), expandable thumbnail
  • Western blot - Anti-Mannose Receptor antibody [EPR25215-277] (AB300621), expandable thumbnail
  • Western blot - Anti-Mannose Receptor antibody [EPR25215-277] (AB300621), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannose Receptor antibody [EPR25215-277] (AB300621), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannose Receptor antibody [EPR25215-277] (AB300621), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PIHC-FrICC/IFFlow CytIP
Human
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Mouse
Tested
Tested
Tested
Tested
Not recommended
Not recommended
Rat
Tested
Tested
Tested
Expected
Not recommended
Not recommended

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/2000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/2000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species
Mouse
Dilution info
1/500
Notes

-

Species
Rat
Dilution info
1/500
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/50
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Human, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

Mediates the endocytosis of glycoproteins by macrophages. Binds both sulfated and non-sulfated polysaccharide chains. Acts as phagocytic receptor for bacteria, fungi and other pathogens.

Alternative names

Recommended products

Anti-Mannose Receptor antibody [EPR25215-277] (ab300621) is a rabbit monoclonal antibody that is used to detect Mannose Receptor in Western Blot, IHC-P, IHC-Fr, ICC/IF. Suitable for Mouse, Rat samples.



- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR25215-277
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

What is this antibody validated in?


Anti-Mannose Receptor antibody [EPR25215-277] (ab300621) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), Immunocytochemistry/immunofluorescence (ICC/IF), in Mouse, Rat samples.

What is the molecular weight of Mannose Receptor?


Anti-Mannose Receptor [EPR25215-277] (ab300621) specifically detects a band for Mannose Receptor (UniProt: Q61830) at a molecular weight of 166kDa.

Recommended positive controls


WB: Mouse and rat liver and lung tissue lysates; treated Raw264.7 cell lysate.IHC-P; Mouse liver, lung cancer, spleen FFPE tissue sections; Rat liver FFPE tissue sections.IHC-Fr: Mouse and Rat spleen fresh frozen tissues.ICC/IF: Raw 264.7 cells treated with IL-4 (40 ng/ml) for 4 days, then IL-10 (40 ng/ml) for another 4 days.

Trial sizes available!


Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.Other related products


We have a range of other formats of antibody clone [EPR25215-277] also available for your convenience:
ab300621, Carrier free - Anti-Mannose Receptor antibody [EPR25215-277] - BSA and Azide free ab300622, PE - PE Anti-Mannose Receptor antibody [EPR25215-277] ab318396, APC - APC Anti-Mannose Receptor antibody [EPR25215-277] ab318499, Alexa Fluor® 488 - Alexa Fluor® 488 Anti-Mannose Receptor antibody [EPR25215-277] ab318602, Alexa Fluor® 647 - Alexa Fluor® 647 Anti-Mannose Receptor antibody [EPR25215-277] ab318705, Alexa Fluor® 594 - Alexa Fluor® 594 Anti-Mannose Receptor antibody [EPR25215-277] ab318808, Alexa Fluor® 555 - Alexa Fluor® 555 Anti-Mannose Receptor antibody [EPR25215-277] ab318908, Alexa Fluor® 750 - Alexa Fluor® 750 Anti-Mannose Receptor antibody [EPR25215-277] ab321549



Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The Mannose Receptor also known as CD206 or MRC1 is a type of C-type lectin receptor with a molecular weight of 175 kDa. It is expressed on the surface of macrophages and dendritic cells. This receptor binds to complex carbohydrates like mannose and fucose found on pathogen surfaces playing an important role in their recognition. Mannose Receptors act as endocytic receptors aiding in the internalization and degradation of pathogens.

Biological function summary

The Mannose Receptor functions in pathogen recognition and clearance by binding to specific carbohydrates on the pathogen's surface. It is not part of a larger molecular complex but works independently to facilitate phagocytosis. The receptor is essential in immune responses allowing macrophages and dendritic cells to efficiently capture and process antigens for presentation to T cells. This enables the initiation of adaptive immune responses.

Pathways

Researchers know that the Mannose Receptor plays an important role in immune signaling pathways particularly in antigen processing and presentation. It interacts with proteins involved in these pathways including those necessary for the internalization and degradation of bound antigens. Moreover it works alongside scavenger receptors contributing to the regulation of immune and inflammatory responses.

Associated diseases and disorders

The Mannose Receptor is often linked to pulmonary tuberculosis and certain types of cancer. When macrophages express this receptor it can modulate immune responses against Mycobacterium tuberculosis by aiding in its recognition. In cancer altered expression of CD206 can indicate tumor-associated macrophages which promote tumor growth by suppressing immune responses. These connections highlight the receptor's potential as a diagnostic marker and therapeutic target.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-Mannose Receptor antibody [EPR25215-277] (ab300621), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Mannose Receptor antibody [EPR25215-277] (ab300621)

    Mannose Receptor Immunocytochemistry/ Immunofluorescence staining of RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) using rabbit Anti-Mannose Receptor antibody

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) cells labelling Mannose Receptor with ab300621 at 1/50 (9.96 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/mL dilution (Green). Confocal image showing increased cytoplasmic staining in Raw 264.7 cells treated with IL-4 (40ng/ml) for 4 days, then IL-10 (40ng/ml) for another 4 days.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/mL dilution.

  • Western blot - Anti-Mannose Receptor antibody [EPR25215-277] (ab300621), expandable thumbnail

    Western blot - Anti-Mannose Receptor antibody [EPR25215-277] (ab300621)

    Mannose Receptor Western blot staining using rabbit Anti-Mannose Receptor antibody

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/1000000 dilution.

    All lanes: Western blot - Anti-Mannose Receptor antibody [EPR25215-277] (ab300621) at 1/1000 dilution

    Lane 1: Mouse liver tissue lysate at 10 µg

    Lane 2: Mouse lung tissue lysate at 10 µg

    Lane 3: Mouse spleen tissue at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 165 kDa

    Exposure time: 120s

  • Western blot - Anti-Mannose Receptor antibody [EPR25215-277] (ab300621), expandable thumbnail

    Western blot - Anti-Mannose Receptor antibody [EPR25215-277] (ab300621)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 was used as a GAPDH loading control.

    Samples are non-boiled as boiling may cause protein aggregates.
    Normal whole brain lysate contains undetectable level of Mannose Receptor in western blot testing, which was also validated by Anti-Mannose Receptor antibody [EPR6828(B)] ab125028. PMID: 11320646 reported Mannose Receptor expression in brain is at its highest in the first week of life and dramatically decreases thereafter, being maintained at a low level throughout adulthood.

    All lanes: Western blot - Anti-Mannose Receptor antibody [EPR25215-277] (ab300621) at 1/1000 dilution

    Lane 1: Mouse lung tissue lysate at 20 µg

    Lane 2: Mouse liver tissue lysate at 20 µg

    Lane 3: Mouse brain tissue lysate at 20 µg

    Lane 4: Raw264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg

    Lane 5: Raw264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) treated with 20ng/ml IL-4 and 10uM Dexamethasone for 18 hours whole cell lysate at 20 µg

    Lane 6: Rat lung tissue lysate at 20 µg

    Lane 7: Rat liver tissue lysate at 20 µg

    Lane 8: Rat brain tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 166 kDa

    Observed band size: 200 kDa

    Exposure time: 180s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannose Receptor antibody [EPR25215-277] (ab300621), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannose Receptor antibody [EPR25215-277] (ab300621)

    Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Mannose Receptor with ab300621 at 1/2000 (0.249 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on macrophages and sinusoidal endothelial cells in mouse liver (PMID: 25250030). The section was incubated with ab300621 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannose Receptor antibody [EPR25215-277] (ab300621), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannose Receptor antibody [EPR25215-277] (ab300621)

    Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling Mannose Receptor with ab300621 at 1/2000 (0.249 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on mouse spleen (PMID: 28679964). The section was incubated with ab300621 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannose Receptor antibody [EPR25215-277] (ab300621), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannose Receptor antibody [EPR25215-277] (ab300621)

    Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling Mannose Receptor with ab300621 at 1/2000 (0.249 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on macrophages and sinusoidal endothelial cells in rat liver (PMID: 25250030). The section was incubated with ab300621 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannose Receptor antibody [EPR25215-277] (ab300621), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannose Receptor antibody [EPR25215-277] (ab300621)

    Immunohistochemical analysis of paraffin-embedded Mouse lung cancer tissue labeling Mannose Receptor with ab300621 at 1/2000 (0.249 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on stroma cells of mouse lung cancer. The section was incubated with ab300621 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Frozen sections) - Anti-Mannose Receptor antibody [EPR25215-277] (ab300621), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Mannose Receptor antibody [EPR25215-277] (ab300621)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse spleen (fresh) tissue labeling Mannose Receptor with ab300621 at 1/500 (0.996 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Positive staining in the red pulp of mouse spleen is observed. The nuclear counterstain was DAPI (Blue).
    Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.
    .

  • Immunohistochemistry (Frozen sections) - Anti-Mannose Receptor antibody [EPR25215-277] (ab300621), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Mannose Receptor antibody [EPR25215-277] (ab300621)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat spleen (fresh) tissue labeling Mannose Receptor with ab300621 at 1/500 (0.996 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Positive staining in the red pulp of rat spleen is observed. The nuclear counterstain was DAPI (Blue).
    Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.
    .

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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