Anti-CD206 antibody [EPR25215-277]

• IHC-Fr

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Immunohistochemistry (Frozen sections) - Anti-CD206 antibody [EPR25215-277] (AB300621)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat spleen (fresh) tissue labeling CD206 with ab300621 at 1/500 (0.996 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Positive staining in the red pulp of rat spleen is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution. .

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-CD206 antibody [EPR25215-277] (AB300621)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse spleen (fresh) tissue labeling CD206 with ab300621 at 1/500 (0.996 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Positive staining in the red pulp of mouse spleen is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution. .

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-CD206 antibody [EPR25215-277] (AB300621)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) cells labelling CD206 with ab300621 at 1/50 (9.96 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/mL dilution (Green). Confocal image showing increased cytoplasmic staining in Raw 264.7 cells treated with IL-4 (40ng/ml) for 4 days, then IL-10 (40ng/ml) for another 4 days.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/mL dilution.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD206 antibody [EPR25215-277] (AB300621)

Immunohistochemical analysis of paraffin-embedded Mouse lung cancer tissue labeling CD206 with ab300621 at 1/2000 (0.249 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on stroma cells of mouse lung cancer. The section was incubated with ab300621 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD206 antibody [EPR25215-277] (AB300621)

Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling CD206 with ab300621 at 1/2000 (0.249 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on macrophages and sinusoidal endothelial cells in rat liver (PMID : 25250030). The section was incubated with ab300621 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD206 antibody [EPR25215-277] (AB300621)

Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling CD206 with ab300621 at 1/2000 (0.249 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on mouse spleen (PMID : 28679964). The section was incubated with ab300621 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD206 antibody [EPR25215-277] (AB300621)

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling CD206 with ab300621 at 1/2000 (0.249 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on macrophages and sinusoidal endothelial cells in mouse liver (PMID : 25250030). The section was incubated with ab300621 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• WB

Lab

Western blot - Anti-CD206 antibody [EPR25215-277] (AB300621)

Blocking and diluting buffer and concentration : 5% NFDM/TBST. ab181602 was used as a GAPDH loading control. Samples are non-boiled as boiling may cause protein aggregates. Normal whole brain lysate contains undetectable level of Mannose Receptor in western blot testing, which was also validated by ab125028. PMID : 11320646 reported Mannose Receptor expression in brain is at its highest in the first week of life and dramatically decreases thereafter, being maintained at a low level throughout adulthood.

All lanes:

Western blot - Anti-CD206 antibody [EPR25215-277] (ab300621) at 1/1000 dilution

Lane 1:

Mouse lung tissue lysate at 20 µg

Lane 2:

Mouse liver tissue lysate at 20 µg

Lane 3:

Mouse brain tissue lysate at 20 µg

Lane 4:

Raw264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg

Lane 5:

Raw264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) treated with 20ng/ml IL-4 and 10uM Dexamethasone for 18 hours whole cell lysate at 20 µg

Lane 6:

Rat lung tissue lysate at 20 µg

Lane 7:

Rat liver tissue lysate at 20 µg

Lane 8:

Rat brain tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 166 kDa

Observed band size: 200 kDa

false

Exposure time: 180s

• WB

Lab

Western blot - Anti-CD206 antibody [EPR25215-277] (AB300621)

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/1000000 dilution.

All lanes:

Western blot - Anti-CD206 antibody [EPR25215-277] (ab300621) at 1/1000 dilution

Lane 1:

Mouse liver tissue lysate at 10 µg

Lane 2:

Mouse lung tissue lysate at 10 µg

Lane 3:

Mouse spleen tissue at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 165 kDa

false

Exposure time: 120s