Anti-Mannose Receptor antibody [EPR25215-277] (ab300621) is a rabbit monoclonal antibody that is used to detect Mannose Receptor in Western Blot, IHC-P, IHC-Fr, ICC/IF. Suitable for Mouse, Rat samples.
- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
WB | IHC-P | IHC-Fr | ICC/IF | Flow Cyt | IP | |
---|---|---|---|---|---|---|
Human | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
Mouse | Tested | Tested | Tested | Tested | Not recommended | Not recommended |
Rat | Tested | Tested | Tested | Expected | Not recommended | Not recommended |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/1000 | Notes - |
Species Rat | Dilution info 1/1000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/2000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/2000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/500 | Notes - |
Species Rat | Dilution info 1/500 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/50 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Mediates the endocytosis of glycoproteins by macrophages. Binds both sulfated and non-sulfated polysaccharide chains. Acts as phagocytic receptor for bacteria, fungi and other pathogens.
CD206, Macrophage mannose receptor 1, MMR, Mrc1
Anti-Mannose Receptor antibody [EPR25215-277] (ab300621) is a rabbit monoclonal antibody that is used to detect Mannose Receptor in Western Blot, IHC-P, IHC-Fr, ICC/IF. Suitable for Mouse, Rat samples.
- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
The Mannose Receptor also known as CD206 or MRC1 is a type of C-type lectin receptor with a molecular weight of 175 kDa. It is expressed on the surface of macrophages and dendritic cells. This receptor binds to complex carbohydrates like mannose and fucose found on pathogen surfaces playing an important role in their recognition. Mannose Receptors act as endocytic receptors aiding in the internalization and degradation of pathogens.
The Mannose Receptor functions in pathogen recognition and clearance by binding to specific carbohydrates on the pathogen's surface. It is not part of a larger molecular complex but works independently to facilitate phagocytosis. The receptor is essential in immune responses allowing macrophages and dendritic cells to efficiently capture and process antigens for presentation to T cells. This enables the initiation of adaptive immune responses.
Researchers know that the Mannose Receptor plays an important role in immune signaling pathways particularly in antigen processing and presentation. It interacts with proteins involved in these pathways including those necessary for the internalization and degradation of bound antigens. Moreover it works alongside scavenger receptors contributing to the regulation of immune and inflammatory responses.
The Mannose Receptor is often linked to pulmonary tuberculosis and certain types of cancer. When macrophages express this receptor it can modulate immune responses against Mycobacterium tuberculosis by aiding in its recognition. In cancer altered expression of CD206 can indicate tumor-associated macrophages which promote tumor growth by suppressing immune responses. These connections highlight the receptor's potential as a diagnostic marker and therapeutic target.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Mannose Receptor Immunocytochemistry/ Immunofluorescence staining of RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) using rabbit Anti-Mannose Receptor antibody
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) cells labelling Mannose Receptor with ab300621 at 1/50 (9.96 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/mL dilution (Green). Confocal image showing increased cytoplasmic staining in Raw 264.7 cells treated with IL-4 (40ng/ml) for 4 days, then IL-10 (40ng/ml) for another 4 days.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/mL dilution.
Mannose Receptor Western blot staining using rabbit Anti-Mannose Receptor antibody
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/1000000 dilution.
All lanes: Western blot - Anti-Mannose Receptor antibody [EPR25215-277] (ab300621) at 1/1000 dilution
Lane 1: Mouse liver tissue lysate at 10 µg
Lane 2: Mouse lung tissue lysate at 10 µg
Lane 3: Mouse spleen tissue at 10 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 165 kDa
Exposure time: 120s
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 was used as a GAPDH loading control.
Samples are non-boiled as boiling may cause protein aggregates.
Normal whole brain lysate contains undetectable level of Mannose Receptor in western blot testing, which was also validated by Anti-Mannose Receptor antibody [EPR6828(B)] ab125028. PMID: 11320646 reported Mannose Receptor expression in brain is at its highest in the first week of life and dramatically decreases thereafter, being maintained at a low level throughout adulthood.
All lanes: Western blot - Anti-Mannose Receptor antibody [EPR25215-277] (ab300621) at 1/1000 dilution
Lane 1: Mouse lung tissue lysate at 20 µg
Lane 2: Mouse liver tissue lysate at 20 µg
Lane 3: Mouse brain tissue lysate at 20 µg
Lane 4: Raw264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg
Lane 5: Raw264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) treated with 20ng/ml IL-4 and 10uM Dexamethasone for 18 hours whole cell lysate at 20 µg
Lane 6: Rat lung tissue lysate at 20 µg
Lane 7: Rat liver tissue lysate at 20 µg
Lane 8: Rat brain tissue lysate at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 166 kDa
Observed band size: 200 kDa
Exposure time: 180s
Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Mannose Receptor with ab300621 at 1/2000 (0.249 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Positive staining on macrophages and sinusoidal endothelial cells in mouse liver (PMID: 25250030). The section was incubated with ab300621 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling Mannose Receptor with ab300621 at 1/2000 (0.249 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Positive staining on mouse spleen (PMID: 28679964). The section was incubated with ab300621 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling Mannose Receptor with ab300621 at 1/2000 (0.249 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Positive staining on macrophages and sinusoidal endothelial cells in rat liver (PMID: 25250030). The section was incubated with ab300621 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemical analysis of paraffin-embedded Mouse lung cancer tissue labeling Mannose Receptor with ab300621 at 1/2000 (0.249 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Positive staining on stroma cells of mouse lung cancer. The section was incubated with ab300621 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse spleen (fresh) tissue labeling Mannose Receptor with ab300621 at 1/500 (0.996 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Positive staining in the red pulp of mouse spleen is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.
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Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat spleen (fresh) tissue labeling Mannose Receptor with ab300621 at 1/500 (0.996 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Positive staining in the red pulp of rat spleen is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.
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