Anti-CD206 antibody [EPR6828(B)]

• WB

Lab

Western blot - Anti-CD206 antibody [EPR6828(B)] (AB125028)

Blocking/Diluting buffer and concentration : 5% NFDM/TBST.

The expression profile observed in U-937 and THP-1 is consistent with the literature (PMID : 20801507 and PMID : 30591689). Rabbit monoclonal [EPR16891] to GAPDH (ab181602) used as loading control.

All lanes:

Western blot - Anti-CD206 antibody [EPR6828(B)] (ab125028) at 1/1000 dilution

Lane 1:

Human lung tissue lysate at 20 µg

Lane 2:

Human brain tissue lysate at 20 µg

Lane 3:

Mouse brain tissue lysate at 20 µg

Lane 4:

U-937 (Human histiocytic lymphoma monocyte) whole cell lysate at 20 µg

Lane 5:

THP-1 (Human monocytic leukemia monocyte) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 166 kDa

Observed band size: 160 kDa,200 kDa,65 kDa

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Exposure time: 20s

• OI-RD Scanning

Unknown

OI-RD Scanning - Anti-CD206 antibody [EPR6828(B)] (AB125028)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

• WB

CiteAb

Western blot - Anti-CD206 antibody [EPR6828(B)] (AB125028)

Mannose Receptor western blot using anti-Mannose Receptor antibody [EPR6828(B)] ab125028. Publication image and figure legend from Pan, C., Fujiwara, Y., et al., 2020, Front Pharmacol, PubMed 32256354.

ab125028 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab125028 please see the product overview.

Effects of compounds isolated from Epimedii Herba on macrophage activation. (A) HMDMs were incubated with compounds (5 μM) in the presence of IL-10 (20 ng/mL) for 24 h, followed by measurement of CD163 expression using cell-ELISA. (B) HMDMs were incubated with the indicated concentrations of the four flavonoid compounds for 24 h, followed by the determination of cell viability using WST-8 assay. (C) HMDMs were incubated with the four flavonoid compounds (5 μM) for 24 h in the presence of tumor culture supernatant (TCS), followed by the determination of IL-10, TNF-α, and IL-1β secretion by ELISA. (D) HMDMs were incubated with flavonoid compounds (5 μM) in the presence of IL-4 (20 nM) for 24 h, followed by the determination of CD206 and β-actin protein expression by western blot analysis. (E) HMDMs were incubated with the four flavonoid compounds (5 μM) in the presence of IL-10 (20 nM) for 6 h, followed by the determination of phosphorylated JAK1, phosphorylated STAT3, STAT3, and β-actin protein expression by western blot analysis. Experiments ware repeated three times with almost identical results. The data are presented as mean ± SD. *p < 0.05, **p < 0.01 vs. control.

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