AB224611

Anti-Mannosidase II/MAN2A1 antibody

Name: Anti-Mannosidase II/MAN2A1 antibody (ab224611) | Abcam
Brand: Abcam Limited
SKU: AB224611
Price: 525 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal Mannosidase II/MAN2A1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MAN2A1 aa 1-200.

View Alternative Names

MANA2, MAN2A1, Alpha-mannosidase 2, Golgi alpha-mannosidase II, Mannosidase alpha class 2A member 1, AMan II, Man II

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-Mannosidase II/MAN2A1 antibody (AB224611)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Mannosidase II/MAN2A1 antibody (AB224611)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Mannosidase II/MAN2A1 (green) using ab224611 (1/100 dilution) in ICC/IF. Secondary : Alexa Fluor 488^® conjugated Goat Anti-Rabbit IgG (H+L).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannosidase II/MAN2A1 antibody (AB224611)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannosidase II/MAN2A1 antibody (AB224611)

Paraffin embedded human lung cancer tissue stained for Mannosidase II/MAN2A1 with ab224611 (1/100 dilution) in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannosidase II/MAN2A1 antibody (AB224611)

Paraffin embedded human cervical cancer tissue stained for Mannosidase II/MAN2A1 with ab224611 (1/100 dilution) in immunohistochemical analysis.

Supplier Data

Western blot - Anti-Mannosidase II/MAN2A1 antibody (AB224611)

All lanes:

Western blot - Anti-Mannosidase II/MAN2A1 antibody (ab224611) at 1/500 dilution

Lane 1:

HEK-293 (human epithelial cell line from embryonic kidney) cell lysate

Lane 2:

HepG2 (human liver hepatocellular carcinoma cell line) cell lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 131 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human MAN2A1 aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

Q16706

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Mannosidase II also known as MAN2A1 is an enzyme that plays a mechanical role in the processing of N-linked oligosaccharides. It performs this task by cleaving mannose residues from glycoproteins during the process of glycan maturation. MAN2A1 has a mass of about 133 kDa and is predominantly located in the Golgi apparatus an important cellular organelle involved in modifying sorting and packaging proteins. This enzyme shows expression in various types of tissues contributing to different levels of glycoprotein processing in the human body.

Biological function summary

Mannosidase II is involved in glycoprotein maturation and functions as an important component within the N-glycan biosynthesis pathway. It does not form a permanent complex with other enzymes but interacts with other glycosylation enzymes to ensure sequential processing. This enzyme helps in maintaining the consistency and specificity of glycoprotein folding and function impacting cellular communication and stability. Various cells across tissues utilize glycoproteins for different cellular processes illustrating MAN2A1's importance in molecular biology.

Pathways

MAN2A1 plays a significant role in the N-glycan biosynthesis and quality control pathway. This pathway involves a cascade of enzymatic actions facilitating proper protein folding and cellular signaling. Within these pathways other essential proteins such as alpha-mannosidase I and N-acetylglucosaminyltransferase I work in tandem with mannosidase II. MAN2A1 contributes to the precision of glycosylation alterations affecting signal transductions and cell-cell interactions important in various physiological processes.

MAN2A1 associates with congenital disorders of glycosylation and certain types of cancer including hepatocellular carcinoma. Improper function or expression of this enzyme can lead to aberrant glycosylation patterns contributing to these diseases. In cancer progression glycosylation changes influence cell adhesion and immune surveillance affecting tumor behavior and metastasis. MAN2A1 can indirectly interact with proteins like E-cadherin through altered glycosylation influencing cell-cell adhesion and cancer development.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Catalyzes the first committed step in the biosynthesis of complex N-glycans. It controls conversion of high mannose to complex N-glycans; the final hydrolytic step in the N-glycan maturation pathway.

See full target information MAN2A1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Bioengineered 12:10073-10088 PubMed34709988

2021

Screening and cellular validation of prognostic genes regulated by super enhancers in oral squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Liru Zhang,Huanju Li,Yongle Qiu,Yuanhang Liu,Xin Liu,Wenjing Wang

Folia histochemica et cytobiologica 59:134-143 PubMed34151999

2021

Golgi a-mannosidase II mediates the formation of vascular smooth muscle foam cells under inflammatory stress.

Applications

Unspecified application

Species

Unspecified reactive species

Kelan Zha,Qiang Ye

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Mannosidase II/MAN2A1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-Mannosidase II/MAN2A1 antibody (AB224611)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannosidase II/MAN2A1 antibody (AB224611)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannosidase II/MAN2A1 antibody (AB224611)

Western blot - Anti-Mannosidase II/MAN2A1 antibody (AB224611)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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